Instructions RSC
5 Important Cell Culture Rules
Cell Apps Flyer Nervous System
MSDS Cryopreserved Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Schwann Cells (RSC) are the principle glial cells in the peripheral nervous system, providing physical and chemical supports for neurons.  Myelinating Schwann cells surround neuronal cell bodies (sensory ganglia) and fibers (sensory and motor neurons) to form an insulating sheath. Periodic exposure of the axonal membrane to the extracellular space at the nodes of Raniver (myelin sheath gaps) allows rapid propagation of action potentials along the axon.  RSC are important for morphology, survival and growth of axons & neurons.  Studies also indicate Schwann cells promote neuronal restoration and experimental spinal cord regeneration.
The calcium binding protein S100β, a marker present in cultured Schwann cells, participates in paracrine mechanisms and neuronal maintenance.  S100β exerts neurotrophic effects to both peripheral sensory and motor neurons, and demonstrates experimental promise to repair, restore or treat nerve injuries and CNS lesions.
Characterization: Rat Schwann Cells from every lot stain Positive for S100β, attach & spread on tissue culture ware and proliferate in Rat Schwann Cell Growth Medium.
(Click to Enlarge) Rat Schwann Cells: RSC phase contrast image (Left) and RSC stained red for schwann cell marker S100β and nuclei counter-stained blue with DAPI (Right)

Details

TissueNormal healthy adult rat peripheral nerves
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for S100β
BioassayAttach, spread, proliferate in Gr Med
Cryovial500,000 RSC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
KitCryovial frozen RSC (R842-05a), Growth Medium (R825-500), Subcltr Rgnt Kit (090K)
ProliferatingShipped in Gr Med, 1st psg (flasks or plates)
DoublingsAt least 3
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RSC

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Products

Product Size CAT.# Price Quantity
Cryopreserved RSC: Frozen RSC (5x10^5) Size: 1 Cryovial CAT.#: R842-05a Price: $355.68
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Cryopreserved RSC Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R842K-05a Price: $426.81
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Proliferating RSC: Actively growing, dividing cells in medium Size: 6 Well CAT.#: R843-6Wa Price: $462.38
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Proliferating RSC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: R843-75a Price: $462.38
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Proliferating RSC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: R843-96Wa Price: $533.51
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Proliferating RSC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: R843-25a Price: $355.68
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Related Products

Product Size CAT.# Price Quantity
Rat Schwann Cell Basal Medium: Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: R824-500 Price: $46.13
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Rat Schwann Cell Growth Medium: All-in-one ready-to-use media Size: 500 ml CAT.#: R825-500 Price: $148.63
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Rat Schwann Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: R825K-500 Price: $158.88
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Rat Schwann Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: R825-GS Price: $128.13
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-S100B: Mouse S100B Antibody Size: 100 ul CAT.#: CP10223 Price: $333.13
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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