Human Smooth Muscle Cell Differentiation Medium
Human Smooth Muscle Cell Differentiation Medium
Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized
Size: 250 ml
Type: Differentiation Medium
Price: $108.00
Description
Human Smooth Muscle Cell Differentiation Medium is formulated to support phenotypic maturation of primary human smooth muscle cells in culture. During in vitro expansion, vascular smooth muscle cells can shift from a contractile, relatively quiescent phenotype toward a more synthetic, proliferative, and migratory state. Differentiation conditions can help support re-expression of contractile characteristics and provide a more physiologically relevant culture model for vascular research.
In the human cardiovascular system, vascular smooth muscle cells are located primarily in the tunica media of every major blood vessel — including the aorta, coronary artery branches, systemic artery networks, and vein structures — with the amount and organization of smooth muscle varying by vessel size and type. Small capillary beds generally lack a conventional smooth muscle layer and are instead associated with pericytes. Vascular smooth muscle cells regulate vessel diameter and vascular tone through involuntary contraction and relaxation in response to autonomic, endothelial, hormonal, paracrine, and mechanical signals. These responses work in tandem with cardiac output to regulate vascular resistance, blood flow, and tissue perfusion.
Human Smooth Muscle Cell Differentiation Medium is intended to provide culture conditions that support a contractile smooth muscle phenotype and the expression of associated markers, including smooth muscle α-actin (α-SMA), calponin, SM22α, and smooth muscle myosin heavy chain (MYH11). Marker expression should be evaluated together with appropriate functional assays when contractile performance is an experimental endpoint.
In translational and cardiovascular research, differentiated human smooth muscle cell cultures may be used to:
- Model vascular phenotypic switching: Investigate changes between contractile, synthetic, proliferative, and migratory states relevant to atherosclerosis, restenosis, vascular calcification, and arterial remodeling. Vascular smooth muscle cells can contribute to atherosclerotic plaque development and stability through migration, extracellular-matrix production, proliferation, and adoption of disease-associated phenotypes.
- Evaluate contractile responses: Assess agonist-induced contraction or force-related responses using methods such as traction-force microscopy, collagen-gel contraction assays, live-cell imaging, or microfluidic vessel-on-a-chip systems, where appropriate.
- Screen cardiovascular therapeutics: Evaluate compounds that affect vascular smooth muscle contraction, proliferation, migration, remodeling, hypertension-related pathways, or vascular disease mechanisms.
- Support tissue-engineering studies: Use primary human smooth muscle cells in two-dimensional or three-dimensional culture systems to investigate phenotypic maturation, extracellular-matrix organization, and the development of engineered vascular constructs.
Medium carefully optimized for the characteristics and requirements unique to the differentiation of smooth muscle cells. Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.
Use with:
- Human Aortic Smooth Muscle Cells: HAOSMC
- Human Brachiocephalic Artery Smooth Muscle Cells: HBcASMC
- Human Carotid Artery Smooth Muscle Cells: HCtASMC
- Human Coronary Artery Smooth Muscle Cells: HCASMC
- Human Internal Thoracic Artery Smooth Muscle Cells: HITASMC
- Human Pulmonary Artery Smooth Muscle Cells: HPASMC
- Human Subclavian Artery Smooth Muscle Cells: HScASMC
- Human Umbilical Artery Smooth Muscle Cells: HUASMC
- Human Umbilical Vein Smooth Muscle Cells: HUVSMC
Details
Citations
Powered by BiozExtended Family Products
Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately
Human SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use.
Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium