Bovine Brain Microvascular Endothelial Cells: BBMVEC. Â Isolated from small cerebral blood vessels.
Bovine Brain Microvascular Endothelial Cells: BBMVEC
Description
Bovine Brain Microvascular Endothelial Cells (BBMVEC)Â provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the blood-brain barrier (BBB).
BBMVEC from Cell Applications, Inc. have been utilized extensively in research, for example to:
- Show that alkalosis activates ERK in aortic, but not brain microvascular endothelial cells
- Study the mechanisms of blood-brain barrier (BBB) penetration by fungal pathogens during invasion
- Demonstrate opposite effects of osteoponin isoforms on angiogenesisin
- Develop an in vitro capillary assay and show VEGF secretion by endothelial cells
- Investigate the mechanisms of accumulation and effects of amyloid deposits on brain vasculature in cerebral amyloid angiopathy
- Improve drug loading and delivery stealth dendrimer carriers
- Report that the BBB breaks down under hypoxic conditions
- In indicate that increased contractility and oxidative stress are involved in development of post-stroke brain edema
- Exhibit blood-brain barrier (BBB) function can result from shear stress, acting through a pathway that upregulates key factors and increases their localization to tight junctions
- Demonstrate that brain microvascular endothelial cells show higher sensitivity to oxidative stress generated by advanced glycation end products due to stronger VEGF expression leading to increased permeability
- Show, along with Bovine Aortic Endothelial Cells, that brain microvasculature is more sensitive to pathogenesis, compared to large vessel endothelia,
- Demonstrate that C-reactive protein (CRP), a cardiovascular risk factor, induces higher oxidative stress in the brain microvasculature
- Support the key role of ROS by showing that activation of antioxidant genes by Nrf2 reduces brain vascular leak from acute high altitude exposure known to induce ROS
- Determine that IL-1β, ZYM, and LTA increase the permeability of the BBB to small ions, while TNF-α and lipopolysaccharide disrupt the endothelial layer integrity to allow passage of larger molecules
- Investigate the role of basolateral environment in modulating BBB by regulating expression and biochemical properties of the tight junction proteins, occludin and ZO-1
- Show that during cerebral ischemia increased expression of TWEAK and Fn14 in the endothelial-astrocyte interface facilitating leukocyte transmigration and recruitment to the ischemic tissue
- Demonstrate that apigenin, a dietary flavonoid, activates Ca2+-activated K+ channels in endothelial cells leading to a hyperpolarization followed by a Ca2+ influx causing increased NO production followed by Akt dephosphorylation
- Develop gene and drug delivery methods for crossing the BBB based on polymer-based nanoparticles or adenovirus or gold nanoparticles modified to be transported via transcytosis pathway
Details
| Tissue | Normal healthy bovine brain microvessels | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Character | DiI-Ac-LDL uptake: Positive | |
| Bioassay | Attach, spread on Attachment Factor-coated surface, proliferate in Growth Med | |
| Cryovial | 500,000 BBMVEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen BBMVEC (B840-05), Gr Med (B819-500), Attchmnt Fctr Soln (123-100), Subcltr Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) | |
| Doublings | At least 12 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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