Transfection of Rat Hepatocytes with a GFP-expression plasmid using the Cytofect™ Hepatocyte Transfection Kit.
Cytofect™ Hepatocyte Transfection Kit
Description
Transfection, the introduction of nucleic acid into mammalian cells, is a powerful technique used to study in vivo gene function and regulation. The Cytofect™ Hepatocyte Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into hepatocytes. This kit contains Cytofect-1, Viral Enhancer* and Media for transfection and culturing cells. Viral Enhancer complexes with DNA and Cytofect-1 and escorts the transfection complex to the nucleus. This results in high transfection efficiency and viability for these traditionally hard-to-transfect primary cells.
#Wells / Rxns
96 / 1000
24 / 200
12 / 100
The Kit contains all necessary reagents, media and optimized protocols to carry out successful transfection of primary Hepatocytes with high efficiency (40%) and viability (70%-95%).
Products are for research use only. They are not intended for human, animal, or diagnostic applications. Viral Enhancer (VE) is a replication-deficient, Adenovirus-derived formulation that does not contain replication competent virus. TR1 and VE are manufactured by Targeting Systems.
Details
| Kit Contents: | Cytofect -1 Viral Enhancer (VE) Antibiotics-Free Growth Medium Transfection Medium |
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Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
FAQs
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Cytofect™ Transfection Reagents: Frequently Asked Questions (FAQs)
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