Top: Human Pericardial Fibroblasts (HPcF). Bottom: HPcF stained for fibroblast surface protein (in green) and DAPI (in blue).
Human Pericardial Fibroblasts: HPcF
Human Pericardial Fibroblasts (HPcF) are primary fibroblast cell populations derived from human cardiac tissue.
Description
Human Pericardial Fibroblasts (HPcF) are primary cell populations derived from human cardiac tissue, specifically representing an important cell type within the pericardium. As specialized interstitial cells, they contribute significantly to the structural and biochemical maintenance of the heart.
In vivo, pericardial fibroblasts play a critical role in regulating the extracellular matrix, secreting structural proteins such as collagen and fibronectin, and responding to mechanical stress or injury. While cardiomyocytes provide the contractile force and smooth muscle cells regulate vascular tone, HPcF govern extracellular matrix turnover, cytokine signaling, and tissue remodeling in response to pathological stimuli.
In biomedical research, Human Pericardial Fibroblasts (HPcF) provide an excellent model system to study human heart function and pathophysiology, particularly mechanisms underlying cardiovascular diseases. When cultured in specialized growth media under recommended conditions, HPcF can typically propagate for up to 10 population doublings. Researchers can use these models to explore cellular proliferation, activation, and differentiation pathways, as well as interactions with neighboring cell types such as mesothelial cells and stem cells.
Investigators frequently characterize these cultures by evaluating specific marker expression — such as vimentin and fibroblast surface protein — using immunocytochemistry (ICC) staining, and analyze transcriptional regulation, gene expression, and growth factor signaling using established methods.
Human Pericardial Fibroblasts (HPcF) are the most prevalent cell type in the heart. HPcF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology. HPcF from Cell Applications, Inc. are derived from human heart. HPcF can propagate to 10 population doublings when cultured in Pericardial Fibroblast Growth Medium under the recommended conditions and provide an excellent model system to study heart function and cardiovascular diseases.
Characterization: Positive or fibroblast surface protein by ICC staining.
Details
| Tissue | Normal healthy human heart tissue | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 HPcF (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen HPcF (310-05f), Growth Medium (3111-500), Subcltr Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) | |
| Doublings | At least 16 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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