Human Lung Microvascular Endothelial Cells (HLMVEC) in culture (A). HLMVEC immunolabeled for CD31/PECAM (green); nuclei are visualized with PI (red) (B). HLMVEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit (C,D)
Human Lung Microvascular Endothelial Cells: HLMVEC
Description
Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro. The pulmonary vasculature is of great physiological/pathological significance. It is now recognized to be a tissue composed of metabolically active, functionally responsive cells, that interact with circulating substrates and regulate the composition of systemic arterial blood, affect target organ functions, and contribute to thrombosis, hemostasis and immune reactions, as well as tumor metastasis.
HLMVEC from Cell Applications, Inc. have been used to elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells and inhibition of PKCϵ.
Pre-Screened Human Lung Microvascular Endothelial Cells (S-HLMVEC) are select lots of HLMVEC that have been undergone further characterization. Pre-Screened HLMVEC have been stimulated by VEGF, and assayed for activation of VEGFR2 signaling pathway. More information about pre-screened endothelial cells can be found on the Pre-Screened Endothelial Cell Product Page.
Details
| Tissue | Human lung | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Character | Factor VIII-related Ab, CD31 (PECAM-1), DiI-Ac-LDL uptake. S-HLMVEC are select HLMVEC lots that have been tested positive for VEGFR2 pathway activation following stimulation by VEGF. | |
| Bioassay | Attach, spread on AFS-coated surface, proliferate in Growth Med | |
| Cryovial | 500,000 HLMVEC (3rd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial HLMVEC (540-05), Gr Med (111-500), Attchmnt Fctr Soln (123-100), Sbcltr Rgnt Kit (090K) | |
| Proliferating | Shipped in Tsfr Med, 4th psg (flasks or plates) | |
| Doublings | At least 15 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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