Porcine Pulmonary Artery Smooth Muscle Cells: PPASMC
Porcine Pulmonary Artery Smooth Muscle Cells: PPASMC
Porcine Pulmonary Artery Smooth Muscle Cells (PPASMC) are specialized vascular smooth muscle cells derived from the pulmonary arteries of swine.
Description
Porcine Pulmonary Artery Smooth Muscle Cells (PPASMC) provide a useful in vitro model to study cellular and molecular mechanisms of pulmonary hypertension, a condition often associated with the aberrant proliferation of pulmonary artery smooth muscle cells. As specialized vascular smooth muscle cells derived from the pulmonary arteries of swine, they serve as an important experimental system in molecular biology and cardiovascular research.
In vivo, these cells reside within the medial layer of the pulmonary vasculature, where they contribute to vascular tone, structural integrity, and the regulation of blood flow through the pulmonary circulation. In experimental models, PPASMC can be used to investigate pathological processes such as pulmonary vascular remodeling, hypoxia-induced cell proliferation, and the molecular signaling cascades underlying pulmonary arterial hypertension. Researchers can utilize these cells to explore:
- Alterations in protein expression, mRNA expression, and transcription factor activation during smooth muscle cell growth and pathological remodeling.
- The effects of pharmacological agents, such as rapamycin, and varying concentrations of growth factors on cell proliferation across different oxygen conditions like normoxia and hypoxia.
- Comparative responses and biological processes involving related cell and tissue populations, including endothelial cells, mesenchymal stem cells, fibroblasts, epithelial cells, and stellate cells.
Investigators typically characterize these cultures by confirming positive expression for smooth muscle cell-specific alpha-actin. Typically supplied as frozen samples in a vial stored at cryogenic temperatures, these primary cells are maintained under controlled cell culture conditions at 37°C across successive passages to support rigorous investigations into vascular disease and right heart failure mechanisms.
Porcine Pulmonary Artery Smooth Muscle Cells (PPASMC) provide a useful in vitro model to study cellular and molecular mechanisms of pulmonary hypertension characterized by hyperproliferation of pulmonary artery smooth muscle cells.
Characterization: positive for smooth muscle cell specific alpha-actin expression
Details
| Tissue: | Normal healthy porcine pulmonary artery | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Character | Smooth muscle specific α-actin positive | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 PPASMC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen PPASMC (P352-05), Growth Medium (P311-500), Subcltr Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) | |
| Doubllings | At least 16 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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