Human Tracheal Epithelial Cells: HTEpC-AS
Human Tracheal Epithelial Cells: Asthma: HTEpC-AS
Human Tracheal Epithelial Cells (HTEpC-AS) are a primary cell type isolated from the surface epithelial cells of the human trachea from donors with asthma.
Description
Human Tracheal Epithelial Cells from donors with asthma (HTEpC-AS) provide a useful model system to study key aspects of epithelial function and disease, particularly when investigating pathways relevant to studying epithelial responses observed in airway viral infections, tissue repair mechanisms, signaling changes, and associated cellular stressors. Sourced specifically from patients with asthma, these primary cells serve as an important experimental model for examining disease-specific respiratory tract physiology.
In vivo, the human trachea and broader respiratory epithelium form the primary lining of the conducting airways, where surface epithelial cells, ciliated cells, and secretory elements work together to maintain mucociliary clearance and defend against inhaled pathogens and environmental irritants. Altered barrier properties and epithelial-derived inflammatory signaling are key features of chronic respiratory conditions. Asthma is a chronic disease that inflames and narrows air passageways in the lungs, causing airflow obstruction, shortness of breath, and potentially life-threatening flare-ups. Patients managing asthma must monitor symptoms, identify asthma triggers, recognize early warning signs of an asthma attack, and follow a personalized asthma action plan focused on asthma control and treatment.
In experimental research, in vitro HTEpC-AS culture systems allow scientists to examine specific epithelial contributions to airway inflammation and barrier dysfunction when cultured under appropriate conditions. For example, these cells can be used to investigate pathways associated with secretory cell phenotypes and cytokine signaling relevant to chronic airway diseases. Researchers also utilize these cell models to study mucosal responses to therapeutic pathway perturbations and related anti-inflammatory interventions.
Investigators typically characterize these cultures by confirming a morphology consistent with epithelial origin and positive staining for epithelial cell markers such as cytokeratin 18. Maintaining experimental integrity requires rigorous screening against mycoplasma to ensure reliable results when evaluating epithelial responses derived from patients with airway disorders.
Human Tracheal Epithelial Cells (HTEpC) provide a useful model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, pulmonary diseases and smoking. Note that Cell Applications, Inc. offers HTEpC obtained from healthy donors, as well as from asthma patients.
HTEpC from Cell Applications, Inc. have been used in a study investigating the role of goblet cell hyperplasia, possibly mediated by the expression of SPDEF, to the pathogenesis of chronic pulmonary diseases.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
Cell Applications offers a wide panel of cells for use in asthma research and airway drug development, such as Bronchial & Tracheal Epithelial Cells, Pulmonary & Lung Microvascular Endothelial cells and others. Multiple donor profiles and lots are available. Asthma (AS) is a chronic disease that inflames and narrows air passageways in the lungs. This airflow obstruction, which can flare up at any time, causes shortness of breath and can be life-threating in severe cases. Asthma often starts during childhood, and while there’s no cure, scientists and pharmaceutical companies have made strides in understanding, treating and managing the disease.
Details
| Tissue | Surface epithelium of human trachea from donor with asthma | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Bioassay | Attach, spread, proliferate in Growth Medium | |
| Cryovial | 500,000 HTEpC-AS (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen HTEpC-AS (504AS-05a), Growth Medium (511-500), Subcltr Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) | |
| Doublings | At least 16 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Resources
FAQs
Need More Help?
Visit our comprehensive FAQ page for detailed answers to common questions.
Need More Help?
Visit our comprehensive FAQ page for detailed answers to common questions.
Primary Cell FAQs