5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Lying along the vertebral column by the spine, Rat Dorsal Root Ganglion Neurons (RDRGN) are nerve cell body clusters containing afferent neurons that relay sensory information from the peripheral body to the CNS. They are used to study ion channel properties, modulation, neuropathy, peripheral nerve injury, regeneration, myelination, synaptogenesis & more.
Figure: Superior Growth of Neurons Cultured in Cell Applications Medium versus Competitor.
Notably, Rat Dorsal Root Ganglion Neurons exhibit superior viability and neurite extensions when cultured in optimized media from Cell Applications, compared to a leading competitor medium. In the above Figure, RDRGN (Cat # R8820N-05) were cultured at a density of at 1x10^5 cells/cm2 in Rat Ganglion Neuron Culture Medium from Cell Applications, Inc. (Top) or competitor Neuron Medium + 100ng/ml rNGF + mitotic inhibitors (Bottom). Microscopic images were captured at days 1, 2 & 7, clearly showing optimal growth in the CAI medium.
High quality Rat Dorsal Root Ganglion Neurons (RDRGN) are derived from spinal cords of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions with specialty culture medium, cell death occurs during the first few days after plating. The remaining pure RDRGN arborize and form complex neurite network in one week. RDRGN Stain positive for β III-Tubulin.
|Tissue||Normal healthy rat spinal cord|
|QC||No bacteria, yeast, fungi, mycoplasma|
|Character||Positive for β-III Tubulin|
|Bioassay||Cells plate on Neuron Coating Solution II-coated surface, Arborize to form neurite network in Culture Medium|
|Cryovial||500,000 RDRGN in Ser-Fr Frzng Med (042-50)|
|Fresh Culture||Contact CAI if interested in freshly isolated RDRGDN (suspension or plated)|
|Kit||Cryovial RDRGN, Ctng Soln II (029-05), Pltng Med (R823P-10), Cultr Med Kit (R823K-50)|
|Doublings||N/A - Neurons don't proliferate in vitro|
|Applications||Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.|
|Cryopreserved RDRGN: Frozen RDRGN (1x10^6)||Size: 1 Cryovial||CAT.#: R8820N-10||Price: $940.00|
|Cryopreserved RDRGN: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics)||Size: 1 Kit||CAT.#: R8820NK-10||Price: $1,116.00|
|Freshly isolated RDRGN: Contact CAI for details||Size: 1 Tube||CAT.#: R8821NF-05||Price: $0.00|
|Neuron Coating Solution II: Poly-D-Lysine coating for neurons, especially RDGRN and HBN.||Size: 5 ml||CAT.#: 029-05||Price: $27.00|
|Rat Ganglion Neuron Culture Medium Kit: Basal Medium and 5 Supplements bottled separately||Size: Yields 50 ml||CAT.#: R823K-50||Price: $75.00|
|Rat Ganglion Neuron Culture Medium Kit: Basal Medium and 5 Supplements bottled separately||Size: Yields 100 ml||CAT.#: R823K-100||Price: $128.00|
|Rat Ganglion Neuron Plating Medium: Use for initial plating of ganglion neurons.||Size: 10 ml||CAT.#: R823P-10||Price: $20.00|
|Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium||Size: 3 ml||CAT.#: R817-GS||Price: $57.00|
Extended Family Products
|Cyto-X Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $139.00|
|Cyto-X Cell Counting Reagent Sample: 100 tests||Size: Sample||CAT.#: 028-S||Price: $36.00|
|Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns||Size: 1 Kit||CAT.#: TF886K||Price: $404.00|
|Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns||Size: 1 Sample Kit||CAT.#: TF886KS||Price: $54.00|