MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions HHDF
Description
Human Hepatic Duct Fibroblasts (HHDF) are unique cell type that play an important role in liver homeostasis and maintenance. Primary culture of Hepatic Duct Fibroblasts is suitable for many cell-based assays including toxicity, drug screening and metabolism and is a valuable tool to study liver function, physiology and related liver diseases.
Human Hepatic Duct Fibroblasts: HHDF, Left: phase contrast, Right: HHDF immunostained for fibroblast surface protein (green) and nuclei are stained with DAPI.
Details
| Tissue | Normal healthy human liver tissue |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 HHDF (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen HHDF (702-05f), Growth Medium (7111-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) |
| Doublings | At least 15 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved HHDF, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics). | Size: 1 Kit | CAT.#: 702K-05f | Price: $885.00 | |
| Cryopreserved HHDF, fetal: Frozen HHF (5x10^5) | Size: 1 Cryovial | CAT.#: 702-05f | Price: $725.00 | |
| Proliferating HHDF, fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 703-25f | Price: $725.00 | |
| Proliferating HHDF, fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 703-75f | Price: $915.00 | |
| Proliferating HHDF, fetal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 703-24Wf | Price: $915.00 | |
| Proliferating HHDF, fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 703-96Wf | Price: $1,035.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Hepatic Fibroblast Basal Medium : Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 7110-500 | Price: $57.00 | |
| Human Hepatic Fibroblast Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 7111-500 | Price: $115.00 | |
| Human Hepatic Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 7111K-500 | Price: $126.00 | |
| Human Hepatic Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: 7111-GS | Price: $58.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $139.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $36.00 | |
| Cytofect™ Fibroblast Transfection Kit: 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF103K | Price: $404.00 | |
| Cytofect™ Fibroblast Transfection Kit: 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF103KS | Price: $54.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $55.00 |