Instructions 017DK i-HCm Differentiation Medium Kit
MSDS 017DK

Description

 
 
 
 
 
 
From Skin Cells to Beating Heart Cells!  Dermal fibroblasts (HDF) isolated from human skin, reprogrammed to pluripotent stem cells (HiPSC), then differentiated to cardiomyocytes (i-HCm). Top: In vitro-cultured i-HCm immunofluorescently stained for Alpha Actinin (L) Troponin (R), and nuclei counter-stained for DAPI. Middle: Pulsating heart tissue spheroid (organoid) constructed with Cyfuse Regenova Bio 3D Printer, incorporating neonatal (L) or adult (R) i-HCm & primary cardiac cells. Printed i-HCm derived from adult iPSC exhibit strong beating at 72 pulses per minute while neonatal i-HCm demonstrate weaker, faster beat rate. Bottom: Monolayer-cultured i-HCm beat spontaneously in rhythm.
 
Medium carefully optimized for differentiating Human Induced Pluripotent Stem Cells into iPSC-Derived Cardiomyocytes and maintaining i-HCm.  Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.
 
Use with:
(Click to Enlarge) i-HCm Differentiation Medium Kit is used to derive Cardiomyocytes (i-HCm) from Human Induced Pluripotent Stem Cells.

Details

HiPSC to i-HCm timeline using Differentiation Medium Kit (017DK)
 
i-HCm Differentiation Medium Kit, Xeno-Free (017DK)
Cat. No.MediumAbbrev Vol
017D1-30Differentiation 1i-HCm DM130ml
017D2-30Differentiation 2i-HCm DM230ml
017D3-100Differentiation 3i-HCm DM3100ml
019-50Selectioni-HCm SM50ml
021-30Maintenancei-HCm MM30ml
 
Store all kit components at 4°C. Stable for 4 weeks at 4ºC.
Instructions 017DK i-HCm Differentiation Medium Kit

Format: PDF

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Products

Product Size CAT.# Price Quantity
Human Cardiomyocyte Differentiation Medium Kit: Differentiation, Selection & Maintenance Media sold together, packaged separately. See Details Tab. Size: 1 Kit CAT.#: 017DK Price: $250.00
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Human Cardiomyoycte Plating Medium: Use for initial plating of Human iPSC-Derived Cardiomyocytes Size: 20 ml CAT.#: 021P-20 Price: $59.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cryopreserved HiPSC: Frozen Human Induced Pluripotent Stem Cells derived from HDF (1x10^6) (Donor age, gender in Details Tab) Size: 1 Cryovial CAT.#: iPS12-10 Price: $691.88
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Cryopreserved HiPSC: Frozen Human Induced Pluripotent Stem Cells derived from HDF (1x10^6) (Donor age, gender in details) Size: 1 Cryovial CAT.#: iPS11-10 Price: $691.88
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