Instructions RPASMC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Airway Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Pulmonary Artery Smooth Muscle Cells (RPASMC) provide a useful in vitro model to study cellular and molecular mechanisms of pulmonary hypertension characterized by hyperproliferation of pulmonary artery smooth muscle cells.
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Rat Pulmonary Artery Smooth Muscle Cells: RPASMC (L), immunolabeled for smooth muscle-specific alpha-actin by ABC method (R).
Details
Tissue | Normal healthy rat pulmonary artery |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 RPASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen RPASMC (R352-05a), Growth Medium (R311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 12 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Pulmonary Artery Smooth Muscle Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R352K-05a | Price: $896.00 | |
| Cryopreserved Rat Pulmonary Artery Smooth Muscle Cells (RPASMC), adult: Frozen RPASMC (5x10^5) | Size: 1 Cryovial | CAT.#: R352-05a | Price: $716.00 | |
| Proliferating Rat Pulmonary Artery Smooth Muscle Cells (RPASMC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R353-25a | Price: $716.00 | |
| Proliferating Rat Pulmonary Artery Smooth Muscle Cells (RPASMC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R353-75a | Price: $906.00 | |
| Proliferating Rat Pulmonary Artery Smooth Muscle Cells (RPASMC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R353-24Wa | Price: $906.00 | |
| Proliferating Rat Pulmonary Artery Smooth Muscle Cells (RPASMC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R353-96Wa | Price: $1,026.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R310-500 | Price: $78.00 | |
| Rat SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: R311-500 | Price: $127.00 | |
| Rat SMC Growth Medium wo FBS: Growth medium without FBS | Size: 500 ml | CAT.#: R311F-500 | Price: $136.00 | |
| Rat SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: R311K-500 | Price: $136.00 | |
| Rat Smooth Muscle Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: yields 500 ml | CAT.#: R311K-500* | Price: $118.39 | |
| Rat SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: R311-GS | Price: $77.00 | |
| Rat SMC Induction Medium: Provides signals that change cell behavior | Size: 250 ml | CAT.#: R311I-250 | Price: $78.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Mouse Interleukin-6 (IL-6): Mouse Interleukin-6 | Size: 10 ug | CAT.#: RP2002-10 | Price: $194.00 | |
| Mouse Interleukin-6 (IL-6): Mouse Interleukin-6 | Size: 100 ug | CAT.#: RP2002-100 | Price: $624.00 | |
| Mouse Interleukin-6 (IL-6): Mouse Interleukin-6 | Size: 1000 ug | CAT.#: RP2002-1000 | Price: $5,327.00 | |
| Rat IL-6 ELISA Kit: Rat Interleukin-6 ELISA Kit | Size: 96 Wells | CAT.#: CL0412 | Price: $484.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Publications
2016
Dalvi, P., L. Spikes, J. Allen, V. Gupta, H. Sharma, M. Gillcrist, J. do Oca, A. O’Brien-LKadner and N. Dhillon. 2016. Effect of Cocaine on Pulmonary Vascular Remodeling and Hemodynamics in Human Immunodeficiency Virus–Transgenic Rats. Am J Respiratory Cell & Molec Biol, 55:201-212.