Instructions PBMC (HMNC-PB)
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
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Description

Peripheral Blood Mononuclear Cells (PBMC), also known as Human Mononuclear Cells from Peripheral Blood (HMNC-PB), are widely used in research and clinical applications every day, and provide a useful tool for studying various aspects of pathology and biology in vitro. Cell Applications PBMC are cryopreserved immediately after isolation.
PBMC / HMNC-PB are important tools for studies in:
  • Immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, transplant immunology
  • High-throughput screening
  • Neovascularization to improve limb ischemia and in cell therapy to repair ischemic and infracted myocardium.
  • Human body defense mechanisms critical in the immune system to fight bacterial and viral infections, and function as immune surveillance network to kill tumor cells.
  • The secretion of cytokines to regulate immune response and inflammation
  • Biological effects on tissue and organs.
  • Chemokine effects on adipocytes and muscles
(Click to Enlarge) Peripheral Blood Mononuclear Cells: PBMC, also known as Human Mononuclear Cells from Peripheral Blood (HMNC-PB).

Details

Tissue
Normal healthy human peripheral blood
QC
No bacteria, yeast, fungi, mycoplasma, virus
Cryovial
10,000,000 PBMC / HMNC-PB frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO
Kit
Cryovial Frozen PBMC / HMNC-PB, Culture Medium (615-250)
Cultured
Shipped in Transfer Medium (flasks or plates)
Doublings
N/A - Blood cells don't proliferate in vitro

Products

Product Size CAT.# Price Quantity
Cryopreserved PBMC/HMNC-PB, Adult: 1x10^7 Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 690PBK-100a Price: $334.00
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Cryopreserved PBMC/HMNC-PB, Adult: Frozen PBMC (1x10^7) Size: 1 Cryovial CAT.#: 690PB-100a Price: $278.00
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Cultured PBMC/HMNC-PB, Adult: Fresh Cells in Transfer Medium Size: T-25 Flask CAT.#: 691PB-25a Price: $278.00
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Cultured PBMC/HMNC-PB, Adult: Fresh Cells in Transfer Medium Size: T-75 Flask CAT.#: 691PB-75a Price: $468.00
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Cultured PBMC/HMNC-PB, Adult: Fresh Cells in Transfer Medium Size: 24 Well CAT.#: 691PB-24Wa Price: $468.00
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Cultured PBMC/HMNC-PB, Adult: Fresh Cells in Transfer Medium Size: 96 Well CAT.#: 691PB-96Wa Price: $588.00
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Related Products

Product Size CAT.# Price Quantity
Human Blood Cell Basal Medium: Basal medium (contains no growth supplement).  Add Supplement before use. Size: 250 ml CAT.#: 614-250 Price: $38.00
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Human Blood Cell Culture Medium: Optimized and ready for use. Size: 250 ml CAT.#: 615-250 Price: $66.00
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Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately Size: Yields 250 ml CAT.#: 615K-250 Price: $76.00
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Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium Size: 40 ml CAT.#: 615-CS Price: $40.00
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PBMC Qualified Freezing Medium: Serum-free freezing medium optimized for Peripheral Blood Mononuclear Cells (PBMC) Size: 50 ml CAT.#: 041-50 Price: $74.00
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-CD8: Rabbit CD8 antibody Size: 100 ul CAT.#: CG1649 Price: $289.00
Cryopreserved HPBB, Adult: 2x10^6 CD19+ B Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 6904K-20a Price: $593.00
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Cryopreserved HPBB, Adult: Frozen HPBB, CD19+ (2x10^6) Size: 1 Cryovial CAT.#: 6904-20a Price: $537.00
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Cryopreserved HPBT, Adult: 5x10^6 Cells CD4+ T Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 6902K-50a Price: $431.00
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Cryopreserved HPBT, Adult: Frozen HPBT, CD4+ Size: 1 Cryovial CAT.#: 6902-50a Price: $375.00
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Cultured HPBB, Adult: Fresh CD19+ Cells in Transfer Medium Size: T-25 Flask CAT.#: 6905-25a Price: $537.00
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Cultured HPBB, Adult: Fresh CD19+ Cells in Transfer Medium Size: T-75 Flask CAT.#: 6905-75a Price: $742.00
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Cultured HPBB, Adult: Fresh CD19+ Cells in Transfer Medium Size: 24 Well CAT.#: 6905-24Wa Price: $742.00
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Cultured HPBB, Adult: Fresh CD19+ Cells in Transfer Medium Size: 96 Well CAT.#: 6905-96Wa Price: $871.00
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Cultured HPBT, Adult: Cultured CD4+ Cells in Transfer Medium Size: T-25 Flask CAT.#: 6903-25a Price: $375.00
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Cultured HPBT, Adult: Cultured CD4+ Cells in Transfer Medium Size: T-75 Flask CAT.#: 6903-75a Price: $565.00
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Cultured HPBT, Adult: Cultured CD4+ Cells in Transfer Medium Size: 24 Well CAT.#: 6903-24Wa Price: $565.00
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Cultured HPBT, Adult: Cultured CD4+ Cells in Transfer Medium Size: 96 Well CAT.#: 6903-96Wa Price: $685.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells Size: 50 ml CAT.#: 040-50BC Price: $65.00
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Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 10 ug CAT.#: RP1021AF-10 Price: $213.00
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Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 100 ug CAT.#: RP1021AF-100 Price: $533.00
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Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 1000 ug CAT.#: RP1021AF-1000 Price: $4,499.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 10 ug CAT.#: RP1021-10 Price: $194.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 100 ug CAT.#: RP1021-100 Price: $484.00
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Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand Size: 1000 ug CAT.#: RP1021-1000 Price: $4,090.00
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Mouse CD8 monoclonal Antibody: Mouse CD8 monoclonal antibody Size: 100 ul CAT.#: CP10036 Price: $302.00
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Mouse Cluster of Differentiation 3 Antibody: Mouse Cluster of Differentiation 3 Antibody Size: 100 ul CAT.#: CP10034 Price: $302.00
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Mouse Cluster of Differentiation 4 Antibody: Mouse Cluster of Differentiation 4 Antibody Size: 100 ul CAT.#: CP10035 Price: $302.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2017
Ota, K. and S. Tanaka. 2017. ANIMAL CELL CULTURE MEDIUM AND CULTURE CONTAINER. Patent Application 20170022477 A1.