Human Mammary Epithelial Cell Media. Contains growth factors, cytokines and supplements optimized for these breast tissue cells.
Human Mammary Epithelial Cell Media
HMEpC Growth Medium: All-in-one ready-to-use
Type: Growth Medium
Size: 500 ml
Price: $143.00
Description
Human Mammary Epithelial Cell Media are specialized cell culture formulations designed to support the isolation, proliferation, and functional maintenance of primary human mammary epithelial cells (HMEpC) in vitro. In normal breast tissue, the mammary epithelium forms a ductal and alveolar bilayer composed of luminal epithelial cells and basal/myoepithelial cells, maintained by lineage-restricted stem and progenitor cell populations. Formulated under serum-free or defined conditions, these media help preserve primary HMEpC morphology, lineage identity, and proliferative capacity across serial passages.
In cell biology and breast cancer research, HMEpC culture systems provide essential models for investigating normal gland development, epithelial lineage hierarchy, and mechanisms of breast cancer initiation and progression. Human Mammary Epithelial Cell Media support experiments examining epithelial cell proliferation, cellular senescence, DNA damage responses, and epithelial-to-mesenchymal transition (EMT). These culture conditions are also used in patient-derived and cancer-focused studies, including work related to cancer stem cell (CSC) properties and how genetic alterations and microenvironmental cues can drive tumorigenesis.
HMEpC cultures grown in these media are commonly characterized using canonical luminal and basal markers, including Cytokeratins (CK8/18 for luminal cells and CK5/14 for basal/myoepithelial lineages), EpCAM and E-cadherin, and basal-associated markers such as CD44 (with epithelial differentiation and “stemness” assessed by marker panels and functional readouts). Depending on the research goals, HMEpC can be studied using mammosphere formation assays, 3D Matrigel acinar morphogenesis, EMT marker analyses, and co-culture experiments with stromal fibroblasts and other components to better model tissue-level interactions.
Human Mammary Epithelial Cell Media provide a standardized, high-performance culture platform for primary human breast epithelial cells. By offering defined nutritional support and reducing experimental variability, these media enable reproducible data generation across mammary gland biology, breast cancer research, and evaluation of compounds and therapeutic strategies.
Medium carefully optimized for the characteristics and requirements unique to Human Mammary Epithelial Cells. Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data. As with the majority of our epithelial cell media, this product contains no serum.
Use with:
Komatsu, N., Y. Fujita, M. Matsuda, and K. Aoki. 2015. mTORC1 upregulation via ERK-dependant gene expression change confers intrinsic resistance to MEK inhibitors in oncogenic KRas-mutant cancer cells. Oncogene, 23 Feb. (Mammary Epithelial Cell Basal Medium & Supplements) – See more at: Cell Applications Citations
Cell Applications’ Human Mammary Epithelial Cell Growth Media contains Bovine Pituitary Extract (BPE) as a core component. See the BPE video below:
Details
CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions. The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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