Top: Porcine Cardiac Valve Interstitial Cells (PCVIC). Bottom left: PCVIC stained for vimentin (in green) and DAPI (in blue). Bottom right: PCVIC stained for α-smooth muscle actin (in green) and DAPI (in blue).
Porcine Cardiac Valve Interstitial Cells: PCVIC
Description
Porcine Cardiac Valve Interstitial Cells (PCVIC) are a heterogeneous population of specific cell types responsible for maintaining the structural integrity and normal functioning of the valve. Primary culture of valve interstitial cells is useful in exploring cellular and molecular pathogenesis of heart valve disease and discovering new therapeutic targets for cardiovascular diseases.
PCVIC from Cell Applications, Inc. are derived from porcine heart and cultured in Porcine Cardiac Valve Interstitial Cell Growth Medium. PCVIC can propagate to 10 population doublings when cultured under the recommended conditions and provide an excellent model system to study heart valve function and pathobiology.
Characterization: Positive for α-smooth muscle actin and vimentin by ICC staining.
Details
| Tissue | Normal healthy porcine heart | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Character | Positive for α-smooth muscle actin and vimentin | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 PCVIC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen PCVICÂ (P370-05n), Growth Medium (P3113-500), Subculture Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) | |
| Doublings | At least 15 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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