Instructions HPBT
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Peripheral Blood T Cells (HPBT) provide a useful in vitro tool for studying the biology and pathology of CD4+ cells.  HPBT cells play critical roles in the regulation of immune responses, and are responsible for mediating many of the effector mechanisms of the immune system. Increased activity in the field of immunotherapy, coupled with the expense of clinical trials, has led to renewed interest in obtaining high purity human T cells to meet needs for research and development.  Our CD4+ T Cells are isolated from human blood of healthy donors and cryopreserved immediately after isolation.

(Click to Enlarge) Human Peripheral Blood CD4+ T Cells: HPBT

Details

Tissue
Normal healthy human peripheral blood
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
positive selection directly against CD4 
Cryovial
5,000,000 HPBT frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO
Kit
Cryovial Frozen HPBT (6902-50a), Maintenance Medium (615-250)
Cultured Cells
Shipped in Transfer Medium (flasks or plates)
Doublings
N/A - Blood cells don't proliferate in vitro
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HPBT

Format: PDF

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HPBT, Adult: 5x10^6 Cells CD4+ T Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 6902K-50a Price: $431.00
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Cryopreserved HPBT, Adult: Frozen HPBT, CD4+ Size: 1 Cryovial CAT.#: 6902-50a Price: $375.00
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Cultured HPBT, Adult: Cultured CD4+ Cells in Transfer Medium Size: T-25 Flask CAT.#: 6903-25a Price: $375.00
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Cultured HPBT, Adult: Cultured CD4+ Cells in Transfer Medium Size: T-75 Flask CAT.#: 6903-75a Price: $565.00
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Cultured HPBT, Adult: Cultured CD4+ Cells in Transfer Medium Size: 24 Well CAT.#: 6903-24Wa Price: $565.00
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Cultured HPBT, Adult: Cultured CD4+ Cells in Transfer Medium Size: 96 Well CAT.#: 6903-96Wa Price: $685.00
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Related Products

Product Size CAT.# Price Quantity
Human Blood Cell Basal Medium: Basal medium (contains no growth supplement).  Add Supplement before use. Size: 250 ml CAT.#: 614-250 Price: $38.00
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Human Blood Cell Culture Medium: Optimized and ready for use. Size: 250 ml CAT.#: 615-250 Price: $66.00
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Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately Size: Yields 250 ml CAT.#: 615K-250 Price: $76.00
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Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium Size: 40 ml CAT.#: 615-CS Price: $40.00
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-LFA-1a: Rabbit LFA-1a/CD11a/ITGAL Antibody Size: 100 ul CAT.#: CG1754 Price: $295.00
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells Size: 50 ml CAT.#: 040-50BC Price: $65.00
-+
Human CD40 Ligand (CD40L): Human Cluster of Differentiation 40 Ligand Size: 10 ug CAT.#: RP1025-10 Price: $86.00
-+
Human CD40 Ligand (CD40L): Human Cluster of Differentiation 40 Ligand Size: 100 ug CAT.#: RP1025-100 Price: $317.00
-+
Human CD40 Ligand (CD40L): Human Cluster of Differentiation 40 Ligand Size: 1000 ug CAT.#: RP1025-1000 Price: $1,561.00
-+
Human CD40/TNFRSF5 ELISA Kit: Human Cluster of Differentiation 40/Tumor necrosis factor ligand superfamily member 5 ELISA Kit Size: 96 Wells CAT.#: CL0702 Price: $511.00
-+
Human CD40L ELISA Kit: Human Cluster of Differentiation 40 Ligand ELISA Kit Size: 96 Wells CAT.#: CL0573 Price: $533.00
-+
Human CD40L, Animal-Free: Human Cluster of Differentiation 40 Ligand, Animal-Free Size: 10 ug CAT.#: RP1025AF-10 Price: $95.00
-+
Human CD40L, Animal-Free: Human Cluster of Differentiation 40 Ligand, Animal-Free Size: 100 ug CAT.#: RP1025AF-100 Price: $347.00
-+
Human CD40L, Animal-Free: Human Cluster of Differentiation 40 Ligand, Animal-Free Size: 1000 ug CAT.#: RP1025AF-1000 Price: $1,717.00
-+
Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 10 ug CAT.#: RP1021AF-10 Price: $213.00
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Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 100 ug CAT.#: RP1021AF-100 Price: $533.00
-+
Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 1000 ug CAT.#: RP1021AF-1000 Price: $4,499.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 10 ug CAT.#: RP1021-10 Price: $194.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 100 ug CAT.#: RP1021-100 Price: $484.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 1000 ug CAT.#: RP1021-1000 Price: $4,090.00
-+
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 20 ug CAT.#: RP1008AF-20 Price: $213.00
-+
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 100 ug CAT.#: RP1008AF-100 Price: $533.00
-+
Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 1000 ug CAT.#: RP1008AF-1000 Price: $3,788.00
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Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 20 ug CAT.#: RP1008-20 Price: $194.00
-+
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 100 ug CAT.#: RP1008-100 Price: $484.00
-+
Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 1000 ug CAT.#: RP1008-1000 Price: $3,444.00
-+
Human IFN gamma ELISA Kit: Human Interferon gamma ELISA Kit Size: 96 Wells CAT.#: CL0373 Price: $554.00
-+
Human IFN-g, Animal-Free: Human Interferon-gamma, Animal-Free Size: 20 ug CAT.#: RP1077AF-20 Price: $95.00
-+
Human IFN-g, Animal-Free: Human Interferon-gamma, Animal-Free Size: 100 ug CAT.#: RP1077AF-100 Price: $213.00
-+
Human IFN-g, Animal-Free: Human Interferon-gamma, Animal-Free Size: 1000 ug CAT.#: RP1077AF-1000 Price: $1,302.00
-+
Human IL-2 ELISA Kit: Human Interleukin-2 ELISA Kit Size: 96 Wells CAT.#: CL0397 Price: $484.00
-+
Human IL-2, Animal-Free: Human Interleukin-2, Animal-Free Size: 10 ug CAT.#: RP1012AF-10 Price: $95.00
-+
Human IL-2, Animal-Free: Human Interleukin-2, Animal-Free Size: 100 ug CAT.#: RP1012AF-100 Price: $347.00
-+
Human IL-2, Animal-Free: Human Interleukin-2, Animal-Free Size: 1000 ug CAT.#: RP1012AF-1000 Price: $923.00
-+
Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit Size: 96 Wells CAT.#: CL0410 Price: $517.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 100 ug CAT.#: RP1010AF-100 Price: $533.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 1000 ug CAT.#: RP1010AF-1000 Price: $3,492.00
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Human IL-6, Animal Free: Human Interleukin-6, Animal-Free Size: 20 ug CAT.#: RP1010AF-20 Price: $213.00
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Human Interferon Inducible T cell Alpha Chemokine (I-TAC / CXCL11): Human Interferon-Inducible T cell alpha Chemokine Size: 100 ug CAT.#: RP1128-100 Price: $484.00
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Human Interferon Inducible T cell Alpha Chemokine (I-TAC / CXCL11): Human Interferon-inducible T cell alpha Chemokine Size: 1000 ug CAT.#: RP1128-1000 Price: $3,175.00
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Human Interferon Inducible T cell Alpha Chemokine (I-TAC / CXCL11): Human Interferon-Inducible T cell alpha Chemokine Size: 20 ug CAT.#: RP1128-20 Price: $194.00
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Human Interferon-Gamma (IFN-gamma): Human Interferon-gamma Size: 20 ug CAT.#: RP1077-20 Price: $86.00
-+
Human Interferon-Gamma (IFN-gamma): Human Interferon-gamma Size: 100 ug CAT.#: RP1077-100 Price: $194.00
-+
Human Interferon-Gamma (IFN-gamma): Human Interferon-gamma Size: 1000 ug CAT.#: RP1077-1000 Price: $1,184.00
-+
Human Interleukin-2 (IL-2): Human Interleukin-2 Size: 10 ug CAT.#: RP1012-10 Price: $86.00
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Human Interleukin-2 (IL-2): Human Interleukin-2 Size: 100 ug CAT.#: RP1012-100 Price: $317.00
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Human Interleukin-2 (IL-2): Human Interleukin-2 Size: 1000 ug CAT.#: RP1012-1000 Price: $839.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 20 ug CAT.#: RP1010-20 Price: $194.00
-+
Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 100 ug CAT.#: RP1010-100 Price: $484.00
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Human Interleukin-6 (IL-6): Human Interleukin-6 Size: 1000 ug CAT.#: RP1010-1000 Price: $3,175.00
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Human L-Selectin ELISA Kit: Human L-Selectin ELISA Kit Size: 96 wells CAT.#: CL0503 Price: $500.00
-+
Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha Size: 20 ug CAT.#: RP1147-20 Price: $194.00
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Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha Size: 100 ug CAT.#: RP1147-100 Price: $484.00
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Human Macrophage Inflammatory Protein-3 Alpha (MIP-3 alpha/CCL20): Human Macrophage Inflammatory Protein-3 alpha Size: 1000 ug CAT.#: RP1147-1000 Price: $3,175.00
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Human MIP-3a ELISA Kit: Human Macrophage Inflammatory Protein-3 alpha ELISA Kit Size: 96 Wells CAT.#: CL0453 Price: $495.00
-+
Human Rantes ELISA Kit: Human Regulated Upon Activation, Normal T Cell Expressed and Secreted ELISA Kit Size: 96 Wells CAT.#: CL0494 Price: $500.00
-+
Human Regulated upon Activation, Normal T Cell Expressed & Secreted (RANTES): Human Regulated on Activation Normal T Cell Expressed & Secreted Size: 20 ug CAT.#: RP1138-20 Price: $194.00
-+
Human Regulated upon Activation, Normal T Cell Expressed & Secreted (RANTES): Human Regulated on Activation Normal T Cell Expressed & Secreted Size: 100 ug CAT.#: RP1138-100 Price: $484.00
-+
Human Regulated upon Activation, Normal T Cell Expressed & Secreted (RANTES): Human Regulated on Activation Normal T Cell Expressed & Secreted Size: 1000 ug CAT.#: RP1138-1000 Price: $3,175.00
-+
Human TNF-alpha ELISA Kit: Human Tumor Necrosis Factor alpha ELISA Kit Size: 96 Wells CAT.#: CL0525 Price: $517.00
-+
Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free Size: 10 ug CAT.#: RP1111AF-10 Price: $85.00
-+
Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free Size: 100 ug CAT.#: RP1111AF-100 Price: $347.00
-+
Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free Size: 1000 ug CAT.#: RP1111AF-1000 Price: $3,492.00
-+
Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Factor-alpha Size: 10 ug CAT.#: RP1111-10 Price: $78.00
-+
Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Factor-alpha Size: 1000 ug CAT.#: RP1111-1000 Price: $3,175.00
-+
Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Facto-alpha Size: 100 ug CAT.#: RP1111-100 Price: $317.00
-+
Mouse Cluster of Differentiation 4 Antibody: Mouse Cluster of Differentiation 4 Antibody Size: 100 ul CAT.#: CP10035 Price: $302.00
-+
Mouse Cluster of Differentiation 40 Antibody: Mouse Cluster of Differentiation 40 Antibody Size: 100 ul CAT.#: CP10045 Price: $333.00
-+
Mouse Cluster of Differentiation 45 Antibody: Mouse Cluster of Differentiation 45 Antibody Size: 100 ul CAT.#: CP10047 Price: $302.00
-+
Mouse Interleukin-2 Antibody: Mouse Interleukin-2 Antibody Size: 100 ul CAT.#: CP10324 Price: $302.00
-+
Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody Size: 100 ul CAT.#: CP10325 Price: $302.00
-+
Polyclonal C-C Chemokine Receptor 5 Rabbit Antibody: Polyclonal C-C Chemokine Receptor 5 Rabbit Antibody Size: 100 ul CAT.#: CA0707 Price: $302.00
-+
Polyclonal CD40 Ligand Antibody: Polyclonal CD40 Ligand Antibody Size: 100 ul CAT.#: CA1020 Price: $302.00
-+
Rabbit CCR7 Antibody: Rabbit CCR7 Antibody Size: 100 ul CAT.#: CG1678 Price: $384.00
-+
Rabbit CD40 Antibody: Rabbit CD40 Antibody Size: 100 ul CAT.#: CA1019 Price: $302.00
-+
Rabbit Tumor Necrosis Factor Receptor 1 Antibody: Rabbit Tumor Necrosis Factor Receptor 1 Antibody Size: 100 ul CAT.#: CG1602 Price: $384.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Cardiovascular Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2016
Nishimura, M., T. Wada, C. Shirakawa, and M. Doi. 2016. Trehalose and Dextran-Containing Solution for Transplanting Mammalian Cells. Patent Application US 20160120170 A1.
Teramura, Y., S. Asif, K. Ekdahl, E. Gustafson and B. Nilsson. 2016. Cell adhesion induced using surface modification with cell-penetrating peptide-conjugated PEG-lipid: A new cell glue for 3D cell-based structuresACS Appl Mater Interfaces, DOI: 10.1021/acsami.6b14584.