Instructions HAd
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Adipocytes
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Mature Human Adipocytes (HAd) are differentiated from HPAd (Human Preadipocytes) through the use of Adipocyte Differentiation Medium. Cells are shipped 5 days after initiating differentiation. Mature HAd are expected 10 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HAd serve as an ideal cellular model for adipogenesis and drug discovery research in the area of obesity and diabetes.
Differentiated HAd from Cell Applications, Inc. have been used to identify genetic elements responsible for aromatase production (and consequently, elevated plasma estrogen) in postmenopausal breast cancer patients.
(Click to Enlarge) Human Adipocytes (HAd) (L). Accumulated lipid droplets in HAd, stained by Oil Red O (R).
Details
Tissue | Normal healthy human adipose tissue |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Positive for lipid drops in cytoplasm |
Cryovial | N/A |
Kit | N/A |
Cultured | Shipped at 1st passage in HAd Diff Med, after 5 days differentiation. Differentiated adipocytes do not divide. |
Doublings | N/A |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cultured Adipocytes, Subcutaneous (HAd), adult: Differentiated cells in medium | Size: T-25 Flask | CAT.#: 803sD-25a | Price: $343.00 | |
| Cultured Adipocytes, Subcutaneous (HAd), adult: Differentiated cells in medium | Size: T-75 Flask | CAT.#: 803sD-75a | Price: $533.00 | |
| Cultured Adipocytes, Subcutaneous (HAd), adult: Differentiated cells in medium | Size: 24 Well | CAT.#: 803sD-24Wa | Price: $533.00 | |
| Cultured Adipocytes, Subcutaneous (HAd), adult: Differentiated cells in medium | Size: 96 Well | CAT.#: 803sD-96Wa | Price: $619.00 | |
| Cultured Adipocytes, Heart (HAd), adult: Differentiated cells in medium | Size: T-25 Flask | CAT.#: 803hD-25a | Price: $307.00 | |
| Cultured Adipocytes, Heart (HAd), adult: Differentiated cells in medium | Size: T-75 Flask | CAT.#: 803hD-75a | Price: $683.00 | |
| Cultured Adipocytes, Heart (HAd), adult: Differentiated cells in medium | Size: 24 Well | CAT.#: 803hD-24Wa | Price: $683.00 | |
| Cultured Adipocytes, Heart (HAd), adult: Differentiated cells in medium | Size: 96 Well | CAT.#: 803hD-96Wa | Price: $770.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HAd Maintenance Medium: Maintenance medium for adipocytes | Size: 250 ml | CAT.#: 811M-250 | Price: $78.00 | |
| HAd Maintenance Medium wo Insulin: Maintenance Medium for adipocytes without insulin | Size: 250 ml | CAT.#: 811Mi-250 | Price: $90.00 | |
| HAd Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment | Size: 250 ml | CAT.#: 811S-250 | Price: $71.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Adipocyte RNA, Subcutaneous (HAd-s RNA), Adult: Total RNA prepared from Human Adipocytes, Subcutaneous, adult | Size: 10 ug | CAT.#: 803sD-R10a | Price: $465.00 | |
| Adipocyte RNA, Subcutaneous (HAd-s RNA), Adult: Total RNA prepared from Human Adipocytes, Subcutaneous, adult | Size: 25 ug | CAT.#: 803sD-R25a | Price: $930.00 | |
| Adipocyte RNA, Heart (HAd-h RNA), Adult: Total RNA prepared from Human Adipocytes, Heart, adult | Size: 10 ug | CAT.#: 803hD-R10a | Price: $465.00 | |
| Adipocyte RNA, Heart (HAd-h RNA), Adult: Total RNA prepared from Human Adipocytes, Heart, adult | Size: 25 ug | CAT.#: 803hD-R25a | Price: $930.00 | |
| HPAd Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes | Size: 250 ml | CAT.#: 811D-250 | Price: $109.00 | |
| HPAd Differentiation Medium Component Kit: Differentiation medium components sold together packaged separately | Size: Yields 250 ml | CAT.#: 811DS-250 | Price: $152.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Human Chemerin: Human Chemerin | Size: 25 ug | CAT.#: RP1036-25 | Price: $194.00 | |
| Human Chemerin: Human Chemerin | Size: 100 ug | CAT.#: RP1036-100 | Price: $484.00 | |
| Human Chemerin: Human Chemerin | Size: 1000 ug | CAT.#: RP1036-1000 | Price: $3,175.00 | |
| Human globular subunit of adipocyte complement-related protein (ACRP30 / Adipolean): Human globular ACRP30 / Adipolean | Size: 25 ug | CAT.#: RP1156-25 | Price: $194.00 | |
| Human globular subunit of adipocyte complement-related protein (ACRP30 / Adipolean): Human globular ACRP30 / Adipolean | Size: 100 ug | CAT.#: RP1156-100 | Price: $484.00 | |
| Human globular subunit of adipocyte complement-related protein (ACRP30 / Adipolean): Human globular ACRP30 / Adipolean | Size: 1000 ug | CAT.#: RP1156-1000 | Price: $3,175.00 | |
| Human Leptin: Human Leptin | Size: 200 ug | CAT.#: RP1037-200 | Price: $86.00 | |
| Human Leptin: Human Leptin | Size: 1000 ug | CAT.#: RP1037-1000 | Price: $194.00 | |
| Human Leptin: Human Leptin | Size: 5000 ug | CAT.#: RP1037-5000 | Price: $538.00 | |
| Human Leptin ELISA Kit: Human Leptin ELISA Kit | Size: 96 wells | CAT.#: CL0437 | Price: $587.00 | |
| Human Resistin Dimer: Human Resistin Dimer | Size: 25 ug | CAT.#: RP1107-25 | Price: $194.00 | |
| Human Resistin Dimer : Human Resistin Dimer | Size: 100 ug | CAT.#: RP1107-100 | Price: $624.00 | |
| Human Resistin Dimer: Human Resistin Dimer | Size: 1000 ug | CAT.#: RP1107-1000 | Price: $5,327.00 | |
| Human Resistin ELISA Kit: Human Resistin ELISA Kit | Size: 96 wells | CAT.#: CL0581 | Price: $608.00 |
Resources/Documents
Publications
2016
Duncan, D., T. Kamerzell and M. Palmer. 2016. Use of Indole Compounds for Fat Reduction and Skin and Soft Tissue Tightening. Patent Applications US 20160030389 A1.
Ignacio, R., C. Gibbs, E. Lee and D. Son. 2016. Differential Chemokine Signature between Human Preadipocytes and Adipocytes. Immune Network, 16:189-194.
2013
Liu, M., J.N. Ingle, B.L. Fridley, A.U. Buzdar, M.E. Robson, M. Kubo, L. Wang, A. Batzler, G.D. Jenkins, T.L. Pietrzak, E.E. Carlson, M.P. Goetz, D.W. Northfelt, E.A. Perez, C.V. Williard, D.J. Schaid,
Y. Nakamura, and R.M. Weinshilboum. 2013. TSPYL5 SNPs: Association with Plasma Estradiol Concentrations and Aromatase Expression. Mol. endocrinology. 27:657-670.
2011
Medici, D., and B. Olsen. 2011. Conversion of vascular endothelial cells into multipotent stem-like cells. Patent Application US 20130078718 A1.
Souza, S.C., M.D.L. Chau, Q. Yang, M.-S. Gauthier, K.B. Clairmont, Z. Wu, J. Gromada, and W.P. Dole. 2011. Atrial natriuretic peptide regulates lipid mobilization and oxygen consumption in human adipocytes by activating AMPK. Biochemical and biophysical research communications. 410:398-403.
2010
Chau, D., J. Gromada, and Z Wu. Methods of treating cancers. Patent Application US 20120076729 A1.
Chau, M.D.L., J. Gao, Q. Yang, Z. Wu, and J. Gromada. 2010. Fibroblast growth factor 21 regulates energy metabolism by activating the AMPK–SIRT1–PGC-1α pathway. Proceedings of the National Academy of Sciences. 107:12553-12558.
2006
Baron, U., I. Turbachova, A. Hellwag, F. Eckardt, K. Berlin, U. Hoffmüller, P. Gardina, and S. Olek. 2006. DNA Methylation Analysis as a Tool for Cell Typing. Epigenetics 1:55-60.