MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions HHSC (2)

Description

Hepatic Stellate Cells (HHSC) are one of the major cell type that reside between hepatocytes and blood vessels in the liver. HHSCs play an important role in liver homeostasis, maintenance and regeneration including retinol metabolism. Activation of stellate cells in disease state leads to liver fibrosis, hepatitis B & C, fatty liver disease, diabetes and other chronic diseases. Hepatic Stellate Cells are primary cells derived from human liver and cultured in optimized Hepatic Stellate Cells Growth Medium. Primary culture of Hepatic Stellate Cells is suitable for many cell-based assays including toxicity, drug screening and metabolism and is a valuable tool to study liver function, physiology and liver diseases
 

Characterization: Positive for α-smooth muscle actin and fibronectin by ICC staining.

 

Human Hepatic Stellate Cells: HHSC Left: HHSC immunolabeled for α-smooth muscle actin (green) Right: HHSC immunostained for fibronectin (green) nuclei are stained with DAPI.

Details

TissueNormal healthy human liver tissue
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 HHSC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
KitCryovial frozen HHSC (782-05f), Growth Medium (7117-500), Subcltr Rgnt Kit (090K)
ProliferatingShipped in Gr Med, 2nd psg (flasks or plates)
DoublingsAt least 15
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HHSC Total Kit: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 782K-05f Price: $895.00
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Cryopreserved HHSC, fetal: Frozen HHSC (5x10^5) Size: 1 Cryovial CAT.#: 782-05f Price: $725.00
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Proliferating HHSC, fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 783-25f Price: $725.00
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Proliferating HHSC, fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 783-75f Price: $915.00
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Proliferating HHSC, fetal:  Actively growing, dividing cells in medium Size: 6 Well CAT.#: 783-6Wf Price: $915.00
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Proliferating HHSC, fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 783-96Wf Price: $1,035.00
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Related Products

Product Size CAT.# Price Quantity
Human Hepatic Stellate Cells Basal Medium: Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: 7116-500 Price: $57.00
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Human Hepatic Stellate Cells Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 7117-500 Price: $125.00
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Human Hepatic Stellate Cells Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 7117K-500 Price: $135.00
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Human Hepatic Stellate Cells Growth Medium Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 7117-GS Price: $68.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now