Re-Differentiated Human Chondrocytes (HC-RD), encapsulated in an alginate bead suspension.
Human Chondrocytes: Re-Differentiated: HC-RD
Description
Human Chondrocytes Re-Differentiated (HC-RD) have been encapsulated in an alginate bead suspension. HC-RD exhibit spherical morphology and have regained their ability to produce the phenotypic markers Aggrecan and Type II Collagen. RD-HC can be used in tissue engineering experiments aimed to repair cartilage defects and to study chondrocyte differentiation and the effects of Growth factors & Cytokines on the suppression and maintenance of differentiated cartilage phenotype.
Instructions to release HC-RD from Alginate Beads
- Remove Chondrocyte Differentiation Medium
- Wash the beads 2 times with 0.155 M NaCl
- Add 3 volume of Depolymerization Solution per volume of packed alginate beads.
- Mix at room temperature for 30 minutes with gentle agitation until the beads have completely dissolved
- Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
- Wash the cells 2 times with 0.155 M NaCl
Media Salts & Chemicals used to Release HC-RD from alginate beads:
- Calcium Chloride Solution: Formulated to induce polymerization of Sodium Alginate.
- Sodium Alginate:Â Encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes
- Depolymerization Solution (DS): Consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Details
| Tissue | Normal healthy human articular cartilage | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Cryovial | N/A | |
| Kit | N/A | |
| Proliferating | Shipped in Differentiation Medium (flasks) | |
| Doublings | N/A | |
| Note | Cells shipped 10 days after encapsulation. Re-Differentiated Chondrocytes expected 3 weeks after encapsulation, retain phenotype at least 1 week after complete re-differentiation. | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.
- Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.
- Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads
- Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.
- Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.
Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads
- Remove Chondrocyte Differentiation Medium
- Wash the beads 2 times with 0.155 M NaCl
- Add 3 volume of Depolymerizatiojn Solution per volume of packed alginate beads
- Mix at room temperature for 30 minutes
- Apply gentle agitation until the beads have completely dissolved
- Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
- Wash the cells 2 times with 0.155 M NaCl
Chondrogenesis Kit (Cat. No. 072K)
| Kit Component | Cat. No. | Storage | ||
|---|---|---|---|---|
| Sodium Alginate Solution | 072-25 | 25C | ||
| Sodium Chloride Solution | 074-100 | 25C | ||
| Calcium Chloride Solution | 071-100 | 25C | ||
| Depolymerization Solution | 073-50 | 25C | ||
| Chondrocyte Differentiation Medium | 411D-250 | 4C |
Antibiotic Antimycotic Solution: AAS
Cat. No. 010-100 / 100 ml
10,000 units/ml Penicillin-G, 10,000 ug/ml Streptomycin Sulfate, and 25 ug/ml Amphotericin in normal saline Sterile filtered solution, 100X, dilute before use
Calcium Chloride Solution: CaCl2
Cat. No. 071-100 / 100 ml
Sterile, ready-to-use
Depolymerization Solution: DS
Cat. No. 073-50 / 50 ml
Sterile-filtered sodium citrate solution, ready-to-use
Hank’s Balanced Salt Solution: HBSS
Cat. No. 062-100 / 100 ml
Sterile 1X solution, ready-to-use
Sodium Alginate Solution
Cat. No. 072-25 / 25 ml
Sterile 1.2% solution, ready-to-use
Sodium Chloride Solution: NaCl
Cat. No. 074-100 / 100 ml
Sterile filtered 0.155 M saline solution, ready-to-use
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