Instructions HDC-PB
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description

Human Dendritic Cells from Peripheral Blood (HDC-PB) arise from a distinct hematopoietic lineage and help regulate the immune system.  They mount adaptive immune responses to foreign antigens, “prime” T cells through antigen presentation and can induce tolerance to self-antigens.  Academic & industrial research is driving toward potential therapeutic benefits of modulating DCs for vaccines and suppressive therapies against pathogens, tumors and autoimmune diseases.  HDC constitutively express CD11c, a type I transmembrane protein also known as Integrin Alpha X (ITGAX).
(Click to Enlarge) Human Dendritic Cells from Peripheral Blood (HDC-PB) phase contrast (L) and flourescent imaging (R) of cells stained positive for the type I transmembrane protein CD11c (Integrin Alpha X), a hematopoietic marker constitutively expressed on the HDC surface.

Details

TissueNormal healthy human peripheral blood
QCNo bacteria, yeast, fungi, mycoplasma or virus
CharacterPositive for CD11c. Negative for F4/80.
BioassayMaintains in TC ware 7 days
Cryovial500,000 HDC-PB frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO
DoublingsN/A - Blood cells don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HDC-PB

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Products

Product Size CAT.# Price Quantity
Cryopreserved HDC-PB, Adult: Frozen HDC-PB (5x10^5) Size: 1 Cryovial CAT.#: 6908-05a Price: $562.73
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Related Products

Product Size CAT.# Price Quantity
Human Dendritic Cell Basal Medium: Basal medium (contains no growth supplement). Add Supplement before use. Size: 50 ml CAT.#: 622-50 Price: $15.38
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Human Dendritic Cell Culture Medium: Optimized and ready for use Size: 50 ml CAT.#: 623-50 Price: $153.75
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Human Dendritic Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately Size: Yields 50 ml CAT.#: 623K-50 Price: $158.88
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Human Dendritic Cell Culture Supplement: Add to Basal Medium to create Culture Medium Size: 6 ml CAT.#: 623-CS Price: $143.50
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Human Hematopoietic Cell Freezing Medium: Serum-free freezing medium optimized for CD34+ Hematopoietic Stem Cells (HHSC) Size: 50 ml CAT.#: 044-50 Price: $74.26
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now