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Cytofect™ Chondrocyte Transfection Kit

Transfection of adult HC (Cat. No. 402-05a) with a GFP-expression plasmid using CF2 with VE*.

Cytofect™ Chondrocyte Transfection Kit

The Cytofect™ Chondrocyte Transfection Kit is a plasmid DNA delivery system optimized for transfection of primary human chondrocytes, including articular chondrocytes isolated from diarthrodial joints.

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Description

The Cytofect™ Chondrocyte Transfection Kit is a plasmid DNA delivery system optimized for transfection of primary human chondrocytes, including articular chondrocytes isolated from diarthrodial joints. Primary chondrocytes can be difficult to transfect while maintaining viability and characteristics associated with their native phenotype. The kit contains Cytofect-2, Viral Enhancer, transfection medium, and antibiotic-free growth medium. The Viral Enhancer is an endosomolytic peptide formulation that complexes with DNA and Cytofect-2 and promotes delivery of the transfection complex toward the nucleus. This formulation is designed to support plasmid delivery, cell viability, and transgene expression in primary chondrocytes, with approximately 35% transfection efficiency and 80–95% viability for human chondrocytes under specified conditions; actual results may vary with cell source, passage, plasmid, and experimental conditions.

Chondrocytes are the principal resident cells of cartilage. They are found in specialized tissues including articular cartilage covering the surfaces of synovial joints, the intervertebral discs, the nasal septum, and the tracheal rings. Articular cartilage is an avascular, generally aneural tissue in which chondrocytes are embedded within a dense extracellular matrix. Although the articular surface contacts synovial fluid, chondrocytes are surrounded by cartilage matrix and receive nutrients primarily through diffusion. During development, chondrocytes arise through the chondrogenic differentiation of mesenchymal progenitor cells. In culture, primary chondrocytes may proliferate and can undergo phenotypic changes, including loss of their rounded morphology and reduced expression of cartilage-associated genes with extended expansion.

The primary function of chondrocytes is to synthesize, organize, and maintain the cartilage extracellular matrix. In articular cartilage, this matrix contains abundant type II collagen and aggrecan. The collagen network provides tensile reinforcement and structural organization, while aggrecan-associated proteoglycans help retain water and contribute to resistance against compressive loading. Chondrocytes also regulate matrix turnover by producing matrix components and remodeling enzymes and by responding to mechanical, inflammatory, and biochemical signals.

By enabling plasmid DNA delivery into cultured primary chondrocytes, the Cytofect™ Chondrocyte Transfection Kit supports research into cartilage development, extracellular matrix synthesis, inflammatory signaling, mechanotransduction, and chondrocyte phenotype. Transfected chondrocyte models can be used to investigate gene function and gene regulation, examine pathways involved in cartilage degradation and repair, and evaluate candidate therapeutics for osteoarthritis and other joint diseases. These models can also support regenerative medicine research and the development of experimental approaches that are subsequently evaluated in three-dimensional cultures, explants, animal models, or other translational systems. The kit is intended for research use only and is not intended for human, animal, or diagnostic applications.

Transfection, the introduction of DNA into cells, is a powerful technique used to study in vivo gene function and regulation. Cytofect™ Chondrocyte Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into chondrocytes. This kit contains Cytofect-2, Viral Enhancer* and Media for transfection and culturing cells. Viral Enhancer complexes with DNA and Cytofect-2 and escorts the tranfection complex to the nucleus. This results in high transfection efficiency and viability for these traditionally hard-to-transfect primary cells.

#Wells / Rxns

96 / 875

24 / 175

12 / 86

The Kit contains all necessary reagents, media and optimized protocols to carry out successful transfection of Human Chondrocytes with good efficiency (35%) and viability (80%-95%).

Products are for research use only. They are not intended for human, animal, or diagnostic applications. Viral Enhancer (VE) is a replication-deficient, Adenovirus-derived formulation that does not contain replication competent virus. TR1 and VE are manufactured by Targeting Systems.

Details

Kit Contents: Cytofect -2
Viral Enhancer (VE)
Antibiotics-Free Growth Medium
Transfection Medium
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
Instructions TF402K

Format: PDF

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Instructions TF402KS

Format: PDF

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FAQs

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Cytofect™ Transfection Reagents: Frequently Asked Questions (FAQs)
Need More Help?

Visit our comprehensive FAQ page for detailed answers to common questions.

Cytofect™ Transfection Reagents: Frequently Asked Questions (FAQs)

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