Neuron Freezing Medium
Freezing Medium for Neurons wo Serum: Serum-free freezing medium specialized for neurons
Size: 50 ml
Price: $103.00
Description
Neuron Freezing Medium is a specialized cryopreservation formulation designed to help preserve sensitive cells, including neuron populations, during freezing and thawing. It is intended to support post-thaw viability and recovery for downstream research applications.
A neuron is a highly polarized cell with a central soma, branching dendrites, and a long axon. During development and in culture, neurons extend neurites that mature into axons and dendrites and support synapse formation within the brain, including the cerebral cortex and striatum.
Neuronal populations are found throughout the central and peripheral nervous systems, including cortical regions of the cerebral cortex, the spinal cord, and peripheral ganglia such as dorsal root ganglion neurons. These sensory neurons detect environmental stimuli and transmit signals through peripheral nerves to the central nervous system. In the striatum, medium spiny neurons are influenced by dopamine signaling and receptor pathways. Beyond primary tissue, continuous models such as neuroblastoma lines and hybridoma cell models are also used in neurological research.
For studies of neurogenesis, neurotransmission, and neurodegenerative disease, reliable access to healthy cultures is essential. Neuron Freezing Medium helps provide a protective environment for storing neuron cells and related models with consistency and reproducibility.
Freezing Media (also referred to as cryopreservation media) is a specialized category of cell culture formulation engineered to protect cells from ice crystal formation and osmotic shock during the freezing and thawing processes.
The primary biological and physical function of freezing media is to manage the thermodynamic and osmotic transitions that occur during cooling. While CPAs do lower the freezing point of water modestly, their protective action is primarily colligative; they modify the concentration of solutes inside and outside the cell and fundamentally alter ice crystallization kinetics. As the extracellular environment freezes, ice crystals exclude solutes, creating a hyperosmotic extracellular fraction that draws water out of the cells. If cooling occurs too rapidly, intracellular water cannot escape fast enough, leading to lethal intracellular ice crystallization that shears the plasma membrane and organelle boundaries. Conversely, if cooling is too slow, prolonged exposure to severe hyperosmotic stress leads to excessive cellular dehydration and solute toxicity.
Freezing media minimize these dual mechanisms of injury. Permeating CPAs enter the cytoplasm to displace water and disrupt the ordered lattice structure of ice, while non-permeating components increase extracellular viscosity and stabilize lipids within the plasma membrane bilayer. This coordinated protection preserves critical baseline cell biology features, maintaining intact cell surface receptor baselines and functional enzymatic pathways.
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