Instructions RLF
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Airway Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Lung Fibroblasts (RLF) from Cell Applications, Inc. have been used to demonstrate that exaggerated (compared to adults) NF-κB activation in neonates in response to hyperoxia is mediated by phosphorylation of IκBα on Tyr42. They were also used to elucidate melatonin effects and signaling pathway and served as a control in studies investigating epithelial-mesenchimal transition as the source of myofibroblasts responsible for idiopathic pulmonary fibrosis.
(Click to Enlarge) Rat Lung Fibroblasts: RLF. Isolated directly from rodent pulmonary tissue.
Details
Tissue | Normal healthy Sprague Dawley rat lung |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Positive for fibroblast surface protein |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 RLF (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen RLF (R506-05a/-05n), Grwth Med (R116-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 10 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Lung Fibroblast Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R506K-05a | Price: $571.00 | |
| Cryopreserved Rat Lung Fibroblasts (RLF), adult: Frozen RLF (5x10^5) | Size: 1 Cryovial | CAT.#: R506-05a | Price: $404.00 | |
| Proliferating Rat Lung Fibroblasts (RLF), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R507-25a | Price: $404.00 | |
| Proliferating Rat Lung Fibroblasts (RLF), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R507-75a | Price: $594.00 | |
| Proliferating Rat Lung Fibroblasts (RLF), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R507-24Wa | Price: $594.00 | |
| Proliferating Rat Lung Fibroblasts (RLF), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R507-96Wa | Price: $714.00 | |
| Cryopreserved Rat Lung Fibroblast Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R506K-05n | Price: $571.00 | |
| Cryopreserved Rat Lung Fibroblasts (RLF), neonatal: Frozen RLF (5x10^5) | Size: 1 Cryovial | CAT.#: R506-05n | Price: $404.00 | |
| Proliferating Rat Lung Fibroblasts (RLF), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R507-25n | Price: $404.00 | |
| Proliferating Rat Lung Fibroblasts (RLF), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R507-75n | Price: $594.00 | |
| Proliferating Rat Lung Fibroblasts (RLF), neonatal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R507-24Wn | Price: $594.00 | |
| Proliferating Rat Lung Fibroblasts (RLF), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R507-96Wn | Price: $714.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Fibroblast Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R115-500 | Price: $74.00 | |
| Rat Fibroblast Growth Medium: All-in-one ready-to-use media | Size: 500 ml | CAT.#: R116-500 | Price: $114.00 | |
| Rat Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: R116K-500 | Price: $124.00 | |
| Rat Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: R116-GS | Price: $66.00 | |
| Rat Fibroblast Basal Medium wo Phenol Red: Basal medium (contains no growth supplement). Add GS before use. Without Phenol Red. | Size: 500 ml | CAT.#: R115PR-500 | Price: $86.00 | |
| Rat Fibroblast Growth Medium wo FBS: Growth Medium without FBS | Size: 500 ml | CAT.#: R116F-500 | Price: $131.00 | |
| Rat Fibroblast Growth Supplement wo FBS: Growth Supplement without FBS | Size: 50 ml | CAT.#: R116F-GS | Price: $75.00 | |
| Rat Fibroblast Growth Medium wo Phenol Red: All-in-one ready-to-use media, without Phenol Red | Size: 500 ml | CAT.#: R116PR-500 | Price: $131.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF103K | Price: $465.00 | |
| Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF103KS | Price: $62.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 10 ug | CAT.#: RP3027-10 | Price: $194.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 100 ug | CAT.#: RP3027-100 | Price: $484.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 1000 ug | CAT.#: RP3027-1000 | Price: $4,090.00 | |
| Rat Lung Fibroblast RNA (RLF RNA), Adult : Total RNA prepared from Rat Lung Fibroblasts | Size: 10 ug | CAT.#: R506-R10a | Price: $398.00 | |
| Rat Lung Fibroblast RNA (RLF RNA), Adult : Total RNA prepared from Rat Lung Fibroblasts | Size: 25 ug | CAT.#: R506-R25a | Price: $796.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2017
Kim, H., K. Jang, J. Shin, D. Kang, S. Kim, I. Kob, Y. Hong, S. Jang, M. Kim, B. Kim, H. Jeong, N. Jeong, P. Kim and K. Suh. 2017. Artificial Slanted Nanocilia Array as a Mechanotransducer for Controlling Cell Polarity. ACS Nano, DOI: 10.1021/acsnano.6b07134.
2012
Soussia, I., F. Mies, R. Naeije, and V. Shlyonsky. 2012. Melatonin down-regulates volume-sensitive chloride channels in fibroblasts. Pflugers Arch - Eur J Physiol. 464:273-285.
2009
Wright, C.J., T. Zhuang, P. La, G. Yang, and P.A. Dennery. 2009. Hyperoxia-induced NF-κB activation occurs via a maturationally sensitive atypical pathway. American Journal of Physiology. 296:L296-L306.
2007
Jain, R., P.W. Shaul, Z. Borok, and B.C. Willis. 2007. Endothelin-1 induces alveolar epithelial–mesenchymal transition through endothelin type A receptor–mediated production of TGF-β1. American J. Resp. Cell & Mol.Biol. 37:38.
2005
Willis, B.C., J.M. Liebler, K. Luby-Phelps, A.G. Nicholson, E.D. Crandall, R.M. du Bois, and Z. Borok. 2005. Induction of Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells by Transforming Growth Factor-β1: Potential Role in Idiopathic Pulmonary Fibrosis. The American journal of pathology. 166:1321-1332.