i-HCm Differentiation Medium Kit is used to derive Cardiomyocytes (i-HCm) from Human Induced Pluripotent Stem Cells.
Human Cardiomyocyte Differentiation Media
Human Cardiomyocyte Differentiation Medium Kit: Differentiation, Selection & Maintenance Media sold together, packaged separately. See Details Tab.
Size: 1 Kit
Price: $303.00
Description
News Release
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From Skin Cells to Beating Heart Cells Dermal fibroblasts (HDF) isolated from human skin, reprogrammed to pluripotent stem cells (HiPSC), then differentiated to cardiomyocytes (i-HCm). (a) In vitro-cultured i-HCm immunofluorescently stained for Alpha Actinin (b) Troponin, and nuclei counter-stained for DAPI. (c) Pulsating heart tissue spheroid (organoid) constructed with Cyfuse Regenova Bio 3D Printer, incorporating (left) neonatal or (right) adult i-HCm & primary cardiac cells. Printed i-HCm derived from adult iPSC exhibit strong beating at 72 pulses per minute while neonatal i-HCm demonstrate weaker, faster beat rate. (d): Monolayer-cultured i-HCm beat spontaneously in rhythm.
Human Cardiomyocyte Differentiation Media are specialized formulations designed to guide human pluripotent stem cells into functional cardiomyocytes under controlled culture conditions. In vivo, cardiomyocytes are the contractile muscle cells of the heart, responsible for generating the mechanical force that drives blood circulation. In vitro, these cells model key aspects of human cardiac development and pathophysiology, enabling researchers to investigate how cardiomyocyte differentiation affects expression, electrophysiological behavior, structural maturation, and responses to biological or chemical stimuli.
These media are applied in stepwise differentiation protocols starting from human pluripotent stem cells (HPSC), including human induced pluripotent stem cell (HiPSC) lines. As differentiation proceeds, developing cell populations acquire cardiac lineage-specific markers and progressively mature, yielding HiPSC-derived cardiomyocytes as a standardized, human-relevant cell source. Researchers routinely characterize differentiation efficiency using flow cytometry and functional readouts to assess lineage purity and maintain reproducibility across differentiation runs and iPSC/HiPSC line backgrounds.
Human Cardiomyocyte Differentiation Media support research and translational applications by providing a consistent environment for scalable cardiomyocyte production. In safety pharmacology and drug screening, HiPSC-derived cardiomyocytes generated with these media provide an essential human model for screening compound efficacy, electrophysiological effects, and cardiotoxicity phenotypes. In disease modeling, cardiac differentiation from patient-derived HPSC lines allows investigators to link genetic variation to alterations in differentiation efficiency, sarcomeric organization, and functional performance.
Human Cardiomyocyte Differentiation Media enable the reliable generation of functional cardiomyocytes from pluripotent stem cells — bridging developmental biology with assay-driven protocol workflows and supporting reproducible investigations into human cardiac function, expression changes, disease mechanisms, and therapeutic response.
Medium carefully optimized for differentiating Human Induced Pluripotent Stem Cells into iPSC-Derived Cardiomyocytes and maintaining i-HCm. Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.
Use with: Human Induced Pluripotent Stem Cells
Details
HiPSC to i-HCm timeline using Differentiation Medium Kit (017DK) 
i-HCm Differentiation Medium Kit, Xeno-Free (017DK)
| Cat. No. | Medium | Abbrev | Vol |
| 017D1-30 | Differentiation 1 | i-HCm DM1 | 30ml |
| 017D2-30 | Differentiation 2 | i-HCm DM2 | 30ml |
| 017D3-100 | Differentiation 3 | i-HCm DM3 | 100ml |
| 019-50 | Selection | i-HCm SM | 50ml |
| 021-30 | Maintenance | i-HCm MM | 30ml |
Store all kit components at 4°C. Stable for 4 weeks at 4ºC.