MSDS Cryopreserved Cells
Instructions HLF
Cell Apps Flyer Airway Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells

Description

Human Lung Fibroblasts (HLF) provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.
HLF from Cell Applications, Inc. have been utilized in a number of research publications, for example to demonstrate
  • Differential gene expression, cytokines, growth factors and interleukins
  • Receptor activation, inhibition and receptor-interacting protein
  • Cell behavior changes, including synthesis of collagen and other extracellular matrix molecules, as well as actin reorganization, collagen contraction and fibroblast / myofibroblast trans-differentiation.
  • Lung disease, disorders and injury, including hypoxia, tissue rigidity, pulmonary fibrosis and hypertension
  • That unwanted cell growth can be inhibited, and that the healing process can be understood and improved
(Click to Enlarge) Human Lung Fibroblasts: HLF (L) immunolabeled for FSP (green) (R).

Details

Tissue
Normal healthy human lung parenchyma
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HLF (1st passage) frozen in in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HLF (506-05a or 506-05f), Gr Med (116-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 12
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

Instructions HLF

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HLF, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 506K-05a Price: $715.00
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Cryopreserved HLF, Adult: Frozen HLF (5x10^5) Size: 1 Cryovial CAT.#: 506-05a Price: $560.00
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Cryopreserved HLF, Fetal: Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 506K-05f Price: $715.00
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Cryopreserved HLF, Fetal: Frozen HLF (5x10^5) Size: 1 Cryovial CAT.#: 506-05f Price: $560.00
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Proliferating HLF, Adult: Proliferating HLF Size: T-25 Flask CAT.#: 507-25a Price: $560.00
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Proliferating HLF, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 507-75a Price: $764.00
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Proliferating HLF, Adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 507-6Wa Price: $764.00
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Proliferating HLF, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 507-96Wa Price: $893.00
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Proliferating HLF, Fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 507-25f Price: $560.00
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Proliferating HLF, Fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 507-75f Price: $764.00
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Proliferating HLF, Fetal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 507-6Wf Price: $764.00
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Proliferating HLF, Fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 507-96Wf Price: $893.00
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Related Products

Product Size CAT.# Price Quantity
HLF Basal Medium: Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: 515-500 Price: $56.00
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HLF Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 516-500 Price: $97.00
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HLF Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 516K-500 Price: $116.00
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HLF Growth Supplement: Added to Basal Medium to create Growth Medium Size: 15 ml CAT.#: 516-GS Price: $49.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Fibroblast Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF103KS Price: $54.00
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Cytofect™ Fibroblast Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF103K Price: $404.00
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HLF RNA, Fetal: Total RNA prepared from Human Lung Fibroblasts, fetal Size: 10 ug CAT.#: 506-R10f Price: $350.00
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HLF RNA, Fetal: Total RNA prepared from Human Lung Fibroblasts, fetal Size: 25 ug CAT.#: 506-R25f Price: $700.00
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Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free Size: 10 ug CAT.#: RP1028AF-10 Price: $89.00
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Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free Size: 100 ug CAT.#: RP1028AF-100 Price: $347.00
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Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free Size: 1000 ug CAT.#: RP1028AF-1000 Price: $1,717.00
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Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free Size: 10 ug CAT.#: RP1146AF-10 Price: $89.00
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Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free Size: 100 ug CAT.#: RP1146AF-100 Price: $276.00
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Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free Size: 1000 ug CAT.#: RP1146AF-1000 Price: $1,717.00
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Human FGF9 ELISA Kit: Human Fibroblast Growth Factor 9 ELISA Kit Size: 96 Wells CAT.#: CL0348 Price: $457.00
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Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic Size: 10 ug CAT.#: RP1028-10 Price: $81.00
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Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic Size: 100 ug CAT.#: RP1028-100 Price: $317.00
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Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic Size: 1000 ug CAT.#: RP1028-1000 Price: $1,561.00
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Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic Size: 10 ug CAT.#: RP1146-10 Price: $81.00
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Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic Size: 100 ug CAT.#: RP1146-100 Price: $253.00
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Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic Size: 1000 ug CAT.#: RP1146-1000 Price: $1,561.00
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Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic Size: 10 ug CAT.#: RP1029-10 Price: $81.00
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Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic Size: 50 ug CAT.#: RP1029-50 Price: $194.00
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Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic Size: 1000 ug CAT.#: RP1029-1000 Price: $1,561.00
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Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 Size: 20 ug CAT.#: RP1148-20 Price: $194.00
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Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 Size: 100 ug CAT.#: RP1148-100 Price: $484.00
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Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 Size: 1000 ug CAT.#: RP1148-1000 Price: $3,175.00
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Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 Size: 1000 ug CAT.#: RP1068-1000 Price: $5,166.00
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Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 Size: 20 ug CAT.#: RP1068-20 Price: $194.00
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Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 Size: 100 ug CAT.#: RP1068-100 Price: $624.00
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Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 Size: 25 ug CAT.#: RP1031-25 Price: $194.00
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Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 Size: 100 ug CAT.#: RP1031-100 Price: $484.00
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Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 Size: 1000 ug CAT.#: RP1031-1000 Price: $3,175.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $194.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,175.00
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Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 20 ug CAT.#: RP1008AF-20 Price: $213.00
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Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 100 ug CAT.#: RP1008AF-100 Price: $533.00
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Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 1000 ug CAT.#: RP1008AF-1000 Price: $3,788.00
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Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 20 ug CAT.#: RP1008-20 Price: $194.00
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Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 100 ug CAT.#: RP1008-100 Price: $484.00
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Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 1000 ug CAT.#: RP1008-1000 Price: $3,444.00
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Human Lung RNA: Total RNA prepared from human lung tissue Size: 50 ug CAT.#: 1H40-50 Price: $139.00
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Human Lung RNA: Total RNA prepared from human lung tissue Size: 250 ug CAT.#: 1H40-250 Price: $522.00
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Human MIF ELISA Kit: Human Macrophage Migration Inhibitory Factor ELISA Kit Size: 96 wells CAT.#: CL0813 Price: $511.00
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Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor Size: 25 ug CAT.#: RP1153-25 Price: $194.00
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Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor Size: 100 ug CAT.#: RP1153-100 Price: $484.00
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Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor Size: 1000 ug CAT.#: RP1153-1000 Price: $3,175.00
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Mouse Fibroblast Growth Factor Receptor 1 Antibody: Mouse Fibroblast Growth Factor Receptor 1 Antibody Size: 100 ul CAT.#: CP10102 Price: $333.00
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Mouse Fibroblast Growth Factor Receptor Tyrosine Kinase 4 Antibody: Mouse Fibroblast Growth Factor Receptor Tyrosine Kinase 4 Antibody Size: 100 ul CAT.#: CP10103 Price: $333.00
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Polyclonal Fibroblast Growth Factor 2 Rabbit Antibody: Polyclonal Fibroblast Growth Factor 2, FGF2 (bFGF), Rabbit Antibody Size: 100 ul CAT.#: CA0259 Price: $302.00
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Polyclonal Fibroblast Growth Factor 7, KGF, Rabbit Antibody: Polyclonal Fibroblast Growth Factor 7, KGF, Rabbit Antibody Size: 100 ul CAT.#: CA0853 Price: $302.00
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Polyclonal Fibroblast Growth Factor-Receptor 2 Antibody: Polyclonal Fibroblast Growth Factor-Receptor 2 Antibody Size: 100 ul CAT.#: CB4365 Price: $333.00
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Rabbit Fibroblast Growth Factor 8 Antibody: Rabbit Fibroblast Growth Factor 8 Antibody Size: 100 ul CAT.#: CA1216 Price: $302.00
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Rabbit Macrophage Migration Inhibitory Factor Antibody: Rabbit Macrophage Migration Inhibitory Factor Antibody Size: 100 ul CAT.#: CA1052 Price: $302.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Airway Cells

Format: PDF

Downoad Now
5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now

Publications

2015
Peng, H., W. Gao, F. Chong, H. Liu, and J. Zhang. 2015. Semaphorin 4A enhances lung fibrosis through activation of Akt via PlexinD1 receptor. J Biosci, DOI 10.1007/s12038-015-9566-9.
2014
Medarametla, V., S. Festin, C. Sugarragchaa, A. Eng, A. Naqwi, T. Wiedmann and L. Zisman. 2014. PK10453, a nonselective platelet-derived growth factor receptor inhibitor, prevents the progression of pulmonary arterial hypertensionPulm Circ, 4:82-102.
Nyp, M., A. Navarro, M. Rezaiekhaligh, R. Perez, S. mabry, and I. Ekekezie. 2014. TRIP-1 via AKT modulation drives lung fibroblast/myofibroblast trans-differentiation. Respiratory Research, 15:19
Zisman, L. 2014. Non-selective kinase inhibitorsPatent Application US 20150353527 A1.
2013
Ni, J., Z. Dong, W. Han, D. Kondrikov, and Y. Su. 2013. The role of RhoA and cytoskeleton in myofibroblast transformation in hyperoxic lung fibrosis. Free Radical Biology and Medicine. 61:26-39.
2011
Buck, M., and M. Chojkier. 2011. C/EBPβ-Thr217 Phosphorylation Signaling Contributes to the Development of Lung Injury and Fibrosis in Mice. PloS one. 6:e25497.
Zheng, M., D.M. Jones, C. Horzempa, A. Prasad, and P.J. McKeown-Longo. 2011. The first type III domain of fibronectin is associated with the expression of cytokines within the lung tumor microenvironment. Journal of Cancer. 2:478.
2009
Navarro, A., M. Rezaiekhaligh, J.A. Keightley, S.M. Mabry, R.E. Perez, and I.I. Ekekezie. 2009. Higher TRIP-1 level explains diminished collagen contraction ability of fetal versus adult fibroblasts. American Journal of Physiology - Lung Cellular and Molecular Physiology. 296:L928-L935.
Okada, M., K. Suzuki, M. Matsumoto, K. Takada, T. Nakanishi, H. Horikoshi, T. Higuchi, Y. Hosono, M. Nakayama, and F. Ohsuzu. 2009. Effects of angiotensin on the expression of fibrosis-associated cytokines, growth factors, and matrix proteins in human lung fibroblasts. Journal of Clinical Pharmacy and Therapeutics. 34:289-299.
Tofovic, S.P., X. Zhang, E.K. Jackson, H. Zhu, and G. Petrusevska. 2009. 2-methoxyestradiol attenuates bleomycin-induced pulmonary hypertension and fibrosis in estrogen-deficient rats. Vascular pharmacology. 51:190-197.
2008
Tofovic, S.P., X. Zhang, H. Zhu, E.K. Jackson, O. Rafikova, and G. Petrusevska. 2008. 2-Ethoxyestradiol is antimitogenic and attenuates monocrotaline-induced pulmonary hypertension and vascular remodeling. Vascular pharmac. 48:174-183.
2003
Ikeda, J.,, Y. Okada, H., Sakai, and H. Osuga. 2003. Anti-neurodegenerative agents. Patent Applications US 20050261306 A1.
1998
Leung, K.H., V. Pippalla, A. Kreutter, and M. Chandler. 1998. Functional effects of FGF-13 on human lung fibroblasts, dermal microvascular endothelial cells, and aortic smooth muscle cells. Biochem. & Biophys. Research Communic. 250:137-142.