Human Peripheral Blood CD19+ B Cells: HPBB. Antibody-producing lymphocytes.
Human Peripheral Blood B Cells: HPBB
Human Peripheral Blood B Cells (HPBB) are a diverse cell population of B-lymphocyte subsets isolated from the peripheral blood of healthy donors.
Description
Human Peripheral Blood B Cells (HPBB) are a critical cell type within the adaptive immune system, representing a diverse cell population of B-lymphocyte subsets isolated from the peripheral blood of healthy donors. As circulating B cells, they provide a highly relevant in vitro model to investigate B-cell ontogeny, receptor signaling, and oncology therapeutics. These cells are typically isolated from peripheral blood mononuclear cells (PBMCs), a fraction that also contains T cells, NK cells, and dendritic cells.
B-cell development is a tightly regulated process that occurs primarily within the bone marrow, where hematopoietic stem cells differentiate into pro-B cells, pre-B cells, and finally immature B cells.
- Maturation and Migration: Following initial development, transitional B-cell stages exit the bone marrow to complete their maturation in secondary lymphoid tissue, such as the spleen and lymph nodes.
- Subsets: Within this cell population, the B-cell subset distribution is highly heterogeneous, including naive B cells, which have not yet encountered their cognate antigen, and memory B cells, which provide rapid responses to previously encountered pathogens. These are distinct from other populations like B-1 cells and B-2 cell lineages, as well as specialized subsets like MZ B cells and FO B cells.
The primary function of B cells is to orchestrate the humoral immune response. Upon encountering a specific antigen, B-cell activation is initiated, often requiring crosstalk with T-cell populations.
- Differentiation: Activated B cells undergo clonal expansion and differentiate into plasma cells, which are specialized for high-affinity antibody secretion.
- Signaling: This B-cell response is mediated by the cell surface receptor complex (BCR). The modulation of BCR signaling is essential for regulating B-cell survival, activation, and differentiation.
HPBB cells are indispensable for evaluating how biochemical signals, pollutants, and therapeutic agents modulate the human immune system.
- Immunotoxicology: Researchers use these cells to assess the impact of environmental contaminants on lymphocyte viability, calcium flux, and proliferation.
- Oncology and Therapeutics: In preclinical pipelines, HPBB cells serve as vital non-malignant controls. By testing novel small-molecule inhibitors or immunotherapies — such as those used in B-cell depletion therapy — researchers can establish a therapeutic index to ensure selective cytotoxicity against malignant cells while sparing healthy B cells.
- Clinical Relevance: These models are essential for understanding the mechanics of B-cell depletion and the baseline thresholds of activation in human tissues, providing a benchmark for the next generation of targeted immune-based medicines.
Human Peripheral Blood B Cells (HPBB) provide a useful tool for studying various aspects of pathology and biology of human B lymphocytes in vitro. We isolate HPBB from the blood of healthy donors and cryopreserve them immediately after isolation.
These are the antibody forming cells of the immune system and originate from pluripotent hematopoetic stem cells and differentiate in the fetal liver. In adults, B cells principally originate in the bone marrow. Newly generated, surface Ig-expressing mature B cells leave the bone marrow and circulate into peripheral lymphoid organs.
Details
| Tissue | Normal healthy human peripheral blood |
|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma or virus |
| Character | CD19 positive selection |
| Cryovial | 2,000,000 HPBB frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO |
| Kit | Cryovial Frozen HPBB (6904-20a), Maintenance Medium (615-250) |
| Doublings | N/A - Blood cells don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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