Instructions TF150K
Instructions TF150KS

Description

Transfection, the introduction of nucleic acid into mammalian cells, is a powerful technique used to study in vivo gene function and regulation.  The Cytofect™ Skeletal Muscle Cell Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into a wide variety of smooth muscle cells. This kit contains Cytofect-2 and Media for transfection and culturing cells.  The result has been high transfection efficiency and viability for these traditionally hard-to-transfect cells.
 
#Wells / Rxns
96 / 333
24 / 83
12 / 41
The Kit contains all necessary reagents, media and optimized protocols to carry out successful transfection smooth muscle cells with high efficiency (15-30%) and viability (80%-95%).
Products are for research use only. They are not intended for human, animal, or diagnostic applications. CF1 is manufactured by Targeting Systems.

(Click to Enlarge) Transfection of fetal HSkMC (Cat. No. 150-05f) with a GFP-expression plasmid using CF2.

Details

Kit Contents:
Cytofect -2
Antibiotics-Free Growth Medium
Transfection Medium
 
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
Instructions TF150K

Format: PDF

Download Now
Instructions TF150KS

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cytofect™ Skeletal Muscle Cell Transfection Kit: 83 x 24-Well Rxns Size: 1 Kit CAT.#: TF150K Price: $404.00
-+
Cytofect™ Skeletal Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF150KS Price: $54.00
-+

Related Products

Product Size CAT.# Price Quantity
Cytofect-1 (CF1): Store at -20°C Size: 250 ul CAT.#: 051-250 Price: $152.00
-+
Cytofect-1 (CF1): Store at -20°C Size: 500 ul CAT.#: 051-500 Price: $246.00
-+
Cytofect-2 (CF2) : Store at 4°C Size: 250 ul CAT.#: 052-250 Price: $152.00
-+
Cytofect-2 (CF2) : Store at 4°C Size: 500 ul CAT.#: 052-500 Price: $246.00
-+
Peptide Enhancer (PE): Store at 4°C Size: 250 ul CAT.#: 053-250 Price: $123.00
-+
Peptide Enhancer (PE): Store at 4°C Size: 500 ul CAT.#: 053-500 Price: $205.00
-+
Viral Enhancer (VE): Store at -20°C Size: 250 ul CAT.#: 054-250 Price: $123.00
-+
Viral Enhancer (VE): Store at -20°C Size: 500 ul CAT.#: 054-500 Price: $205.00
-+

Resources/Documents