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Human Skeletal Muscle Cell Media
Human Skeletal Muscle Cell Media, for in vitro, sterile culture of cells isolated from striated muscle tissue.  HSkMC contain actin and myosin, which run the length of the muscle fiber and are important in muscle contraction.

Human Skeletal Muscle Cell Media

HSkMC Growth Medium: All-in-one ready-to-use

Type: Growth Medium
Size: 500 ml

CAT. #: 151-500

Price: $168.00

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Description

Human Skeletal Muscle Cell Media is a specialized growth medium formulated to support primary human skeletal muscle cells during cell culture, including myoblasts derived from satellite cells. In the body, satellite cells reside between the basal lamina and sarcolemma of skeletal muscle fibers, functioning as muscle stem cells that activate following injury, proliferate as mononucleated myoblasts, and fuse to form multinucleated muscle fibers. Cultured human skeletal muscle cells replicate this myogenic progression—transitioning from proliferating myoblasts into multinucleated myotubes—providing a primary cell model for studying muscle development, repair, and neuromuscular biology.

In vitro, Human Skeletal Muscle Cell Media supports the expansion and differentiation of primary skeletal muscle cells under controlled culture conditions, helping maintain cell viability through routine passaging while supporting myotube formation. This makes the media essential for experimental workflows where primary human cell culture is required over immortalized cell lines. For differentiation and fusion assays, the medium enables a predictable transition to multinucleated myotubes, supporting functional studies that rely on muscle-specific phenotypes, morphology, and differentiation state.

Human Skeletal Muscle Cell Media is widely used in translational research, including regenerative medicine and in vitro disease modeling of muscle injury and muscle cell stress responses. In oncology and metabolic research, primary myoblast cultures are used to study cancer cachexia, rhabdomyosarcoma, and tumor-mediated muscle wasting, where contractile differentiation and metabolic stress pathways can be assessed in a human skeletal muscle context. Researchers also use the medium in analytical assays and preclinical drug discovery workflows to evaluate how candidate compounds affect myoblast viability, cell stress responses, and myotube fusion.

Finally, Human Skeletal Muscle Cell Media can be integrated with molecular tools and functional assays to measure growth factor responsiveness and differentiation status, supporting downstream readouts such as gene expression analysis and protein characterization. Standardized media formulations help ensure reproducibility across experiments, providing a consistent culture environment from initial myoblast expansion through terminal differentiation into multinucleated myotubes.

Medium carefully optimized for the characteristics and requirements unique to human muscle cells.  Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.

Details

CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions.  The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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