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Cytofect™ Epithelial Cell Transfection Kit

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Description

CAI Box Total Kit

Transfection, the introduction of nucleic acid into mammalian cells, is a powerful technique used to study in vivo gene function and regulation. The Cytofect™ Epithelial Cell Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into a wide variety of epithelial cells. This kit contains Cytofect-1, Enhancer and Media for transfection and culturing cells. Viral Enhancer has the ability to complex with unmodified DNA via cationic Cytofect-1 and protect the transfected DNA from lysosomal degradation.  This results in high transfection efficiency and viability for these traditionally hard-to-transfect primary epithelial cells.

#Wells / Rxns

96 / 620

24 / 124

12 / 62

The Kit contains all necessary reagents, media and optimized protocols to carry out successful transfection of epithelial cells with high efficiency (40%-70%) and viability (70%-95%).

Products are for research use only. They are not intended for human, animal, or diagnostic applications. *Viral Enhancer (VE) is a replication-deficient, Adenovirus-derived formulation that does not contain replication competent virus. TR1 and VE are manufactured by Targeting Systems.

Details

Kit Contents:
Cyrofect-1
Viral Enhancer (VE)*
Antibiotics-Free Growth Medium
Transfection Medium
Transfection Medium with Serum
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
Instructions TF102K

Format: PDF

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Instructions TF102KS

Format: PDF

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