Anti-AKT: Rabbit AKT Antibody

Product Sheet CG1007

Description

BACKGROUND ALK (anaplastic lymphoma kinase) is a tyrosine kinase receptor, expressed as part of the chimeric NPM-ALK protein, in anaplastic large cell lymphomas (ALCLs) exhibiting the t(2;5)(p23;q35) translocation. As a result of this translocation, the NPM (nucleophosmin) gene is fused to the portion of the ALK gene encoding its intracytoplasmic segment. The fusion with NPM results in activation of the ALK kinase domain and its expression in a deregulated and ectopic manner, both in terms of cell type (lymphoid) and cellular compartment (nucleus and cytoplasm).1 In addition, other ALK fusion protein was also discovered in other cancers including non-small cell lung cancer (NSCLC). ALK inhibitor has produced objective response for treatment of such cancer patients in clinical trials. The ALK receptor TK is most closely related to leukocyte tyrosine kinase (LTK), with which it shows 79% amino acid identity in the kinase domain and extensive homology elsewhere, including the ligand-binding domain. Because its ligand has not yet been identified, it remains an orphan receptor, and its normal function is unknown. The normal ALK expression was essentially limited to the central and peripheral nervous system. Other studies suggested that there may be a limited role for ALK outside the nervous system.2 The ALK signaling pathway is gradually being worked out. NPM-ALK has been shown to activate phospholipase C (PLC)-γ, which accounts for much of the mitogenic effect of NPM-ALK, but not its anti-apoptotic effect. By co-immunoprecipitation, NPM-ALK is also has been found to activate the PI3-kinase/AKT pathway. In addition, NPM-ALK interacts directly with Shc and IRS-1, but these interactions were found to be dispensable for transformation. There is also evidence for direct signaling from NPM-ALK to GRB2, but no GRB2 recognition site has been identified in NPM-ALK. Finally, NPM-ALK may also interact with the STAT5 signaling protein and transcription factor.3
 
REFERENCES  
1. Morris, S.W. et al: Sciences 263:1281-4, 1994
2. Lamant, L. et al: Am. J. Pathol. 156:1711-21, 2000

3. Ladanyi, M.: Am. J. Pathol. 157:341-5, 2000

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

(Click to Enlarge) Top: Immunoblotting analysis of extracts from HeLa cells, treated with Insulin 0.01U/ml 15′, using Anti-AKT antibody. The lane on the left was treated with the Anti-AKT antibody. The lane on the right (negative control) was treated with both Anti-AKT antibody and the synthesized immunogen peptide. Middle: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-AKT antibody. Cells on the left were treated with the Anti-AKT antibody. Cells on the right (negative control) were treated with both Anti-AKT antibody and the synthesized immunogen peptide. Bottom: Immunofluorescence of HeLa cells using Anti-AKT antibody. Cells on the left were treated with the Anti-AKT antibody. Cells on the right (negative control) were treated with both Anti-AKT antibody and the synthesized immunogen peptide.

Details

Cat.No.:CG1007
Antigen:Synthesized peptide derived from human AKT
Isotype:Rabbit IgG
Species & predicted
species cross-
reactivity ( ):
Human, Mouse, Rat 
Applications &
Suggested starting
dilutions:*
WB     1:500-1:1000
IP       n/d
IHC     1:50-1:100
ICC     n/d
FACS   n/d
IF        1:100-1:500
Predicted Molecular
Weight of protein:
55 KDa 
Specificity/Sensitivity:Detects endogenous AKT proteins without cross-reactivity with other family members. 
Storage:Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.

Products

Product Size CAT.# Price Quantity
Rabbit AKT Antibody: Rabbit Akt Antibody Size: 100 ul CAT.#: CG1007 Price: $384.00

Resources/Documents

Product Sheet CG1007