Human Peripheral Blood CD4+ T Cells: HPBT
Human Peripheral Blood T Cells: HPBT
Human Peripheral Blood T Cells (HPBT) are a specialized population of T lymphocytes isolated from the peripheral blood of healthy donors.
Description
Human Peripheral Blood T Cells (HPBT) are a specialized population of T lymphocytes isolated from the peripheral blood of healthy donors. As central effectors of cell-mediated adaptive immunity, these cells are essential for orchestrating the immune response, executing targeted cytotoxicity against tumor cells, and establishing long-term memory. Within peripheral blood mononuclear cells (PBMCs), HPBT are distinct from other human mononuclear cells such as B cells, NK cells, and monocyte populations. While often studied as pan-T cells, they comprise diverse subsets including αβ T cells and γδ T cells — including Vγ9Vδ2 T cells (a major γδ T-cell subset in humans) and other γδ T-cell subsets.
T-cell development follows a highly regulated program, beginning with progenitors in the bone marrow before migrating to the thymus for maturation.
- Selection and Diversity: Within the thymus, T cells undergo somatic recombination of their TCR genes. They undergo positive selection (MHC restriction) and negative selection (deletion of self-reactive clones) to ensure self-tolerance while maintaining functional antigen recognition.
- Peripheral Circulation: Mature T cells exit the thymus to circulate through the blood and secondary lymphoid organs. This T-cell population is heterogeneous, with each T-cell subset — ranging from CD4 T cells to effector memory T cells — defined by its unique developmental history and functional potential.
T cells act as the primary coordinators of the adaptive immune system, utilizing their TCRs to recognize peptide antigens presented by MHC class I (CD8+ T cells) or MHC class II (CD4 T cells).
- Effector Functions: Upon activation, T cells differentiate into effector lineages that execute specific immune functions, such as cytokine secretion (e.g., by CD4 T cells) or direct target cell lysis (e.g., by CD8+ T cells).
- Specialized Subsets: Beyond the classical αβ T-cell lineages, human γδ T cells represent a potent, innate-like subset known for rapid responses to stress ligands. These cells are particularly relevant in the context of infectious disease and host defense.
HPBT are indispensable tools for advancing immunology, medicine, and stem cell technologies.
- Cancer Immunotherapy: HPBT are the primary substrate for cancer immunotherapy, including the development of CAR-T and TCR-engineered therapies. By utilizing cells from a patient or healthy donor, researchers can optimize expansion protocols and refine the kinetics of tumor destruction for a clinical trial.
- Disease Modeling: These cells are critical for investigating infectious disease pathogenesis. For example, CD4 T cells are the standard model for studying HIV-1 latency, and HPBT are used to evaluate immune interactions during chronic infections like hepatitis B.
- Biosensors and Diagnostics: Due to their sensitivity, T cells are increasingly integrated into biosensor platforms to measure early activation markers. These systems serve as high-fidelity tools for detecting minute concentrations of bacterial or viral threats.
- Comparative Analysis: In experimental settings, HPBT are often used alongside cell lines to provide a non-malignant, primary human baseline, ensuring that therapeutic findings are representative of an actual human immune response.
Human Peripheral Blood T Cells (HPBT) provide a useful in vitro tool for studying the biology and pathology of CD4+ cells.
HPBT cells play critical roles in the regulation of immune responses, and are responsible for mediating many of the effector mechanisms of the immune system.
Increased activity in the field of immunotherapy, coupled with the expense of clinical trials, has led to renewed interest in obtaining high purity human T cells to meet needs for research and development. Our CD4+ T Cells are isolated from human blood of healthy donors and cryopreserved immediately after isolation.
Details
| Tissue | Normal healthy human peripheral blood | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Character | positive selection directly against CD4 | |
| Cryovial | 5,000,000 HPBT frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO | |
| Kit | Cryovial Frozen HPBT (6902-50a), Maintenance Medium (615-250) | |
| Doublings | N/A – Blood cells don’t proliferate in vitro | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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