MSDS Cryopreserved Cells
Instructions PCASMC
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5 Important Cell Culture Rules
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Description
Porcine Coronary Artery Smooth Muscle Cells (PCASMC) from Cell Applications, Inc. were used to show that:
- c-Jun plays a role in smooth muscle proliferation
- Inhibition of c-Jun abrogates smooth muscle cell growth and intimal thickening after arterial injury
- Nanofibrous scaffold material has potential for tissue engineering
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Porcine Coronary Artery Smooth Muscle Cells: PCASMC (L), immunolabeled for smooth muscle-specific alpha-actin using the ABC method (R).
Details
Tissue: | Normal healthy porcine coronary artery |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 PCASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen PCASMC (P350-05), Grwth Medium (P311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Porcine Coronary Artery Smooth Muscle Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: P350K-05 | Price: $949.00 | |
| Cryopreserved Porcine Coronary Artery Smooth Muscle Cells (PCASMC): Frozen PCASMC (5x10^5) | Size: 1 Cryovial | CAT.#: P350-05 | Price: $775.00 | |
| Proliferating Porcine Coronary Artery Smooth Muscle Cells (PCASMC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: P351-25 | Price: $775.00 | |
| Proliferating Porcine Coronary Artery Smooth Muscle Cells (PCASMC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: P351-75 | Price: $965.00 | |
| Proliferating Porcine Coronary Artery Smooth Muscle Cells (PCASMC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: P351-96W | Price: $1,085.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Porcine SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: P310-500 | Price: $74.00 | |
| Porcine SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: P311-500 | Price: $121.00 | |
| Porcine SMC Growth Supplement: Added to Basal Growth Medium to create Growth Medium | Size: 50 ml | CAT.#: P311-GS | Price: $74.00 | |
| Porcine SMC Induction Medium: Provides signals that change cell behavior | Size: 250 ml | CAT.#: P311I-250 | Price: $71.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2012
Khachigian, L. 2012. Vascular therapeutics. Patent US 8242090 B2.
2009
Yixiang, D. 2009. Nanofiber Covered Stent for Vascular Diseases. Thesis, National University of Singapore.
2008
Dong, Y., T. Yong, S. Liao, C.K. Chan, and S. Ramakrishna. 2008. Long-term viability of coronary artery smooth muscle cells on poly (l-lactide-co-ϵ-caprolactone) nanofibrous scaffold indicates its potential for blood vessel tissue engineering. Journal of The Royal Society Interface. 5:1109-1118.
2002
Khachigian, L.M., R.G. Fahmy, G. Zhang, Y.V. Bobryshev, and A. Kaniaros. 2002. c-Jun Regulates Vascular Smooth Muscle Cell Growth and Neointima Formation after Arterial Injury. Journal of Biological Chemistry. 277:22985-22991.