Porcine Endothelial Cell Media, optimized with components and supplements tailored to swine aortic and arterial cells.
Porcine Endothelial Cell Media
Porcine EC Growth Medium: All-in-one ready-to-use
Type: Growth Medium
Size: 500 ml
Price: $142.00
Description
Porcine Endothelial Cell Media are endothelial cell media optimized for the growth and maintenance of porcine vascular endothelial cells in vitro. The formulation is intended for porcine primary cells, including porcine aortic endothelial cells, porcine coronary artery endothelial cells, and porcine pulmonary artery endothelial cells. These cells are isolated from the inner lining of large blood vessels and provide relevant models for vascular biology, cardiovascular research, inflammation, drug transport, and translational studies using the pig as a large-animal model.
An endothelial cell is a specialized vascular cell that forms the endothelium, a thin, continuous layer lining arteries, veins, capillaries, and specialized vascular beds. Endothelial cells regulate exchange between blood and tissues, maintain vascular-barrier integrity, modulate vascular tone and blood flow, control leukocyte adhesion and transmigration, and participate in coagulation, inflammation, and angiogenesis. Their phenotype varies according to the vessel and organ of origin. For example, coronary and aortic endothelial cells model large-vessel biology, whereas microvascular or brain endothelial cells have specialized barrier and transport properties.
Porcine endothelial cells are valuable for cell biology and molecular biology because the porcine cardiovascular system shares important anatomical and physiological features with humans. Cultures of porcine endothelial cells can be used to study endothelial activation, barrier function, vascular permeability, angiogenesis, cell adhesion, leukocyte transmigration, oxidative or inflammatory injury, and responses to pharmacological compounds. In one cardiovascular study, porcine coronary artery endothelial cells maintained in Porcine Endothelial Cell Growth Medium were used in adhesion and neutrophil-transmigration assays.
The basal medium and endothelial cell growth supplement provide nutrients and cell-supportive signals that help maintain endothelial-cell viability, attachment, morphology, and proliferative capacity. Formulation-specific supplements may include serum, growth factors, hormones, amino acids, vitamins, and other nutrients required by primary endothelial cells. The product is available as ready-to-use growth medium, a basal medium with separately supplied growth supplement, and versions formulated without antibiotics or serum, allowing researchers to adapt the culture system to the requirements of their assay.
By supporting healthy and reproducible cell culture media conditions, Porcine Endothelial Cell Media helps researchers establish endothelial monolayers and generate consistent cultures for permeability assays, angiogenesis or tube-formation assays, inflammation studies, vascular-device testing, gene-transfer experiments, and endothelial-specific adenoviral overexpression. Porcine brain microvascular endothelial cells may also be used in specialized blood–brain barrier models, but brain-derived endothelial cells generally require organ-specific validation and may not respond identically to media optimized for aortic, coronary, or pulmonary artery cells.
The media are intended primarily for porcine endothelial cells and should not be assumed to support all other species or cell types. Mouse cells, rat cells, rabbit cells, canine cells, monkey cells, hamster cells, human primary cells, and other primary cells may require species- or tissue-specific media. Similarly, epithelial cells, immune cells, primary hepatocytes, liver cells, tumor endothelial cells, and immortalized cells can have distinct nutritional and signaling requirements. These models may be available through specialized cell biologics, cell culture, or custom cell-isolation resources, but their compatibility with this product should be experimentally validated rather than inferred.
Medium carefully optimized for the characteristics and requirements unique to Porcine Endothelial Cells. Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.
Details
CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions. The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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