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Human Endothelial Cell Media

Human EC Growth Medium: All-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies. For accelerated growth, use Cat# 213-500.

Type: Growth Medium
Size: 500 ml

CAT. #: 211-500

Price: $139.00

Quantity

Description

Human
Human

Media were optimized for the robust growth and the maintenance of phenotypic and functional characteristics of Endothelial Cells in culture. Media were designed with attention to detail that ensures the consistency of cell health, viability, performance, physiology, morphology and function.

Use with:

EGM v2 (Cat# 213-500) supports continuous, robust growth 

HUVEC were seeded to T25 flasks, passaged repeatedly, and counted for cell number.
1803 HUVEC EGM2 growth curve v2
1803 HUVEC EGM2 growth curve v2

Sustained expression of endothelial cell markers

Growth of primary Human Aortic Endothelialial Cells (HAOEC)  in EGM (Cat# 211-500) maintains CD31 expression.
1803 HAOEC CD31 staining
1803 HAOEC CD31 staining

Following treatment of TNF-alpha, Human Coronary Artery Endothelial cells (HCAEC) upregulate VCAM-1 (right). Untreated (left)
1803 HCAEC+TNFa-VCAM 1 staining v2
1803 HCAEC+TNFa-VCAM 1 staining v2

2D angiogenesis assay using HUVEC co-cultured with HDF 

For 10 days, cells were stimulated with VEGF or VEGF in the presence of the angiogenesis inhibitor suramin.

1803 HUVEC+HDF 3D angiogenesis CD31 staining 

Transfect primary endothelial cells with Cytofect Transfection Kits 

Convenient, non-toxic transfection of HAOEC with GFP plasmid. 1803 HAOEC transfection

Client Testimonial

According to Sara Lynn Farwell, Ph.D. Candidate, Cell & Molecular Bio. at Lehigh University:  “CAI’s Endothelial Cell Growth Medium is reliable for our experiments, as well as general culturing.  There must be magic in their media, because when we had problems with other formulations, the CAI product fixed the issues!”

Note that endothelial Cell Defined Medium is designed for maintaining the monolayer of endothelial cells at 100% confluent culture. It does not contain the growth factors VEGF, FGF or PDGF, nor does it contain serum, but is instead supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors.

Details

CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions.  The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
MSDS 211-500

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MSDS 211K-500

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MSDS 210-500

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MSDS 211-GS

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Citations

Extended Family Products

PRODUCTSIZECAT.#PRICEQUANTITY
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution1 Kit, 100 ml090K$69.00
HUVEC Conditioned Medium: HUVEC Conditioned Medium5 ml211CM-05$62.00
Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monolayers in confluent cultures. Not for cell growth.500 ml113-500$143.00
Human EC Serum-Free Defined Medium wo Phenol Red: 500 ml113PR-500$150.00
Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns1 KitTF101K$546.00
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.50 ml040-50$54.00
Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns1 Sample KitTF101KS$68.00
HUVEC Conditioned Medium: HUVEC Conditioned Medium
Size: 5 mlCat.#: 211CM-05Price: $62.00
Human EC Serum-Free Defined Medium: Defined medium without serum designed to maintain endothelial cell monolayers in confluent cultures. Not for cell growth.
Size: 500 mlCat.#: 113-500Price: $143.00
Size: 500 mlCat.#: 113PR-500Price: $150.00
Size: 1 KitCat.#: TF101KPrice: $546.00
Size: 1 Sample KitCat.#: TF101KSPrice: $68.00