CD34+ Human Hematopoietic Stem Cells from Bone Marrow (HHSC-BM) on day 5 viewed at 20X magnification by phase contrast microscopy (L) and immunofluorescent staining (green) for CD34 (R)
Human CD34+ Hematopoietic Stem Cells from Bone Marrow: HHSC-BM
Human CD34+ Hematopoietic Stem Cells from Bone Marrow (HHSC-BM) are specialized primary stem cells isolated from adult bone marrow tissue and characterized by expression of the CD34 antigen.
Description
Human CD34+ Hematopoietic Stem Cells from Bone Marrow (HHSC-BM) are specialized primary cells isolated from adult tissue, characterized by expression of the CD34 antigen. While frequently referred to as hematopoietic stem cells in shorthand, scientifically, CD34+ preparations represent a heterogeneous population of hematopoietic stem/progenitor cells (HSPC), encompassing both more primitive/less committed stem cells and committed precursors. These primary bm cells (alongside alternative sources such as umbilical cord blood or cord blood and peripheral blood) serve as a critical foundation for studying how hematopoiesis generates diverse mature blood cells and circulating elements in the blood.
Researchers frequently incorporate these primary CD34 cells into investigations involving stem cell self-renewal, progenitor expansion, and lineage differentiation into various blood cell types, including the generation of erythrocyte lineages, megakaryocyte pathways, and myeloid subsets like the granulocyte, as well as lymphoid potential including T cells and B cells. Furthermore, comparative studies often evaluate human hsc populations or broader hematopoietic cells against hematopoietic progenitor cells and progenitor cells derived from a healthy donor or a diagnosed patient, exploring distinct gene regulation profiles and functional capabilities.
Applications and analytical workflows utilizing these primary populations include:
- Colony-Forming Assays and Progenitor Function: Investigators frequently utilize a standard cfu assay (or colony forming unit enumeration) to evaluate the functional capacity and colony-forming potential of hematopoietic progenitors and related populations following experimental manipulation or ex vivo expansion.
- Transplantation and Regenerative Models: Because related HSPC sources (such as mobilized peripheral blood, cord blood, or bone marrow fractions) are foundational for clinical transplantation, researchers use model systems to study engraftment, homing mechanisms, and immune reconstitution. This includes evaluating how specific gene modifications or altered expression profiles affect cell survival, cell division, and behavior within specialized host microenvironments, such as xenotransplantation and engraftment assays in immunodeficient or humanized mice.
- Disease Modeling and Drug Screening: HHSC-BM are frequently used in disease modeling and genetic or therapy studies — including investigating leukemic or lymphoid malignancy–relevant CD34+ populations where alterations drive disease states. Researchers employ advanced techniques like flow cytometry to isolate, characterize, and track specific progenitor fractions and surface marker profiles, supporting a broad range of in vitro hematology and translational research applications.
Human CD34+ Hematopoietic Stem Cells from Bone Marrow (HHSC-BM) are widely used in hematology research and bone marrow-focused studies, particularly in areas related to hematologic malignancies, transplant biology, and bone marrow microenvironment interactions. These primary CD34+ cells are frequently incorporated into research involving stem cell self-renewal, progenitor expansion, and lineage differentiation.
HHSC-BM are also strongly associated with leukemia and lymphoma research, CFU assays, drug screening, and gene therapy workflows, as well as immune reconstitution studies and transplant modeling, supporting a broad range of in vitro hematology and translational research applications.
Details
| Tissue | Normal adult bone marrow | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Character | Positive for CD34 | |
| Bioassay | Maintains in TC ware 7 days | |
| Cryovial | 100, 300, or 500,000 (490BM-01a, -03a, -05a) fresh isolated HHSC cyropreserved in Hematopoietic Cell Freezing Medium (044-50) | |
| Doublings | Can be cultured up to 7 days | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. | |
Resources
FAQs
Need More Help?
Visit our comprehensive FAQ page for detailed answers to common questions.
Need More Help?
Visit our comprehensive FAQ page for detailed answers to common questions.
Primary Cell FAQs
Powered by Bioz