Porcine Pulmonary Artery Endothelial Cells: PPAEC
Porcine Pulmonary Artery Endothelial Cells: PPAEC
Description
Porcine Pulmonary Artery Endothelial Cells (PPAEC) from Cell Applications, Inc. have been used to:
- Determine that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP
- This event led to downregulation of Rac1, and induction of stress fibers & focal adhesions via a RhoA/ROCK dependent mechanism
- The resulting decreased cell motility, and increase in endothelial permeability, linked vascular repair and function with NO production
- PPAEC have also been used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair
Details
| Tissue | Normal healthy porcine pulmonary artery | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Character | DiI-Ac-LDL uptake: Positive | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 PPAEC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen PPAEC (P302-05), Growth Medium (P211-500), Subculture Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) | |
| Doublings | At least 16 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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