Top: Rat Cardiomyocytes (RCm) immunolabeled for desmin, large field of view showing nearly 100% desmin-positive cardiomyocytes, 20x. Middle: RCm stained for desmin. Bottom: Detailed view of desmin (green) and actin (red) striations within RCm; nuclei stained with DAPI (blue), 40x.
Rat Cardiomyocytes: RCm
Description
Rat Cardiomyocytes (RCm) are primary cells derived from normal rat heart tissue and plated on inserts or plates immediately after primary isolation. RCm retain their physiological functions (including beating) and provide a useful in vitro model to study cardiotoxicity.
RCm from Cell Applications, Inc. have been used to investigate mechanisms of mitochondrial and cardiovascular toxicity exhibited by anti-cancer drugs targeting angiogenesis. Antipsychotic drugs, thioridazine and mesoridazine, were shown to inhibit gap junction communication and affect the beating of RCm, explaining the cardiac side-effects observed in some patients. Clostridium difficile toxin B was shown to disrupt coordinated contractility and rhythmicity in RCm, but the damage was prevented by a caspase-3 inhibitor.
Additionally, RCm were used to demonstrate that Mas receptor, a GPCR activates phospholipase C and causes inositol phosphate accumulation, contributing to cardiac ischemia-reperfusion injury. The same system was then used to screen for agonists and inverse agonists for Mas. Inhibiting Mas function provided cardioprotective effects in mice and in isolated rat hearts. RCm were also used to develop a new probe for positron emission tomography that allows for more effective cardiac imaging.
***FOR SHIPMENT THE FOLLOWING MONDAY, PLEASE PLACE RCm ORDERS BY TUESDAY 12pm PST the week prior.***
Details
| Tissue | Normal healthy neonatal rat heart | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Bioassay | Attaches on FBS-coated culture ware, Remains viable 2-4 days in Cltr Med | |
| Cryovial | N/A | |
| Kit | N/A | |
| Cultured | Shipped w/in 3 days after plating in Maint Med (flasks or plates) | |
| Doublings | N/A | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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