Mouse Striatal Neurons (MStN) culture on day 7. A: Phase contrast, 100x. B: Immunostaining for β-III Tubulin (green), GFAP (red), DAPI (blue), 100x.
Mouse Striatal Neurons: MStN
Mouse Striatal Neurons (MStN) are primary neural cells derived from the embryonic striatum — a subcortical component of the forebrain and a core structure of the basal ganglia.
Description
Mouse Striatal Neurons (MStN) are primary neural cells derived from the embryonic striatum — a subcortical component of the forebrain and a core structure of the basal ganglia. When cultured under recommended conditions, these neurons arborize and form complex neurite networks, extending primary dendrites with dendritic spines that can form synapse-like contacts. These primary neostriatal neurons stain positive for classical neuronal markers such as class III beta-tubulin.
The striatum (including both the dorsal striatum and ventral striatum, such as the nucleus accumbens) receives and processes critical input signals from the cerebral cortex (cortical neurons). Within this complex striatal region, robust interactions among cholinergic (cholinergic interneuron, other interneurons), dopaminergic (dopamine, dopamine d1 receptor, activation), and GABAergic neurotransmitter systems can occur. The vast majority of these projection populations comprise striatal medium spiny neurons (or striatal msns, striatal medium spiny neuron, which are distinct from interneurons and project to downstream targets like the globus pallidus and substantia nigra in vivo). Together, these cell type networks play a key role in voluntary movement, reward-based habit learning, addiction, and procedural memory formation.
Studies investigating striatal function utilize primary cultures to model complex neurobiology and neurodegenerative or neuropsychiatric disease states. Researchers can evaluate electrophysiological properties—including resting membrane potential, evoked action potential firing, and responses to current injection—as well as measure changes in gene expression, specific genes, and translated proteins through downstream molecular assays.
Targeted neuropharmacological investigations using a mouse model or in vitro platforms enable examination of receptor kinetics and local cell responses. Cultures of striatal neuron models can be applied across a wide range of analytical workflows, including toxicity tests, immunostaining, live-cell imaging, and electrophysiology — with optional co-culture with glia depending on the specific prep — providing a robust experimental platform for molecular neuroscience studies.
Mouse Striatal Neurons (MStN) are derived from striatum of day 18 embryonic CD1 mouse brain. When cultured under the recommended conditions, MStN arborize and form complex neurite network in one week. MStN Stain positive for β III-Tubulin.
The striatum is a subcortical part of the forebrain. It receives and processes input signals from different parts of the cerebral cortex. With its robust interactions among cholinergic, dopaminergic, and GABAergic neurotransmitter systems, striatum plays a key role in voluntary movement, reward-based habit learning, addiction, procedural memory formation, and cognitive dysfunctions in Parkinson’s disease. Studies using striatal neurons will allow a better understanding of the striatal-related functions and diseases. Cultures of striatal neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.
Details
| Tissue | Normal healthy mouse brain | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Character | Positive for β-III Tubulin | |
| Bioassay | Plate on Poly-D-Lysine coated surface, Arborize to form neurite network in Culture Med | |
| Cryovial | 1M MStN Cryopreserved in Neuron Freezing Medium | |
| Kit | Cryovial frzn MStN (M8812N-10), Neuron Ctng Soln I (027-05), Plating (M817P-10) & Cultr Med (M817-100) | |
| Doublings | N/A – Neurons don’t proliferate in vitro | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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