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Cytofect™ Chondrocyte Transfection Kit

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Description

Transfection, the introduction of DNA into cells, is a powerful technique used to study in vivo gene function and regulation. Cytofect™ Chondrocyte Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into chondrocytes. This kit contains Cytofect-2, Viral Enhancer* and Media for transfection and culturing cells. Viral Enhancer complexes with DNA and Cytofect-2 and escorts the tranfection complex to the nucleus. This results in high transfection efficiency and viability for these traditionally hard-to-transfect primary cells.

#Wells / Rxns

96 / 875

24 / 175

12 / 86

The Kit contains all necessary reagents, media and optimized protocols to carry out successful transfection of Human Chondrocytes with good efficiency (35%) and viability (80%-95%).

Products are for research use only. They are not intended for human, animal, or diagnostic applications. Viral Enhancer (VE) is a replication-deficient, Adenovirus-derived formulation that does not contain replication competent virus. TR1 and VE are manufactured by Targeting Systems.

Details

Kit Contents: Cytofect -2
Viral Enhancer (VE)
Antibiotics-Free Growth Medium
Transfection Medium
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
Instructions TF402K

Format: PDF

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Instructions TF402KS

Format: PDF

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