Human Chondrocyte Media for use with cartilage-producing cells.
Human Chondrocyte Media
HC Growth Medium: All-in-one ready-to-use
Type: Growth Medium
Size: 500 ml
Price: $140.00
Description
Specialized media, with ingredients and supplements optimized for the unique requirements of Human Chondrocytes (HC), providing optimal culture conditions, cell health, data integrity and reproducibility.
Use with:
- Human Chondrocytes – Osteoarthritis: HC-OA
- Human Chondrocytes – Re-Differentiated: RD-HC
- Human Chondrocytes – Rheumatoid Arthritis: HC-RA
- Human Chondrocytes: HC
CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions. Â The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Details
Chondrogenesis Kit (Cat. No. 072K)
| Kit Component | Cat. No. | Storage | |||
|---|---|---|---|---|---|
| Sodium Alginate Solution | 072-25 | 25C | |||
| Sodium Chloride Solution | 074-100 | 25C | |||
| Calcium Chloride Solution | 071-100 | 25C | |||
| Depolymerization Solution | 073-50 | 25C | |||
| Chondrocyte Differentiation Medium | 411D-250 | 4C |
Sodium Alginate Solution
Cat. No. 072-25 / 25 ml
Sterile 1.2% solution, ready-to-use
Sodium Chloride Solution: NaCl
Cat. No. 074-100 / 100 ml
Sterile filtered 0.155 M saline solution, ready-to-use
Calcium Chloride Solution: CaCl2
Cat. No. 071-100 / 100 ml
Sterile, ready-to-use
Depolymerization Solution: DS
Cat. No. 073-50 / 50 ml
Sterile-filtered sodium citrate solution, ready-to-use
Chondrocyte Differentiation Medium
Cat. No. 411D-250
Ready-to-use
Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.
- Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.
- Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads
- Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.
- Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads
- Remove Chondrocyte Differentiation Medium
- Wash the beads 2 times with 0.155 M NaCl
- Add 3 volume of Depolymerizatiojn Solution per volume of packed alginate beads
- Mix at room temperature for 30 minutes
- Apply gentle agitation until the beads have completely dissolved
- Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
- Wash the cells 2 times with 0.155 M NaCl