Cryopreserved Rat Dorsal Root Ganglion Neurons (RDRGN) revived and cultured for 6 days (top), stained (bottom) on day 14 for β III-Tubulin (green), S100β (red), and DAPI (blue).
Rat Dorsal Root Ganglion Neurons (RDRGN) lie along the vertebral column by the spine and are nerve cell body clusters containing afferent neurons that relay sensory information from the peripheral body to the CNS. They are used to study ion channel properties, modulation, neuropathy, peripheral nerve injury, regeneration, myelination, synaptogenesis & more.
Rat Ganglion Neuron Medium vs Competition
Figure: Superior Growth of Neurons Cultured in Cell Applications Medium versus Competitor.
Notably, Rat Dorsal Root Ganglion Neurons exhibit superior viability and neurite extensions when cultured in optimized media from Cell Applications, compared to a leading competitor medium. In the above Figure, RDRGN (Cat # R8820N-05) were cultured at a density of at 1×10^5 cells/cm2 in Rat Ganglion Neuron Culture Medium from Cell Applications, Inc. (Top) or competitor Neuron Medium + 100ng/ml rNGF + mitotic inhibitors (Bottom). Microscopic images were captured at days 1, 2 & 7, clearly showing optimal growth in the CAI medium.
High quality Rat Dorsal Root Ganglion Neurons (RDRGN) are derived from spinal cords of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions with specialty culture medium, cell death occurs during the first few days after plating. The remaining pure RDRGN arborize and form complex neurite network in one week. RDRGN Stain positive for β III-Tubulin.
Details
Tissue
Normal healthy rat spinal cord
QC
No bacteria, yeast, fungi, mycoplasma
Character
Positive for β-III Tubulin
Bioassay
Cells plate on Neuron Coating Solution II-coated surface, Arborize to form neurite network in Culture Medium
Cryovial
1,000,000 or 500,000 RDRGN in Ser-Fr Frzng Med (042-50)
Kit
Cryovial RDRGN, Ctng Soln II, Pltng Med, Cultr Med Kit
Doublings
N/A – Neurons don’t proliferate in vitro
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
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