Instructions HDC-PB
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description
Human Dendritic Cells from Peripheral Blood (HDC-PB) arise from a distinct hematopoietic lineage and help regulate the immune system. They mount adaptive immune responses to foreign antigens, “prime” T cells through antigen presentation and can induce tolerance to self-antigens. Academic & industrial research is driving toward potential therapeutic benefits of modulating DCs for vaccines and suppressive therapies against pathogens, tumors and autoimmune diseases. HDC constitutively express CD11c, a type I transmembrane protein also known as Integrin Alpha X (ITGAX).
(Click to Enlarge) Human Dendritic Cells from Peripheral Blood (HDC-PB) phase contrast (L) and flourescent imaging (R) of cells stained positive for the type I transmembrane protein CD11c (Integrin Alpha X), a hematopoietic marker constitutively expressed on the HDC surface.
Details
| Tissue | Normal healthy human peripheral blood |
| QC | No bacteria, yeast, fungi, mycoplasma or virus |
| Character | Positive for CD11c. Negative for F4/80. |
| Bioassay | Maintains in TC ware 7 days |
| Cryovial | 500,000 HDC-PB frozen right after isolation in Freezing Medium w/ 10% FBS, 10% DMSO |
| Doublings | N/A - Blood cells don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Dendritic Cells-Peripheral Blood (HDC-PB), adult: Frozen HDC-PB (5x10^5) | Size: 1 Cryovial | CAT.#: 6908PB-05a | Price: $925.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HDC Basal Medium: Basal medium (contains no growth supplement). Add Supplement before use. | Size: 50 ml | CAT.#: 622-50 | Price: $20.00 | |
| HDC Culture Medium: Optimized and ready for use | Size: 50 ml | CAT.#: 623-50 | Price: $177.00 | |
| HDC Culture Medium Kit: Basal medium & supplement sold together packaged separately | Size: Yields 50 ml | CAT.#: 623K-50 | Price: $183.00 | |
| HDC Culture Supplement: Add to Basal Medium to create Culture Medium | Size: 6 ml | CAT.#: 623-CS | Price: $187.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Freezing Medium for CD34+ Hematopoietic Stem Cells: Serum-free freezing medium optimized for CD34+ Hematopoietic Stem Cells (HHSC), Dendritic Cells-Peripheral Blood (HDC-PB) | Size: 50 ml | CAT.#: 044-50 | Price: $85.00 |