Human Corneal Keratocytes: HCK Top: Phase-contrast, Bottom Left: HCK immunostained for Vimentin (green), Bottom Right: Fibronectin (green), nuclei are stained with DAPI.
Human Corneal Keratocytes: HCK
Description
Human Corneal Keratocytes (HCK) are primary cells derived from human eye. Corneal Keratocytes are neural-crest derived mesenchymal cells that populate the corneal stroma. The stroma is comprised of a type I and type V collagen fibers arranged as lamellae, makes up majority of the corneal thickness responsible for promoting corneal transparency. Upon corneal injury, keratocytes are stimulated to either undergo apoptosis or transition into repair phenotypes to promote corneal regeneration.
Human Corneal Keratocytes (HCK) cultured in Corneal Keratocyte Growth Medium are able to propagate to 10 population doublings when cultured under the recommended conditions. HCK could be used as an important in vitro cell model to study keratocyte differentiation, corneal injury and related diseases.
Details
| Tissue | Normal healthy human cornea | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 HCK (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen HCK (632-05a), Growth Medium (6111-500), Subculture Rgnt Kit (090K) | |
| Proliferating | Shipped in Tsfr Med, psg 1, flasks or plates | |
| Doublings | At least 15 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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