CD34+ Human Hematopoietic Stem Cells from Liver (HHSC-L) stained with CD34/CD45 (Left) and CD34/C38 (Right), and analyzed by flow cytometry. Gates and quartets set by Isotype controls.
Human CD34+ Hematopoietic Stem Cells from Liver: HHSC-L
Human CD34+ Hematopoietic Stem Cells from Liver (HHSC-L) are primary progenitor cells isolated from hepatic tissue.
Description
Human CD34+ Hematopoietic Stem Cells from Liver (HHSC-L) are primary progenitor cells isolated from hepatic tissue, serving as a valuable experimental model for exploring hematopoietic stem and progenitor cell expansion, early hematopoiesis, and blood cell development pathways.
In vivo, the developing and adult hepatic microenvironment supports specialized niches where hematopoietic stem cells interact with resident cell populations, such as hepatic stellate cells and biliary structures within the liver and surrounding parenchymal architecture, before transitioning to primary bone marrow sites. In experimental research, HHSC-L — alongside comparative human cells and mouse models — are frequently incorporated into studies investigating multilineage differentiation, myeloid and lymphoid development, and complex hematopoietic signaling networks. These primary cells support various advanced research applications, including colony-forming unit (CFU) assays, hematopoietic differentiation systems, gene editing workflows (such as CRISPR/Cas9), and viral transduction protocols.
Characterization and tracking of these populations frequently rely on specific cell surface markers, including CD34 (and additional markers as appropriate), evaluated via flow cytometry to verify purity and phenotype. By leveraging these advanced features, researchers can better analyze developmental pathways, evaluate cellular transplantation efficacy, and advance translational models relevant to hematologic disease modeling, regenerative medicine, and broader impacts on human health and medical research.
Human CD34+ Hematopoietic Stem Cells from Liver (HHSC-L) are commonly referenced in research exploring hematopoietic stem and progenitor cell (HSPC) expansion, early hematopoiesis, and blood cell development pathways. These primary CD34+ cells are frequently incorporated into studies involving multilineage differentiation, including myeloid and lymphoid development, as well as hematopoietic signaling networks.
HHSC-L are also associated with colony-forming unit (CFU) assays, hematopoietic differentiation systems, gene editing (CRISPR/Cas9), and viral transduction workflows, supporting research in hematologic disease modeling, immune system biology, and cell therapy development.
Details
| Tissue | Normal fetal liver | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Character | Positive for CD34 | |
| Bioassay | Maintains in TC ware 7 days | |
| Cryovial | 100, 300, or 500,000 (490L-01f, -03f, -05f) fresh isolated HHSC cyropreserved in Hematopoietic Cell Freezing Medium (044-50) | |
| Doublings | Can be cultured up to 7 days | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. | |
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