Instructions RSC
5 Important Cell Culture Rules
Cell Apps Flyer Nervous System
MSDS Cryopreserved Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Schwann Cells (RSC) are the principle glial cells in the peripheral nervous system, providing physical and chemical supports for neurons. Myelinating Schwann cells surround neuronal cell bodies (sensory ganglia) and fibers (sensory and motor neurons) to form an insulating sheath. Periodic exposure of the axonal membrane to the extracellular space at the nodes of Raniver (myelin sheath gaps) allows rapid propagation of action potentials along the axon. RSC are important for morphology, survival and growth of axons & neurons. Studies also indicate Schwann cells promote neuronal restoration and experimental spinal cord regeneration.
The calcium binding protein S100β, a marker present in cultured Schwann cells, participates in paracrine mechanisms and neuronal maintenance. S100β exerts neurotrophic effects to both peripheral sensory and motor neurons, and demonstrates experimental promise to repair, restore or treat nerve injuries and CNS lesions.
Characterization: Rat Schwann Cells from every lot stain Positive for S100β, attach & spread on tissue culture ware and proliferate in Rat Schwann Cell Growth Medium.
(Click to Enlarge) Rat Schwann Cells: RSC phase contrast image (Left) and RSC stained red for schwann cell marker S100β and nuclei counter-stained blue with DAPI (Right)
Details
| Tissue | Normal healthy adult rat peripheral nerves |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for S100β |
| Bioassay | Attach, spread, proliferate in Gr Med |
| Cryovial | 500,000 RSC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | R842K-05a: Cryovial frozen RSC (R842-05a), Growth Medium (R825-500), Coating Solution (036-20)* Subcltr Rgnt Kit (090K). *036-20-J in R842K-05a-J |
| Proliferating | Shipped in Gr Med, 1st psg (flasks or plates) |
| Doublings | At least 3 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Schwann Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R842K-05a | Price: $644.00 | |
| Cryopreserved Rat Schwann Cells (RSC), adult: Frozen RSC (5x10^5) | Size: 1 Cryovial | CAT.#: R842-05a | Price: $420.00 | |
| Proliferating Rat Schwann Cells (RSC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R843-25a | Price: $420.00 | |
| Proliferating Rat Schwann Cells (RSC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R843-96Wa | Price: $730.00 | |
| Proliferating Rat Schwann Cells (RSC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R843-75a | Price: $610.00 | |
| Proliferating Rat Schwann Cells (RSC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R843-24Wa | Price: $610.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Schwann Growth Medium: All-in-one ready-to-use media for Rat Schwann Cells | Size: 500 ml | CAT.#: R825-500 | Price: $171.00 | |
| Rat Schwann Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: R825K-500 | Price: $183.00 | |
| Rat Schwann Cell Basal Medium, 500ml: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R824-500 | Price: $60.00 | |
| Rat Schwann Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: R825-GS | Price: $166.00 | |
| Schwann Cell Coating Solution: Coating solution | Size: 20 ml | CAT.#: 036-20 | Price: $59.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse S100B Antibody: Mouse S100B Antibody | Size: 100 ul | CAT.#: CP10223 | Price: $333.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Cryopreserved RSC, Adult: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R842K-05a-J | Price: $644.00 | |
| Schwann Cell Coating Solution: Coating solution | Size: 20 ml | CAT.#: 036-20-J | Price: $68.00 |
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