Instructions HPBM
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Peripheral Blood Monocytes (HPBM) provide a useful in vitro tool for studying various aspects of pathology and biology.  Our CD14+ Monocytes are isolated and purified from human peripheral blood of healthy donors and cryopreserved immediately after isolation.
HPBM are the precursors of tissue macrophages and dentritic cells and are central to immunological responses, including cell-mediated cytotoxicity and production of inflammatory mediators.  The CD14 monocyte subset expresses a component of the lipopolysaccharide (LPS) receptor complex, are large with diameter of  ~18 μm, represent ~80%–90% of circulating monocytes, and express higher levels of CD62L (L-selectin) and CD11b (also referred to as Mac-1 or CR3) and lower levels of MHC class II than do CD16+ monocytes.
HPBM from Cell Applications, Inc. have been used to
  • Investigate anti-oxidant activity of NF-E2-related transcription factor 2 (NRF2)
  • Identify aldo-keto reductase (AKR) as a tightly regulated human target of NRF2 that can be used as a marker of NRF2 activity

(Click to Enlarge) Human Peripheral Blood CD14+ Monocytes: HPBM

Details

Tissue
Normal healthy human peripheral blood
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
CD14 positive selection
Cryovial
5,000,000 HPBM frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO
Kit
Cryovial Frozen HPBM (6906-50a), Maintenance Medium (615-250)
Cultured
Shipped in Transfer Medium (flasks or plates)
Doublings
N/A - Blood cells don't proliferate in vitro
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HPBM

Format: PDF

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MSDS Cryopreserved Cells

Format: PDF

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Products

Product Size CAT.# Price Quantity
Cryopreserved HPBM Total Kit: 5x10^6 Cells CD14+ Cells (Adult) & Medium (See Details tab for specifics) Size: 1 Kit CAT.#: 6906K-50a Price: $535.17
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Cryopreserved HPBM, adult: Frozen HPBM, CD14+ Size: 1 Cryovial CAT.#: 6906-50a Price: $494.00
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Cultured HPBM, adult: Fresh CD14+ Cells in Transfer Medium Size: T-25 Flask CAT.#: 6907-25a Price: $494.00
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Cultured HPBM, adult: Fresh CD14+ Cells in Transfer Medium Size: T-75 Flask CAT.#: 6907-75a Price: $698.49
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Cultured HPBM, adult: Fresh CD14+ Cells in Transfer Medium Size: 6 Well CAT.#: 6907-6Wa Price: $698.49
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Cultured HPBM, adult: Fresh CD14+ Cells in Transfer Medium Size: 96 Well CAT.#: 6907-96Wa Price: $827.64
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Related Products

Product Size CAT.# Price Quantity
Human Blood Cell Basal Medium: Basal medium (contains no growth supplement).  Add Supplement before use. Size: 250 ml CAT.#: 614-250 Price: $37.67
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Human Blood Cell Culture Medium: Optimized and ready for use. Size: 250 ml CAT.#: 615-250 Price: $65.65
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Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately Size: Yields 250 ml CAT.#: 615K-250 Price: $76.41
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Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium Size: 40 ml CAT.#: 615-CS Price: $39.82
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Human Blood Cell Freezing Medium: Freezing Medium optimized for the unique needs of Blood Cells Size: 50 ml CAT.#: 040-50BC Price: $64.58
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
-+
FLT-3 Ligand: Human FMS-related Tyrosine Kinase 3 Ligand Size: 10 ug CAT.#: RP1021-10 Price: $193.73
-+
FLT-3 Ligand: Human FMS-related Tyrosine Kinase 3 Ligand Size: 100 ug CAT.#: RP1021-100 Price: $484.31
-+
FLT-3 Ligand: Human FMS-related Tyrosine Kinase 3 Ligand Size: 1000 ug CAT.#: RP1021-1000 Price: $4,089.75
-+
FLT-3 Ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 1000 ug CAT.#: RP1021AF-1000 Price: $4,498.73
-+
FLT-3 Ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 10 ug CAT.#: RP1021AF-10 Price: $213.10
-+
FLT-3 Ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free Size: 100 ug CAT.#: RP1021AF-100 Price: $532.74
-+
GM-CSF: Human Granulocyte Macrophage-Colony Stimulating Factor Size: 20 ug CAT.#: RP1008-20 Price: $193.73
-+
GM-CSF: Human Granulocyte Macrophage-Colony Stimulating Factor Size: 100 ug CAT.#: RP1008-100 Price: $484.31
-+
GM-CSF: Human Granulocyte Macrophage-Colony Stimulating Factor Size: 1000 ug CAT.#: RP1008-1000 Price: $3,444.00
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GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 20 ug CAT.#: RP1008AF-20 Price: $213.10
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GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 100 ug CAT.#: RP1008AF-100 Price: $532.74
-+
GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 1000 ug CAT.#: RP1008AF-1000 Price: $3,788.40
-+
Human Blood Cell Freezing Medium: Freezing Medium optimized for the unique needs of Blood Cells Size: 50 ml CAT.#: 040-50BC Price: $64.58
-+
IL-1β: Human Interleukin-1-beta Size: 1000 ug CAT.#: RP1167-1000 Price: $4,089.75
-+
IL-1β: Human Interleukin-1-beta Size: 10 ug CAT.#: RP1167-10 Price: $193.73
-+
IL-1β: Human Interleukin-1-beta Size: 100 ug CAT.#: RP1167-100 Price: $484.31
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IL-1β ELISA Kit, Human: Human Interleukin-1 beta ELISA Kit Size: 96 wells CAT.#: CL0392 Price: $586.56
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IL-1β, Animal-Free: Human Interleukin-1β, Animal-Free Size: 10 ug CAT.#: RP1167AF-10 Price: $213.10
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IL-1β, Animal-Free: Human Interleukin-1β, Animal-Free Size: 100 ug CAT.#: RP1167AF-100 Price: $532.74
-+
IL-1β, Animal-Free: Human Interleukin-1β, Animal-Free Size: 1000 ug CAT.#: RP1167AF-1000 Price: $4,498.73
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $193.73
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.31
-+
IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,174.94
-+
M-CSF: Human Macrophage-Colony Stimulating Factor Size: 10 ug CAT.#: RP1003-10 Price: $193.73
-+
M-CSF: Human Macrophage-Colony Stimulating Factor Size: 100 ug CAT.#: RP1003-100 Price: $624.23
-+
M-CSF: Human Macrophage-Colony Stimulating Factor Size: 1000 ug CAT.#: RP1003-1000 Price: $5,327.44
-+
M-CSF ELISA Kit, Human: Human Macrophage-Colony Stimulating Factor ELISA Kit Size: 96 Wells CAT.#: CL0444 Price: $511.22
-+
M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free Size: 10 ug CAT.#: RP1003AF-10 Price: $213.10
-+
M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free Size: 100 ug CAT.#: RP1003AF-100 Price: $686.65
-+
M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free Size: 1000 ug CAT.#: RP1003AF-1000 Price: $5,860.18
-+
MCP-1 / CCL2: Human Monocyte Chemotactic Protein-1 Size: 20 ug CAT.#: RP1013-20 Price: $193.73
-+
MCP-1 / CCL2: Human Monocyte Chemotactic Protein-1 Size: 100 ug CAT.#: RP1013-100 Price: $484.31
-+
MCP-1 / CCL2: Human Monocyte Chemotactic Protein-1 Size: 1000 ug CAT.#: RP1013-1000 Price: $3,174.94
-+
MCP-1 ELISA Kit, Human: Human Monocyte Chemoattractant Protein-1 ELISA Kit Size: 96 Wells CAT.#: CL0441 Price: $511.22
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MCP-2 / CCL8: Human Monocyte Chemotactic Protein-2 Size: 10 ug CAT.#: RP1130-10 Price: $193.73
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MCP-2 / CCL8: Human Monocyte Chemotactic Protein-2 Size: 100 ug CAT.#: RP1130-100 Price: $484.31
-+
MCP-2 / CCL8: Human Monocyte Chemotactic Protein-2 Size: 1000 ug CAT.#: RP1130-1000 Price: $3,174.94
-+
MCP-3 / CCL7: Human Monocyte Chemotactic Protein-3 Size: 10 ug CAT.#: RP1131-10 Price: $193.73
-+
MCP-3 / CCL7: Human Monocyte Chemotactic Protein-3 Size: 100 ug CAT.#: RP1131-100 Price: $484.31
-+
MCP-3 / CCL7: Human Monocyte Chemotactic Protein-3 Size: 1000 ug CAT.#: RP1131-1000 Price: $3,174.94
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MCP-4 / CCL13: Human Monocyte Chemotactic Protein-4 Size: 20 ug CAT.#: RP1132-20 Price: $193.73
-+
MCP-4 / CCL13: Human Monocyte Chemotactic Protein-4 Size: 100 ug CAT.#: RP1132-100 Price: $484.31
-+
MCP-4 / CCL13: Human Monocyte Chemotactic Protein-4 Size: 1000 ug CAT.#: RP1132-1000 Price: $3,174.94
-+
TNFα: Human Tumor Necrosis Facto-alpha Size: 100 ug CAT.#: RP1111-100 Price: $317.25
-+
TNFα: Human Tumor Necrosis Factor-alpha Size: 10 ug CAT.#: RP1111-10 Price: $78.00
-+
TNFα: Human Tumor Necrosis Factor-alpha Size: 1000 ug CAT.#: RP1111-1000 Price: $1,600.00
-+
TNFα, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free Size: 10 ug CAT.#: RP1111AF-10 Price: $84.50
-+
TNFα, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free Size: 100 ug CAT.#: RP1111AF-100 Price: $346.63
-+
TNFα, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free Size: 1000 ug CAT.#: RP1111AF-1000 Price: $3,492.43
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Cardiovascular Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2011
Chen, D., T. Chen, C. Chien, and P. Li. 2011. Intravenous low redox potential saline attenuates FeCl3-induced vascular dysfunction via downregulation of endothelial H2O2, CX3CL1, intercellular adhesion molecule-1, and p53 expression. J Lab and Clin Med, 157, 306-319.
Tanaka, S., T. Totani, Y. Ishizaki, K. Ota, R. Suenaga, and M. Kuninori. 2011. Multilayer film and cell culture container. Patent Application US 20130164831 A1.