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Human Lung Fibroblasts: HLF

Quantity

Description

Human Lung Fibroblasts (HLF) provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.

HLF from Cell Applications, Inc. have been utilized in a number of research publications, for example to demonstrate

  • Differential gene expression, cytokines, growth factors and interleukins
  • Receptor activation, inhibition and receptor-interacting protein
  • Cell behavior changes, including synthesis of collagen and other extracellular matrix molecules, as well as actin reorganization, collagen contraction and fibroblast / myofibroblast trans-differentiation.
  • Lung disease, disorders and injury, including hypoxia, tissue rigidity, pulmonary fibrosis and hypertension
  • That unwanted cell growth can be inhibited, and that the healing process can be understood and improved

Details

Tissue
Normal healthy human lung parenchyma
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HLF (1st passage) frozen in in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HLF (506-05a or 506-05f), Gr Med (516-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 12
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

Instructions HLF

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MSDS Cryopreserved Cells

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Resources

Cell Apps Flyer Airway Cells

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5 Important Cell Culture Rules

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Cell Apps Poster Primary Cells

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FAQs

Citations

Extended Products

PRODUCTSIZECAT.#PRICEQUANTITY
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.50 ml040-50$54.00
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution1 Kit, 100 ml090K$69.00
Lung Fibroblast (HLF RNA), Fetal: Total RNA prepared from Human Lung Fibroblasts, fetal10 ug506-R10f$398.00
Lung Fibroblast (HLF RNA), Fetal: Total RNA prepared from Human Lung Fibroblasts, fetal25 ug506-R25f$796.00
Human Lung RNA: Total RNA prepared from human lung tissue50 ug1H40-50$228.00
Human Lung RNA: Total RNA prepared from human lung tissue250 ug1H40-250$851.00
Polyclonal Fibroblast Growth Factor-Receptor 2 Antibody: Polyclonal Fibroblast Growth Factor-Receptor 2 Antibody100 ulCB4365$333.00
Mouse Fibroblast Growth Factor Receptor 1 Antibody: Mouse Fibroblast Growth Factor Receptor 1 Antibody100 ulCP10102$413.00
Mouse Fibroblast Growth Factor Receptor Tyrosine Kinase 4 Antibody: Mouse Fibroblast Growth Factor Receptor Tyrosine Kinase 4 Antibody100 ulCP10103$413.00
Human MIF ELISA Kit: Human Macrophage Migration Inhibitory Factor ELISA Kit96 wellsCL0813$511.00
Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns1 KitTF103K$512.00
Rabbit Macrophage Migration Inhibitory Factor Antibody: Rabbit Macrophage Migration Inhibitory Factor Antibody100 ulCA1052$375.00
Rabbit Fibroblast Growth Factor 8 Antibody: Rabbit Fibroblast Growth Factor 8 Antibody100 ulCA1216$375.00
Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-2120 ugRP1148-20$194.00
Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21100 ugRP1148-100$484.00
Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-211000 ugRP1148-1000$3,175.00
Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-2220 ugRP1068-20$194.00
Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22100 ugRP1068-100$624.00
Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-221000 ugRP1068-1000$5,166.00
Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-425 ugRP1031-25$194.00
Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4100 ugRP1031-100$484.00
Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-41000 ugRP1031-1000$3,175.00
Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic10 ugRP1029-10$81.00
Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic50 ugRP1029-50$194.00
Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic1000 ugRP1029-1000$1,561.00
Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic10 ugRP1028-10$81.00
Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic100 ugRP1028-100$317.00
Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic1000 ugRP1028-1000$1,561.00
Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic10 ugRP1146-10$81.00
Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic100 ugRP1146-100$253.00
Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic1000 ugRP1146-1000$1,561.00
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor20 ugRP1008-20$194.00
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor100 ugRP1008-100$484.00
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor1000 ugRP1008-1000$3,444.00
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 1025 ugRP1127-25$194.00
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10100 ugRP1127-100$484.00
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 101000 ugRP1127-1000$3,175.00
Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor25 ugRP1153-25$194.00
Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor100 ugRP1153-100$484.00
Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor1000 ugRP1153-1000$3,175.00
Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns1 Sample KitTF103KS$68.00
Polyclonal Fibroblast Growth Factor 2 Rabbit Antibody: Polyclonal Fibroblast Growth Factor 2, FGF2 (bFGF), Rabbit Antibody100 ulCA0259$375.00
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free20 ugRP1008AF-20$213.00
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free100 ugRP1008AF-100$533.00
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free1000 ugRP1008AF-1000$3,788.00
Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free10 ugRP1028AF-10$89.00
Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free100 ugRP1028AF-100$347.00
Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free1000 ugRP1028AF-1000$1,717.00
Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free10 ugRP1146AF-10$89.00
Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free100 ugRP1146AF-100$276.00
Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free1000 ugRP1146AF-1000$1,717.00
Size: 50 ugCat.#: 1H40-50Price: $228.00
Size: 250 ugCat.#: 1H40-250Price: $851.00
Size: 96 wellsCat.#: CL0813Price: $511.00
Size: 1 KitCat.#: TF103KPrice: $512.00
Size: 20 ugCat.#: RP1148-20Price: $194.00
Size: 100 ugCat.#: RP1148-100Price: $484.00
Size: 1000 ugCat.#: RP1148-1000Price: $3,175.00
Size: 20 ugCat.#: RP1068-20Price: $194.00
Size: 100 ugCat.#: RP1068-100Price: $624.00
Size: 1000 ugCat.#: RP1068-1000Price: $5,166.00
Size: 25 ugCat.#: RP1031-25Price: $194.00
Size: 100 ugCat.#: RP1031-100Price: $484.00
Size: 1000 ugCat.#: RP1031-1000Price: $3,175.00
Size: 50 ugCat.#: RP1029-50Price: $194.00
Size: 1000 ugCat.#: RP1029-1000Price: $1,561.00
Size: 25 ugCat.#: RP1153-25Price: $194.00
Size: 100 ugCat.#: RP1153-100Price: $484.00
Size: 1000 ugCat.#: RP1153-1000Price: $3,175.00
Size: 1 Sample KitCat.#: TF103KSPrice: $68.00
Size: 1000 ugCat.#: RP1008AF-1000Price: $3,788.00
Size: 100 ugCat.#: RP1028AF-100Price: $347.00
Size: 1000 ugCat.#: RP1028AF-1000Price: $1,717.00
Size: 100 ugCat.#: RP1146AF-100Price: $276.00
Size: 1000 ugCat.#: RP1146AF-1000Price: $1,717.00