Freshly isolated Rat Hippocampal Neurons (RHiN) cultured for 5 days (A). Freshly isolated RHiN (B), and revived cryopreserved RHiN (C) stained on day 8 for β III-Tubulin (green), GFAP (red), and DAPI (blue).
Rat Hippocampal Neurons: RHiN
Description
Rat Hippocampal Neurons (RHiN) are derived from the hippocampi of normal embryonic rats by standardized methods. When cultured under the recommended conditions, RHiN arborize and form complex neurite network in one week. RHiN Stain positive for β III-Tubulin.
The hippocampus, a major brain component located under the cerebral cortex, consists of two hippocampi, one in each side of the brain. Belonging to the limbic system, the hippocampus functions in spacial memory and navigation, and the consolidation of information from short- to long-term memory. Hippocampal neurons provide a model in vitro system for studying neurophysiology, neural plasticity and memory storage. Damage, disease or oxygen starvation in this region is associated with Alzheimer’s disease, encephalitis, epilepsy and amnesia. Examining hippocampal neurons in those disease conditions will allow a better understanding of brain physiology, disease mechanisms, pathology, and foster advancements in drug development and ultimate clinical applications.
Details
| Tissue | Normal healthy rat hippocampus | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Character | Positive for β-III Tubulin | |
| Bioassay | Plate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med | |
| Cryovial | 1M RHiN cryopreserved after isolation in Serum-Free Freezing Med (042-50) | |
| Cryo Kit | RHiN (R886N-10), Cltr Med (R817-100) Ctng Soln I (027-05), Pltng Med (R886P-10) | |
| Doublings | N/A – Neurons don’t proliferate in vitro | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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