Instructions HPAd Normal
Instructions HPAd-T2D
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Adipocytes
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Preadipocytes (HPAd) provide ideal cell models and facilitate life science research and drug discovery for obesity, diabetes and cardiovascular diseases.  We derive HPAd from human subcutaneous and heart adipose tissue and cryopreserve these fibroblast-like precursor cells at the end of primary culture.  They can propagate two passages prior to differentiating into Human Adipocytes (HAd).  Complete differentiation can be easily achieved through the use of Preadipocyte Differentiation Medium.  Mature HAd are expected 10 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation.  Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HPAd are also available prescreened for expression of adipocyte markers.
HPAd from Cell Applications, Inc. have been used in multiple studies investigating the cellular basis of diabetes and obesity, as well as mechanisms of action of various drug candidates.  For example, it was shown that:
  • Interaction between infiltrating monocytes and adipocytes affects production of metalloproteinases and osteopontin, a proinflammatory cytokine, which ultimately leads to development of more adipose tissue and insulin resistance 
  • Adipocyte differentiation requires activation of Akt1 through the mTORC2-BSTA mechanism, leading to downstream suppression of FoxC2
  • Instead of protecting from hyperglycemia-induced ER stress, like it does in other cells, in adipocytes a bioactive, endogenously produced compound taurine was shown to modulate the expression of adipokines under inflammatory conditions by inhibiting the STAT-3 signaling pathway (Kim, 2013a,c), and to inhibit differentiation of preadipocytes into adipocytes 
  • FGF21, which leads to reduction of body weight in animal models of obesity, was shown to act by modulating gene expression, phosphorylating Frs2a, Erk1/2, and Mypt1 and by increasing oxidative capacity of adipocytes via AMPK–SIRT1–PGC1a cascade
  • Atrial natriuretic peptide (ANP) regulates lipid catabolism and reduce insulin resistance in HPAd by activating AMPK
  • Adipocytokines are involved in normal pregnancy and pregnancy-induced hypertension
  • Lotus seed extract exhibits anti-obesity and hypolipidaemic properties
  • HPAd (along with our Human Dermal Fibroblasts) demonstrate the role of epigenetic modifications in increasing efficiency of iPS reprogramming
(Click to Enlarge)  Top: Human Pre-Adipocytes (HPAd).  Bottom: Differentiated Human Adipocytes (HAd) (L)  and Accumulated lipid droplets in HAd, stained by Oil Red O (R).

Details

Tissue
Normal healthy human adipose tissue
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Positive for lipid drops in cytoplasm & Oil Red O Staining, in Differentiation Med
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HPAd (2nd passage) frozen in Basal Medium w/ 20% FBS, 5% DMSO
Kit
Cryovial frozen HPAd (802h/s-05a), Growth Medium (811-500), Subcultr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
Can be cultured 2 passages before differentiation into HAd
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HPAd Normal

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Instructions HPAd-T2D

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HPAd Total Kit: 5x10^5 Cells (from Heart Tissue, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 802hK-05a Price: $629.61
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Cryopreserved HPAd Total Kit: 5x10^5 Cells (from Subcutaneous Tissue, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 802sK-05a Price: $462.76
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Cryopreserved HPAd, adult: Frozen HPAd from Heart Tissue (5x10^5) Size: 1 Cryovial CAT.#: 802h-05a Price: $472.47
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Cryopreserved HPAd, adult: Frozen HPAd from Subcutaneous Tissue (5x10^5) Size: 1 Cryovial CAT.#: 802s-05a Price: $301.35
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Cryopreserved HPAd-h-AS Total Kit: 5x10^5 Cells (from Heart Tissue of donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 802hASK-05a Price: $677.63
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Cryopreserved HPAd-h-AS, adult: Frozen HPAd-h-AS from Heart Tissue of donor with Asthma, Adult (5x10^5) Size: 1 Cryovial CAT.#: 802hAS-05a Price: $521.73
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Cryopreserved HPAd-T2D Total Kit: 5x10^5 Cells (from Heart Tissue of donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 802hT2DK-05a Price: $677.88
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Cryopreserved HPAd-T2D Total Kit: 5x10^5 Cells (from Subcutaneous Tissue of donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 802sT2DK-05a Price: $494.24
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Cryopreserved HPAd-T2D, adult: Frozen HPAd from Heart Tissue of donor with Type 2 Diabetes (5x10^5) Size: 1 Cryovial CAT.#: 802hT2D-05a Price: $521.98
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Cryopreserved HPAd-T2D, adult: Frozen HPAd from Subcutaneous Tissue of donor with Type 2 Diabetes (5x10^5) Size: 1 Cryovial CAT.#: 802sT2D-05a Price: $333.64
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Proliferating HPAd, adult: Actively growing, dividing cells, from subcutaneous tissue, in medium Size: 96 Well CAT.#: 803s-96Wa Price: $634.99
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Proliferating HPAd, adult: Actively growing, dividing cells, from heart tissue, in medium Size: T-25 Flask CAT.#: 803h-25a Price: $472.47
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Proliferating HPAd, adult: Actively growing, dividing cells, from heart tissue, in medium Size: T-75 Flask CAT.#: 803h-75a Price: $676.96
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Proliferating HPAd, adult: Actively growing, dividing cells, from heart tissue, in medium Size: 96 Well CAT.#: 803h-96Wa Price: $806.11
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Proliferating HPAd, adult: Actively growing, dividing cells, from subcutaneous tissue, in medium Size: T-25 Flask CAT.#: 803s-25a Price: $301.35
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Proliferating HPAd, adult: Actively growing, dividing cells, from subcutaneous tissue, in medium Size: T-75 Flask CAT.#: 803s-75a Price: $505.84
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Proliferating HPAd, adult: Actively growing, dividing cells, from subcutaneous tissue, in medium Size: 6 Well CAT.#: 803s-6Wa Price: $505.84
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Proliferating HPAd, adult, Pre-Screened: Actively growing, dividing cells from subcutaneous tissue, in medium, adult Size: 6 Well CAT.#: S803s-6Wa Price: $565.03
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Proliferating HPAd, adult, Pre-Screened: Actively growing, dividing cells from subcutaneous tissue, in medium, adult Size: 96 Well CAT.#: S803s-96Wa Price: $694.18
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Proliferating HPAd, heart: Proliferating HPAd from heart tissue Size: 6 Well CAT.#: 803h-6Wa Price: $676.96
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Related Products

Product Size CAT.# Price Quantity
Human Preadipocyte Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 810-500 Price: $57.04
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Human Preadipocyte Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 811-500 Price: $105.47
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Human Preadipocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 811K-500 Price: $118.39
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Human Preadipocyte Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 811-GS Price: $59.19
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Extended Family Products

Product Size CAT.# Price Quantity
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Preadipocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF802K Price: $349.78
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Cytofect™ Preadipocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF802KS Price: $53.76
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HPAd RNA: Total RNA prepared from Human Preadipocytes, Subcutaneous, adult Size: 10 ug CAT.#: 802s-R10a Price: $349.78
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HPAd RNA: Total RNA prepared from Human Preadipocytes, Subcutaneous, adult Size: 25 ug CAT.#: 802s-R25a Price: $699.56
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Human Adipocyte Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes Size: 250 ml CAT.#: 811D-250 Price: $94.71
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Human Adipocyte Differentiation Medium Kit: Differentiation medium components sold together packaged separately Size: Yields 250 ml CAT.#: 811DS-250 Price: $102.24
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Human Adipocyte Maintenance Medium: Maintenance Medium for adipocytes without insulin Size: 250 ml CAT.#: 811Mi-250 Price: $77.90
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Human Adipocyte Maintenance Medium: Maintenance medium for adipocytes Size: 250 ml CAT.#: 811M-250 Price: $67.80
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Human Adipocyte Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 811S-250 Price: $62.42
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Leptin: Human Leptin Size: 5000 ug CAT.#: RP1037-5000 Price: $538.13
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Leptin: Human Leptin Size: 200 ug CAT.#: RP1037-200 Price: $86.10
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Leptin: Human Leptin Size: 1000 ug CAT.#: RP1037-1000 Price: $193.73
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Leptin ELISA Kit, Human: Human Leptin ELISA Kit Size: 96 wells CAT.#: CL0437 Price: $586.56
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Adipocytes

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

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Publications

2016
Ignacio, R., C. Gibbs, E. Lee and D. Son. 2016. Differential Chemokine Signature between Human Preadipocytes and Adipocytes. Immune Network, 16:189-194.
Ono-Uruga, Y., K. Tozawa, T. Horiuchi, M. Murata, S. Okamoto, Y. Ikeda, T. Suda and Y. Matsubara. 2016. Human adipose tissue-derived stromal cells can differentiate into megakaryocytes and platelets by secreting endogenous thrombopoietin. J Thrombosis & Haemostasis, 6:1285-1297.
Subbaramaiah, K., K. Brown, H. Zahid, K. Balmus, R. Weiss, B. Herbert and A. Dannenberg. 2016. Hsp90 and PKM2 Drive the Expression of Aromatase in Li-Fraumeni Syndrome Breast Adipose Stromal Cells. J Biol Chem, 291:16011-16023.
2015
Fuji, S., K. Fujimoto, N. Goto, M. Kanawa, T. Kawamoto, H. Pan, P. Srivatanakul, W. Rakdang, J. Pornprsitwech, T. Saskianti, K. Suardita, F. Nishimura, and Y. Kato. 2015. Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells. Biomedical Reports, 29 April.
Floyd, E., J. Staszkiewicz, R. Power, G. Kilroy, H. Kirk-Ballard, C. Barnes, K. Strickler, F. Rim, L. Harkins, R. Gao, J. Kim and K. Eilersten. 2015. Prolonged Proteasome Inhibition Cyclically Upregulates Oct3/4 and Nanog Gene Expression, but Reduces Induced Pluripotent Stem Cell Colony Formation. Cellular Reprogramming, 17:95-105.
Guo, F., H. He, Z. Fu, S. Huang, T. Chen, C. Papasian, L. Morse, Y. Xu, R. Battaglino, X. Yang, Z. Jiang, H. Xin, and M. Fu. 2015. Adipocyte-derived PAMM suppresses macrophage inflammation by inhibiting MAPK signaling. Biochem J, DOI: 10.1042/BJ20150019.
Ito, Y., H. Ishiguro, N. Kobayashi, H. Hasumi, M. Watanabe, M. Yao and H. Uemura. 2015. Adipocyte-derived monocyte chemotactic protein-1 (MCP-1) promotes prostate cancer progression through the induction of MMP-2 activity. The Prostate, 75:1009-1019.
Lynch, P. E. Thompson, K. McGinnis, Y. Rovira Gonzalez, J. Surdo, S. Bauer and D. Hursh. 2015. Chromatin Changes at the PPAR-γ2 Promoter During Bone Marrow-Derived Multipotent Stromal Cell Culture Correlate With Loss of Gene Activation Potential. Stem Cells, 33:2169-2181.
You, Y., S. Liu, L. Peng, M. Long, H. Deng and H. Zhao. 2015. Arg972 insulin receptor substrate‑1 enhances tumor necrosis factor‑α‑induced apoptosis in osteoblasts. Molec Med Repts, 12: ISSN:1791-3004. 
2014
Matsubara, Y., T. Zama, Y. Ikeda, Y. Uruga, T. Suda and S. Matsuoka. 2014. Method for producing megakaryocytes, platelets and/or thrombopoietin using mesenchymal cells. Patent Application US 20160177265 A1.
You, J., Y. Lee, K. Kim, S. Kim and K. Chang. 2014. Anti-obesity and hypolipidaemic effects of Nelumbo nucifera seed ethanol extract in human pre-adipocytes and rats fed a high-fat diet. J Science of Food & Ag, 94:568-575.
2013
Kim, K., H.-I. Ji, and H.-I. Yang. 2013a. Taurine May Not Alleviate Hyperglycemia-Mediated Endoplasmic Reticulum Stress in Human Adipocytes. In Taurine 8. Vol. 775. A. El Idrissi and W.J. L'Amoreaux, editors. Springer New York. 395-403.
Kim, K.S., H.M. Choi, H.I. Ji, C. Kim, J.Y. Kim, R. Song, S.M. Kim, Y.A. Lee, S.H. Lee, H.I. Yang, M.C. Yoo, and S.J. Hong. 2013b. Effect of taurine chloramine on differentiation of human preadipocytes into adipocytes. In Advances in experimental medicine and biology. Vol. 775. 247-257.
Kim, K.S., H.I. Ji, H. Chung, C. Kim, S.H. Lee, Y.A. Lee, H.I. Yang, M.C. Yoo, and S.J. Hong. 2013c. Taurine chloramine modulates the expression of adipokines through inhibition of the STAT-3 signaling pathway in differentiated human adipocytes. Amino acids. 45:1415-1422.
Muise, E.S., S. Souza, A. Chi, Y. Tan, X. Zhao, F. Liu, Q. Dallas-yang, M. Wu, T. Sarr, L. Zhu, H. Guo, Z. Li, W. Li, W. Hu, G. Jiang, C.P. Paweletz, R.C. Hendrickson, J.R. Thompson, J. Mu, J.P. Berger, and H. Mehmet. 2013. Downstream Signaling Pathways in Mouse Adipose Tissues Following Acute In Vivo Administration of Fibroblast Growth Factor 21. PloS one. 8:e73011.
Yao, Y., M. Suraokar, B.G. Darnay, B.G. Hollier, T.E. Shaiken, T. Asano, C.-H. Chen, B.H.-J. Chang, Y. Lu, and G.B. Mills. 2013. BSTA Promotes mTORC2-Mediated Phosphorylation of Akt1 to Suppress Expression of FoxC2 and Stimulate Adipocyte Differentiation. Science signaling. 6:ra2.
You, J.S., Y.J. Lee, K.S. Kim, S.H. Kim, and K.J. Chang. 2013. Anti-obesity and hypolipidaemic effects of Nelumbo nucifera seed ethanol extract in human pre-adipocytes and rats fed a high-fat diet. J. Sci. Food & agriculture: doi: 10.1002/jsfa.6297.
2012
Rim, J.S., K.L. Strickler, C.W. Barnes, L.L. Harkins, J. Staszkiewicz, J.M. Gimble, G.H. Leno, and K.J. Eilertsen. 2012. Temporal epigenetic modifications differentially regulate ES cell-like colony formation and maturation. Stem Cell Discovery. 2:45-57.
2011
Naruse, K., Y. Yamasaki, T. Tsunemi, A. Onogi, T. Noguchi, T. Sado, H. Oi, and H. Kobayashi. 2011.  Increase of high molecular weight adiponectin in hypertensive pregnancy was correlated with brain-type natriuretic peptide stimulation on adipocyte. Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health. 1:200-205.
Samuvel, D.J., J. Jin, K.P. Sundararaj, Y. Li, X. Zhang, M.F. Lopes-Virella, and Y. Huang. 2011. TLR4 Activation and IL-6-Mediated Cross Talk between Adipocytes and Mononuclear Cells Synergistically Stimulate MMP-1 Expression. Endocrinology. 152:4662-4671.
2010
Samuvel, D.J., K.P. Sundararaj, Y. Li, M.F. Lopes-Virella, and Y. Huang. 2010. Adipocyte-Mononuclear Cell Interaction, Toll-like Receptor 4 Activation, and High Glucose Synergistically Up-regulate Osteopontin Expression via an Interleukin 6-mediated Mechanism. Journal of Biological Chemistry. 285:3916-3927.
2006
Asai-Sato, M., M. Okamoto, M. Endo, H. Yoshida, M. Murase, M. Ikeda, H. Sakakibara, T. Takahashi, and F. Hirahara. 2006. Hypoadiponectinemia in Lean Lactating Women: Prolactin Inhibits Adiponectin Secretion from Human Adipocytes. Endocrine J, 53:555-562.