Instructions HUAEC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
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Description
Human Umbilical Artery Endothelial Cells (HUAEC) provided by Cell Applications, Inc. have been used to investigate the influence of soluble factors secreted by tumor cells on the expression of cell surface antigens by endothelial cells (Moehler, 2008). It was determined that tumor-derived factors activated transcription of FUT1 which lead to enhanced expression of CD174, a molecule involved in cell-cell adhesion and migration during tumor angiogenesis.
(Click to Enlarge) Human Umbilical Artery Endothelial Cells, HUAEC (L). HUAEC immunolabeled with anti-vWF antibodies (green); nuclei are stained with PI (red) (R).
Details
Tissue | Normal healthy human umbilical arteries |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Factor VIII-related Ag, DiI-Ac-LDL |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HUAEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HUAEC, Growth Medium (212-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 15 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Umbilical Artery Endothelial Cells Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 202K-05n | Price: $619.00 | |
| Cryopreserved Umbilical Artery Endothelial Cells (HUAEC), neonatal: Frozen HUAEC (5x10^5) | Size: 1 Cryovial | CAT.#: 202-05n | Price: $430.00 | |
| Proliferating Umbilical Artery Endothelial Cells (HUAEC), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 203-25n | Price: $430.00 | |
| Proliferating Umbilical Artery Endothelial Cells (HUAEC), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 203-75n | Price: $620.00 | |
| Proliferating Umbilical Artery Endothelial Cells (HUAEC), neonatal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 203-24Wn | Price: $620.00 | |
| Proliferating Umbilical Artery Endothelial Cells (HUAEC), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 203-96Wn | Price: $740.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Meso Endo Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 212-500 | Price: $136.00 | |
| Human Meso Endo Growth Medium wo Antibiotics: Growth medium without antibiotics | Size: 500 ml | CAT.#: 212A-500 | Price: $143.00 | |
| Human Meso Endo Growth Medium wo Phenol Red: Growth medium without phenol red | Size: 500 ml | CAT.#: 212PR-500 | Price: $136.00 | |
| Human Meso Endo Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: 212K-500 | Price: $147.00 | |
| Human Meso Endo Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red | Size: yields 500 ml | CAT.#: 212KPR-500 | Price: $150.00 | |
| Human Meso Endo Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 212-GS | Price: $81.00 | |
| Human Meso Endo Growth Medium wo FBS: Growth Medium without FBS | Size: 500 ml | CAT.#: 212F-500 | Price: $156.00 | |
| Human Meso Endo Growth Supplement wo FBS: Growth Supplement without FBS | Size: 5 ml | CAT.#: 212F-GS | Price: $88.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | Size: 100 ul | CAT.#: CG1238 | Price: $275.00 | |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | Size: 100 ul | CAT.#: CB3778 | Price: $302.00 | |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | Size: 100 ul | CAT.#: CB3839 | Price: $333.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | Size: 96 Wells | CAT.#: CL0501 | Price: $587.00 | |
| Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0370 | Price: $484.00 | |
| Umbilical Artery Endothelial Cell RNA (HUAEC RNA), Neonatal: Total RNA prepared from Human Umbilical Artery Endothelial Cells, neonatal | Size: 10 ug | CAT.#: 202-R10n | Price: $398.00 | |
| Umbilical Artery Endothelial Cell RNA (HUAEC RNA), Neonatal: Total RNA prepared from Human Umbilical Artery Endothelial Cells, neonatal | Size: 25 ug | CAT.#: 202-R25n | Price: $796.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 25 ug | CAT.#: RP1127-25 | Price: $194.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 100 ug | CAT.#: RP1127-100 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 1000 ug | CAT.#: RP1127-1000 | Price: $3,175.00 | |
| Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0505 | Price: $517.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 10 ug | CAT.#: RP1116-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 100 ug | CAT.#: RP1116-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 1000 ug | CAT.#: RP1116-1000 | Price: $4,090.00 | |
| Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit | Size: 96 Wells | CAT.#: CL0588 | Price: $581.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 10 ug | CAT.#: RP1116AF-10 | Price: $213.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 100 ug | CAT.#: RP1116AF-100 | Price: $533.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 1000 ug | CAT.#: RP1116AF-1000 | Price: $4,499.00 |
Resources/Documents
Citations
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2015
Bowers, S., Meng, C., Davis, M., and Davis, G. 2015. Investigating Human Vascular Tube Morphogenesis and Maturation Using Endothelial Cell-Pericyte Co-cultures and a Doxycycline-Inducible Genetic System in 3D Extracellular Matrices. Methods in Molecular Biology, 1189:171-189.
2008
Moehler, T.M., S. Sauer, M. Witzel, M. Andrulis, J.J. Garcia‐Vallejo, R. Grobholz, M. Willhauck‐Fleckenstein, A. Greiner, H. Goldschmidt, and R. Schwartz‐Albiez. 2008. Involvement of α 1‐2‐fucosyltransferase I (FUT1) and surface‐expressed lewisy (CD174) in first endothelial cell–cell contacts during angiogenesis. J. Cell. Phys. 215:27-36.