Instructions HAd
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Adipocytes
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Mature Human Adipocytes (HAd) are differentiated from HPAd (Human Preadipocytes) through the use of Adipocyte Differentiation Medium.  Cells are shipped 5 days after initiating differentiation.  Mature HAd are expected 10 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis.  HAd serve as an ideal cellular model for adipogenesis and drug discovery research in the area of obesity and diabetes.
Differentiated HAd from Cell Applications, Inc. have been used to identify genetic elements responsible for aromatase production (and consequently, elevated plasma estrogen) in postmenopausal breast cancer patients.
(Click to Enlarge) Human Adipocytes (HAd) (L). Accumulated lipid droplets in HAd, stained by Oil Red O (R).

Details

Tissue
Normal healthy human adipose tissue
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Positive for lipid drops in cytoplasm
Cryovial
N/A
Kit
N/A
Cultured
Shipped at 1st passage in HAd Diff Med, after 5 days differentiation. Differentiated adipocytes do not divide.
Doublings
N/A
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HAd

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cultured HAd, heart, adult: Differentiated cells in medium Size: T-25 Flask CAT.#: 803hD-25a Price: $306.73
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Cultured HAd, heart, adult: Differentiated cells in medium Size: T-75 Flask CAT.#: 803hD-75a Price: $683.42
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Cultured HAd, heart, adult: Differentiated cells in medium Size: 6 Well CAT.#: 803hD-6Wa Price: $683.42
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Cultured HAd, heart, adult: Differentiated cells in medium Size: 96 Well CAT.#: 803hD-96Wa Price: $769.52
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Cultured HAd, subcutaneous, adult: Differentiated cells in medium Size: T-25 Flask CAT.#: 803sD-25a Price: $188.34
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Cultured HAd, subcutaneous, adult: Differentiated cells in medium Size: T-75 Flask CAT.#: 803sD-75a Price: $532.74
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Cultured HAd, subcutaneous, adult: Differentiated cells in medium Size: 6 Well CAT.#: 803sD-6Wa Price: $532.74
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Cultured HAd, subcutaneous, adult: Differentiated cells in medium Size: 96 Well CAT.#: 803sD-96Wa Price: $618.84
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Related Products

Product Size CAT.# Price Quantity
Human Adipocyte Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes Size: 250 ml CAT.#: 811D-250 Price: $94.71
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Human Adipocyte Differentiation Medium Kit: Differentiation medium components sold together packaged separately Size: Yields 250 ml CAT.#: 811DS-250 Price: $102.24
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Human Adipocyte Maintenance Medium: Maintenance medium for adipocytes Size: 250 ml CAT.#: 811M-250 Price: $67.80
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Human Adipocyte Maintenance Medium: Maintenance Medium for adipocytes without insulin Size: 250 ml CAT.#: 811Mi-250 Price: $77.90
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Extended Family Products

Product Size CAT.# Price Quantity
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Chemerin: Human Chemerin Size: 25 ug CAT.#: RP1036-25 Price: $193.73
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Chemerin: Human Chemerin Size: 100 ug CAT.#: RP1036-100 Price: $484.31
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Chemerin: Human Chemerin Size: 1000 ug CAT.#: RP1036-1000 Price: $3,174.94
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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gACRP30: Human globular ACRP30 / Adipolean Size: 25 ug CAT.#: RP1156-25 Price: $193.73
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gACRP30: Human globular ACRP30 / Adipolean Size: 100 ug CAT.#: RP1156-100 Price: $484.31
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gACRP30: Human globular ACRP30 / Adipolean Size: 1000 ug CAT.#: RP1156-1000 Price: $3,174.94
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HAd RNA: Total RNA prepared from Human Adipocytes, Subcutaneous, adult Size: 10 ug CAT.#: 803sD-R10a Price: $408.98
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HAd RNA: Total RNA prepared from Human Adipocytes, Subcutaneous, adult Size: 25 ug CAT.#: 803sD-R25a Price: $817.95
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HAd RNA: Total RNA prepared from Human Adipocytes, Heart, adult Size: 10 ug CAT.#: 803hD-R10a Price: $408.98
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HAd RNA: Total RNA prepared from Human Adipocytes, Heart, adult Size: 25 ug CAT.#: 803hD-R25a Price: $817.95
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Human Adipocyte Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 811S-250 Price: $62.42
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Leptin: Human Leptin Size: 200 ug CAT.#: RP1037-200 Price: $86.10
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Leptin: Human Leptin Size: 1000 ug CAT.#: RP1037-1000 Price: $193.73
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Leptin: Human Leptin Size: 5000 ug CAT.#: RP1037-5000 Price: $538.13
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Leptin ELISA Kit, Human: Human Leptin ELISA Kit Size: 96 wells CAT.#: CL0437 Price: $586.56
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Resistin: Human Resistin Dimer Size: 25 ug CAT.#: RP1107-25 Price: $193.73
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Resistin: Human Resistin Dimer Size: 100 ug CAT.#: RP1107-100 Price: $624.23
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Resistin: Human Resistin Dimer Size: 1000 ug CAT.#: RP1107-1000 Price: $5,327.44
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Resistin ELISA Kit, Human: Human Resistin ELISA Kit Size: 96 wells CAT.#: CL0581 Price: $608.08
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Adipocytes

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2016
Duncan, D., T. Kamerzell and M. Palmer. 2016. Use of Indole Compounds for Fat Reduction and Skin and Soft Tissue Tightening.  Patent Applications US 20160030389 A1.
Ignacio, R., C. Gibbs, E. Lee and D. Son. 2016. Differential Chemokine Signature between Human Preadipocytes and Adipocytes. Immune Network, 16:189-194.
2013
Liu, M., J.N. Ingle, B.L. Fridley, A.U. Buzdar, M.E. Robson, M. Kubo, L. Wang, A. Batzler, G.D. Jenkins, T.L. Pietrzak, E.E. Carlson, M.P. Goetz, D.W. Northfelt, E.A. Perez, C.V. Williard, D.J. Schaid,
Y. Nakamura, and R.M. Weinshilboum. 2013. TSPYL5 SNPs: Association with Plasma Estradiol Concentrations and Aromatase Expression. Mol. endocrinology. 27:657-670.
2011
Medici, D., and B. Olsen. 2011. Conversion of vascular endothelial cells into multipotent stem-like cells. Patent Application US 20130078718 A1.
Souza, S.C., M.D.L. Chau, Q. Yang, M.-S. Gauthier, K.B. Clairmont, Z. Wu, J. Gromada, and W.P. Dole. 2011. Atrial natriuretic peptide regulates lipid mobilization and oxygen consumption in human adipocytes by activating AMPK. Biochemical and biophysical research communications. 410:398-403.
2010
Chau, D., J. Gromada, and Z Wu. Methods of treating cancers. Patent Application US 20120076729 A1.
Chau, M.D.L., J. Gao, Q. Yang, Z. Wu, and J. Gromada. 2010. Fibroblast growth factor 21 regulates energy metabolism by activating the AMPK–SIRT1–PGC-1α pathway. Proceedings of the National Academy of Sciences. 107:12553-12558.
2006
Baron, U., I. Turbachova, A. Hellwag, F. Eckardt, K. Berlin, U. Hoffmüller, P. Gardina, and S. Olek. 2006.  DNA Methylation Analysis as a Tool for Cell Typing. Epigenetics 1:55-60.