Instructions RDRGN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Dorsal Root Ganglion Neurons (RDRGN) lie along the vertebral column by the spine and are nerve cell body clusters containing afferent neurons that relay sensory information from the peripheral body to the CNS. They are used to study ion channel properties, modulation, neuropathy, peripheral nerve injury, regeneration, myelination, synaptogenesis & more.
Figure: Superior Growth of Neurons Cultured in Cell Applications Medium versus Competitor.
Notably, Rat Dorsal Root Ganglion Neurons exhibit superior viability and neurite extensions when cultured in optimized media from Cell Applications, compared to a leading competitor medium. In the above Figure, RDRGN (Cat # R8820N-05) were cultured at a density of at 1x10^5 cells/cm2 in Rat Ganglion Neuron Culture Medium from Cell Applications, Inc. (Top) or competitor Neuron Medium + 100ng/ml rNGF + mitotic inhibitors (Bottom). Microscopic images were captured at days 1, 2 & 7, clearly showing optimal growth in the CAI medium.
High quality Rat Dorsal Root Ganglion Neurons (RDRGN) are derived from spinal cords of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions with specialty culture medium, cell death occurs during the first few days after plating. The remaining pure RDRGN arborize and form complex neurite network in one week. RDRGN Stain positive for β III-Tubulin.
(Click to Enlarge) Cryopreserved Rat Dorsal Root Ganglion Neurons (RDRGN) revived and cultured for 6 days (top), stained (bottom) on day 14 for β III-Tubulin (green), S100β (red), and DAPI (blue).
Details
| Tissue | Normal healthy rat spinal cord |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for β-III Tubulin |
| Bioassay | Cells plate on Neuron Coating Solution II-coated surface, Arborize to form neurite network in Culture Medium |
| Cryovial | 1,000,000 or 500,000 RDRGN in Ser-Fr Frzng Med (042-50) |
| Kit | Cryovial RDRGN, Ctng Soln II, Pltng Med, Cultr Med Kit |
| Doublings | N/A - Neurons don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Dorsal Root Ganglion Neurons Total Kit, 1E6: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) | Size: 1 Kit | CAT.#: R8820NK-10 | Price: $1,321.00 | |
| Cryopreserved Rat Dorsal Root Ganglion Neurons (RDRGN): Frozen RDRGN (1x10^6) | Size: 1 Cryovial | CAT.#: R8820N-10 | Price: $1,100.00 | |
| Cryopreserved Rat Dorsal Root Ganglion Neurons Total Kit, 5E5: 5x10^5 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) | Size: 1 Kit | CAT.#: R8820NK-05 | Price: $828.00 | |
| Cryopreserved Rat Dorsal Root Ganglion Neurons (RDRGN): Frozen RDRGN (5x10^5) | Size: 1 Cryovial | CAT.#: R8820N-05 | Price: $698.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Dorsal Root Ganglion Neuron Culture Medium Kit: Basal Medium and 5 Supplements bottled separately | Size: Yields 100 ml | CAT.#: R823K-100 | Price: $147.00 | |
| Rat Dorsal Root Ganglion Neuron Culture Medium Kit: Basal Medium and 5 Supplements bottled separately | Size: Yields 50 ml | CAT.#: R823K-50 | Price: $86.00 | |
| Rat Ganglion Neuron Plating Medium: Use for initial plating of ganglion neurons. | Size: 10 ml | CAT.#: R823P-10 | Price: $23.00 | |
| Neuron Coating Solution II: Poly-D-Lysine and Laminin coating solution for neurons, especially RDGRN and HBN. | Size: 5 ml | CAT.#: 029-05 | Price: $31.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $465.00 | |
| Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $62.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 |
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