Instructions 017DK i-HCm Differentiation Medium Kit
MSDS 017DK
Description
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From Skin Cells to Beating Heart Cells Dermal fibroblasts (HDF) isolated from human skin, reprogrammed to pluripotent stem cells (HiPSC), then differentiated to cardiomyocytes (i-HCm). (a) In vitro-cultured i-HCm immunofluorescently stained for Alpha Actinin (b) Troponin, and nuclei counter-stained for DAPI. (c) Pulsating heart tissue spheroid (organoid) constructed with Cyfuse Regenova Bio 3D Printer, incorporating (left) neonatal or (right) adult i-HCm & primary cardiac cells. Printed i-HCm derived from adult iPSC exhibit strong beating at 72 pulses per minute while neonatal i-HCm demonstrate weaker, faster beat rate. (d): Monolayer-cultured i-HCm beat spontaneously in rhythm.
Medium carefully optimized for differentiating Human Induced Pluripotent Stem Cells into iPSC-Derived Cardiomyocytes and maintaining i-HCm. Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.
Use with: Human Induced Pluripotent Stem Cells
(Click to Enlarge) i-HCm Differentiation Medium Kit is used to derive Cardiomyocytes (i-HCm) from Human Induced Pluripotent Stem Cells.
Details
HiPSC to i-HCm timeline using Differentiation Medium Kit (017DK)
i-HCm Differentiation Medium Kit, Xeno-Free (017DK)
| Cat. No. | Medium | Abbrev | Vol |
| 017D1-30 | Differentiation 1 | i-HCm DM1 | 30ml |
| 017D2-30 | Differentiation 2 | i-HCm DM2 | 30ml |
| 017D3-100 | Differentiation 3 | i-HCm DM3 | 100ml |
| 019-50 | Selection | i-HCm SM | 50ml |
| 021-30 | Maintenance | i-HCm MM | 30ml |
Store all kit components at 4°C. Stable for 4 weeks at 4ºC.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Cardiomyocyte Differentiation Medium Kit: Differentiation, Selection & Maintenance Media sold together, packaged separately. See Details Tab. | Size: 1 Kit | CAT.#: 017DK | Price: $288.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Cardiomyocyte Maintenance Medium: Maintenance medium for Human iPSC-Derived Cariomyocytes | Size: 50 ml | CAT.#: 021-50 | Price: $68.00 | |
| Human Cardiomyocyte Plating Medium: Use for initial plating of Human iPSC-Derived Cardiomyocytes | Size: 20 ml | CAT.#: 021P-20 | Price: $68.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved HiPSC, from adult Female Dermal Fibroblasts: Frozen Human Induced Pluripotent Stem Cells derived from HDF (1x10^6) (Donor age, gender in Details Tab) | Size: 1 Cryovial | CAT.#: iPS12-10 | Price: $692.00 | |
| Cryopreserved HiPSC, from neonatal Male Dermal Fibroblasts: Frozen Human Induced Pluripotent Stem Cells derived from HDF (1x10^6) (Donor age, gender in details) | Size: 1 Cryovial | CAT.#: iPS11-10 | Price: $692.00 |