CTGF: Human Connective Tissue Growth Factor
Description
BACKGROUND Connective Tissue Growth Factor (CTGF) is a member of cysteine rich regulatory proteins
Details
Cat. No.:
RP1060
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Alternative Name:
CCN2, HCS24
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Source:
E. coli
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Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
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Formulation:
Recombinant human CTGF is lyophilized from 1.0 mg/ml 10 mM NaAcetate, pH 6.0.
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Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
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Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product at a concentration of 0.1 mg/ml with 5 mM NaAcetate, pH 6.0.
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Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
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Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
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Biological Activity:
The activity is determined by the dose-dependent stimulation of HUVEC