Rat Osteoblasts: ROb (L) von Kossa stained (R). Bone forming cells.
Rat Osteoblasts: ROb
Rat Osteoblasts (ROb) are primary bone cells from rats.
Description
Rat Osteoblasts (ROb) are primary, lineage-specific bone cells from rats. Functioning as the primary bone forming cells of the skeletal system, these highly specialized mesenchymal cells are dedicated to synthesizing the organic extracellular matrix and orchestrating structural calcification.
In the living organism, these cells are located within rat bone, specifically lining the surface layers of primary bone tissue such as the periosteum and endosteum. They reside precisely at sites of active skeletal modeling, remodeling, and structural repair.
The fundamental biological role of these cells is driving bone formation and bone mineralization to preserve structural integrity. During this process, they exhibit high alkaline phosphatase activity and initiate the gene expression of critical bone matrix proteins, notably osteocalcin. To maintain overall bone health, they also secrete coupling factors that modulate the counter-balancing activity of osteoclasts, the cells responsible for bone resorption, ensuring skeletal mass remains stable.
For research use, ROb provide an invaluable, highly translatable in vitro model system documented across extensive literature sources. They are widely utilized to investigate the molecular mechanisms governing cellular proliferation, osteoblastic differentiation, and osteogenic differentiation. By simulating cellular imbalances that lead to accelerated bone loss, researchers use them to model metabolic diseases like osteoporosis and evaluate therapeutic interventions intended for the human skeletal system. In the laboratory, cultivating these cells requires media supplemented with fetal bovine serum to support metabolic demand. Maintaining phenotypic integrity requires strict adherence to aseptic operation protocols. While broad-spectrum antibiotics like penicillin and streptomycin are standard to prevent routine bacterial overgrowth, rigorous quality control is mandatory to ensure the cell lines remain entirely free from disruptive, fastidious contaminants like mycoplasma and fungi.
Rat Osteoblasts (ROb) provide a model system to study matrix mineralization, effects of inflammatory mediators and bone morphogenic proteins, physiological control of bone remodeling and regulation of bone metabolism.
Characterization: Positive for bone mineralization
Details
| Tissue | Normal healthy rat bone | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Characther | Bone mineralization (Kossa stain) | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 ROb (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen ROb (R406-05a), Growth Med (R417-500), Subculture Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, psg 2, flasks or plates | |
| Doublings | At least 10 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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