Human Endothelial Cell Media v2
Human EC Growth Medium V2: All-in-one ready-to-use. Enriched with more growth factors for accelerated cell growth. For angiogenesis studies, use Cat# 211-500.
Type: Growth Medium
Size: 500 ml
Price: $165.00
Description
Human Endothelial Cell Media V2 is a complete, low-serum primary cell culture media designed to support the isolation, expansion, and sustained maintenance of endothelial cells in a standardized cell culture system. Endothelial cells line the inner surface of blood and lymphatic vessels throughout the body and regulate key functions such as barrier integrity, leukocyte trafficking, vascular tone-related signaling, and angiogenic responses. In vitro, primary human endothelial cell cultures are commonly used to model these vascular processes, to study how molecular pathways control endothelial activation and tube formation, and to evaluate how disease-associated factors influence endothelial phenotypes in contexts ranging from inflammation to tumor vascularization.
In a typical endothelial cell growth medium workflow, Human Endothelial Cell Media V2 is provided as a complete medium consisting of a baseline endothelial cell basal medium plus an endothelial cell growth supplement (media supplements packaged for consistent use across experiments). This configuration is intended to create endothelial cell growth conditions with reduced reliance on variable serum lot composition, supporting reproducible cell health and cellular activity while maintaining morphology and functional properties across passage. The formulation is supplied in user-friendly formats with defined packaging information, supports defined application conditions, and is intended to minimize experimental drift by using defined supplemental components (including endothelial-supportive additives) as part of the endothelial cell growth supplement strategy. As with all mammalian cell culture media, appropriate handling and aseptic technique are important to prevent microbial contaminants such as bacteria, fungi, and mycoplasma; products are typically managed with attention to expiry date and storage recommendations to preserve culture properties.
Human Endothelial Cell Media V2 is used most directly for endothelial cell lines and primary human cells, including huvec-derived cultures (HUVEC) and other primary human endothelial cell sources, while also being compatible with endothelial-specific downstream applications that require high-quality cell physiology. In molecular biology workflows, endothelial cultures grown in defined complete medium can be used for gene expression studies, protein analysis, and functional assays that quantify activity of signaling networks following perturbation. Because transfection performance depends on starting cell state, culture conditions that support endothelial cell viability and consistent properties can improve transfection efficiency and the reliability of experimental readouts. For genetic manipulation strategies, endothelial cell cultures may be transduced or transfected using lentiviral vectors or adenoviral systems (for example, endothelial-specific adenoviral overexpression approaches), enabling mechanistic studies of endothelial gene regulation, including experiments relevant to tumor-associated endothelial phenotypes and colorectal cancer models.
Finally, in experimental designs that demand rigorous scientific references and reproducible cell culture medium performance, Human Endothelial Cell Media V2 supports imaging, molecular biology assays, and functional readouts in well-controlled conditions. These properties are valuable when measuring phenotypic endpoints in disease-relevant settings such as tumor endothelial cell culture and when evaluating endothelial responses alongside tumor cell lines. For example, endothelial cells maintained under consistent conditions can be co-cultured with tumor cell lines used in assays relevant to colorectal cancer, and the resulting data quality can depend on how reliably the endothelial cultures maintain activity, properties, and physiological stability throughout the experiment.
Human Endothelial Cell Media v2 — specifically formulated as an Accelerated Growth Medium system — is an optimized, low-serum liquid culture environment designed for the rapid isolation, expansion, and long-term cultivation of primary human endothelial cells.
Unlike standard classical media formulations that lack specialized microenvironmental signals, this v2 generation medium is pre-supplemented with an engineered cocktail of recombinant growth factors, essential lipids, and purified trace elements.
The complete system (Endothelial Cells Growth Medium V2 Kit, 500ml) is supplied as a kit featuring a baseline Endothelial Cell Basal Medium paired with a concentrated Accelerated Growth Medium Supplement. This specific configuration allows investigators to support rapid cell proliferation rates while utilizing a low-serum baseline designed to reduce, serum-related experimental variability.
Media were optimized for the robust growth and the maintenance of phenotypic and functional characteristics of Endothelial Cells in culture. Media were designed with attention to detail that ensures the consistency of cell health, viability, performance, physiology, morphology and function.
Head-to-Head Comparison Peer-Reviewed Article and Video
A recent peer-reviewed article published in Histochemistry and Cell Biology performed a head-to-head comparison of 7 leading cell culture media brands. Based on their results, the authors concluded the following in the publication’s Discussion:
“Fastest colony outgrowth and most colonies occurred in Cell Applications medium.”
See the video below:
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