Human iPSC-Derived Hepatocyte i-HH Media
Human iPSC-Derived Hepatocyte i-HH Media is designed to support the culture, recovery, hepatic differentiation, maturation, and metabolic maintenance of human hepatocyte-like cells generated from an induced pluripotent stem cell (iPSC) or HiPSC source.
Description
Human iPSC-Derived Hepatocyte i-HH Media is designed to support the culture, recovery, hepatic differentiation, maturation, and metabolic maintenance of human hepatocyte-like cells generated from an induced pluripotent stem cell (iPSC) or HiPSC source. These specialized cells are produced through hepatic differentiation of human iPSCs and are intended to model key properties of hepatocytes — the principal parenchymal cells of the liver.
In the body, hepatocytes are organized within the hepatic tissue and form functional units surrounding the liver’s sinusoidal, vascularized network. Together with non-parenchymal cells, including endothelial cells, Kupffer cells, and hepatic stellate cells, they contribute to the multicellular architecture of the liver. Hepatocytes exhibit structural polarization, with distinct sinusoidal and bile-facing surfaces that support the uptake, processing, and secretion of biological molecules.
Hepatocytes perform essential hepatic functions, including albumin production, glycogen storage, urea synthesis, ammonia handling, bile formation, and detoxification of endogenous and exogenous compounds. They also express enzymes and transporters involved in drug metabolism and xenobiotic clearance. Maintaining these functions in vitro is important for evaluating hepatic competence, metabolic activity, and toxic responses in translational research.
The i-HH Recovery, Maturation, and Metabolic Media Kits provide stage-appropriate culture conditions for human iPSC-derived hepatocyte-like cells. The media can help support cell recovery following handling or plating, promote the development and maintenance of a more specialized hepatic phenotype, and sustain metabolic functions required for in vitro assays. These capabilities may be valuable when working with non-malignant, patient-specific cells, including cells generated from disease-relevant or genetically diverse iPSC lines.
Human iPSC-derived hepatocyte-like cells cultured with i-HH media may be used in drug metabolism studies, compound screening, biomarker analysis, regenerative medicine research, and investigations of drug-induced liver injury and hepatotoxicity. They can also be incorporated into organoid, co-culture, and other biomimetic model systems designed to reproduce aspects of human liver biology. When combined with endothelial or other non-parenchymal cells, these models may provide a more physiologically relevant multicellular context than hepatocyte monocultures or immortalized cell lines.
By supporting hepatic phenotype and function in vitro, Human iPSC-Derived Hepatocyte i-HH Media can assist researchers — including pharmacologists and toxicologists — in developing predictive assays for drug metabolism, toxic or xenobiotic responses, and liver safety. The resulting models may complement primary human hepatocytes and immortalized hepatic cell systems while offering opportunities for patient-specific, regenerative, and translational applications.